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Conference Paper: Peptide Hydrogel Scaffold Promotes Angiogenesis And Osteo/Odontogenic Differentiation In-Vitro

TitlePeptide Hydrogel Scaffold Promotes Angiogenesis And Osteo/Odontogenic Differentiation In-Vitro
Authors
KeywordsBiomaterials
Pulp
Regeneration
Stem Cells and Tissue engineering
Issue Date2013
PublisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The 91st General Session & Exhibition of the International Association for Dental Research (IADR), Seattle, Washington, USA, 20-23 March 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue A: abstract no. 3605 How to Cite?
AbstractObjectives: To investigate cell viability, vascular network formation and differentiation of mono- or co-cultured human dental pulp stem cells (DPSCs) and human umbilical vein endothelial cells (HUVECs) within a three-dimensional peptide hydrogel scaffold (PHS). Methods: Monocultures and combinations of co-cultures (3:1, 1:1, 1:3) of DPSCs and HUVECs were encapsulated in different concentrations (0.5%, 0.25%, 0.15%) of PHS (BD-Biosciences, Bedford, MA). DPSCs and HUVECs were transfected with green fluorescent protein and red fluorescent protein respectively using pre-made lentiviral particles (GenTarget Inc, San Diego, CA) before encapsulation. Cellular morphologies and 3-dimensional organization of cultures within PHS were monitored using confocal microscopy. Cell viability was assessed using a live/dead viability assay kit at day 7 and 14. Cell cultures within PHS were induced for odonto/osteogenic differentiation (up to 21-days), examined for alkaline phosphatase (ALP) activity (ALP quantification assay) and mineralization (Von-Kossa staining). Experiments were conducted in triplicate using DPSCs from three different donors and statistically analysed (ANOVA). Results: Live/dead assay revealed 0.15% as the optimum PHS concentration with a significantly high survival of HUVECs compared to 0.5% and 0.25% PHS. In monocultures, DPSCs survived and grew faster than HUVECs. In co-cultures both cells survived well and HUVECs formed an extensive vessel-like network throughout the PHS compared to HUVEC monocultures where it failed to form any vessel-like structures. This finding suggested that co-culture inhibits HUVECs apoptosis and secretes angiogenic factors to promote vessel-like network formation. At higher endothelial cell counts, the density of vessel-like structures was low and DPSC:HUVEC 3:1 was found as the optimum ratio. ALP activity of cells in co-culture was higher than that of DPSCs in monocultures (p<0.05). Despite the ratio, all the co-cultures showed higher amount of mineralization compared to monocultures. Conclusion: These findings indicate that PHS promotes angiogenesis and osteo/odontogenic differentiation and has potential for engineering vascularised-pulp tissues.
DescriptionPoster Presentation
Session 406: Oral Tissues
Persistent Identifierhttp://hdl.handle.net/10722/183221
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorDissanayaka, WLen_US
dc.contributor.authorZhang, Cen_US
dc.contributor.authorHargreaves, Ken_US
dc.contributor.authorJin, Len_US
dc.date.accessioned2013-05-15T01:48:19Z-
dc.date.available2013-05-15T01:48:19Z-
dc.date.issued2013en_US
dc.identifier.citationThe 91st General Session & Exhibition of the International Association for Dental Research (IADR), Seattle, Washington, USA, 20-23 March 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue A: abstract no. 3605en_US
dc.identifier.issn0022-0345en_US
dc.identifier.urihttp://hdl.handle.net/10722/183221-
dc.descriptionPoster Presentation-
dc.descriptionSession 406: Oral Tissues-
dc.description.abstractObjectives: To investigate cell viability, vascular network formation and differentiation of mono- or co-cultured human dental pulp stem cells (DPSCs) and human umbilical vein endothelial cells (HUVECs) within a three-dimensional peptide hydrogel scaffold (PHS). Methods: Monocultures and combinations of co-cultures (3:1, 1:1, 1:3) of DPSCs and HUVECs were encapsulated in different concentrations (0.5%, 0.25%, 0.15%) of PHS (BD-Biosciences, Bedford, MA). DPSCs and HUVECs were transfected with green fluorescent protein and red fluorescent protein respectively using pre-made lentiviral particles (GenTarget Inc, San Diego, CA) before encapsulation. Cellular morphologies and 3-dimensional organization of cultures within PHS were monitored using confocal microscopy. Cell viability was assessed using a live/dead viability assay kit at day 7 and 14. Cell cultures within PHS were induced for odonto/osteogenic differentiation (up to 21-days), examined for alkaline phosphatase (ALP) activity (ALP quantification assay) and mineralization (Von-Kossa staining). Experiments were conducted in triplicate using DPSCs from three different donors and statistically analysed (ANOVA). Results: Live/dead assay revealed 0.15% as the optimum PHS concentration with a significantly high survival of HUVECs compared to 0.5% and 0.25% PHS. In monocultures, DPSCs survived and grew faster than HUVECs. In co-cultures both cells survived well and HUVECs formed an extensive vessel-like network throughout the PHS compared to HUVEC monocultures where it failed to form any vessel-like structures. This finding suggested that co-culture inhibits HUVECs apoptosis and secretes angiogenic factors to promote vessel-like network formation. At higher endothelial cell counts, the density of vessel-like structures was low and DPSC:HUVEC 3:1 was found as the optimum ratio. ALP activity of cells in co-culture was higher than that of DPSCs in monocultures (p<0.05). Despite the ratio, all the co-cultures showed higher amount of mineralization compared to monocultures. Conclusion: These findings indicate that PHS promotes angiogenesis and osteo/odontogenic differentiation and has potential for engineering vascularised-pulp tissues.-
dc.languageengen_US
dc.publisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925en_US
dc.relation.ispartofJournal of Dental Researchen_US
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.en_US
dc.subjectBiomaterials-
dc.subjectPulp-
dc.subjectRegeneration-
dc.subjectStem Cells and Tissue engineering-
dc.titlePeptide Hydrogel Scaffold Promotes Angiogenesis And Osteo/Odontogenic Differentiation In-Vitroen_US
dc.typeConference_Paperen_US
dc.identifier.emailZhang, C: zhangcf@hku.hken_US
dc.identifier.emailJin, L: ljjin@hkucc.hku.hken_US
dc.identifier.authorityZhang, C=rp01408en_US
dc.identifier.authorityJin, L=rp00028en_US
dc.identifier.hkuros214400en_US
dc.identifier.volume92en_US
dc.identifier.issueSpecial Issue A: abstract no. 3605en_US
dc.publisher.placeUnited States-

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