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Conference Paper: Pitx2 Overexpression: Effects on the Early Development of Zebrefish Teeth

TitlePitx2 Overexpression: Effects on the Early Development of Zebrefish Teeth
Authors
KeywordsGene expression and Regeneration
Issue Date2013
PublisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The 91st General Session & Exhibition of the International Association for Dental Research (IADR), Seattle, Washington, USA, 20-23 March 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue A: abstract no. 3495 How to Cite?
AbstractObjectives: To address the effects of pitx2 overexpression on the early development of teeth in zebrafish. Methods: The mRNA of pitx2 was synthesized in vitro via cloning the CDS of pitx2 by RT-PCR. The DNA fragment was subjected to adding in restriction enzyme sites to become a template. The zebrafish embryos were divided into four experimental groups, with each embryo accepting a microinjection of pitx2 mRNA to establish the overexpression model. Development of embryos within 72hr was observed under microscope. Semi-quantitative RT-PCR and grayscale value analysis were adapted to detect possible changes in the gene expression related to the tooth development. Results: Morphological observation of 72-hour embryos of zebrafish revealed arrest developments, pericardium edema, notochord deformation, and eye malformation. Semi-quantitative RT-PCR and grayscale value analysis indicated that over-expression of pitx2 down-regulated bmp4, while up-regulated fgf8, dlx2b, and lef1. No significant effect was detected in the expression levels of runx2a. Conclusions: Over-expression of pitx2 could result in morphological changes in the embryos of zebrafish. Genes related to early development of teeth of zebrafish were up- or down-regulated, or not effected by pitx2 overexpression. The changes were similar as what we observed in mice.
DescriptionPoster Presentation
Session 399: Pulp Biology II
Persistent Identifierhttp://hdl.handle.net/10722/183220
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorLu, Qen_US
dc.contributor.authorJin, Len_US
dc.date.accessioned2013-05-15T01:48:19Z-
dc.date.available2013-05-15T01:48:19Z-
dc.date.issued2013en_US
dc.identifier.citationThe 91st General Session & Exhibition of the International Association for Dental Research (IADR), Seattle, Washington, USA, 20-23 March 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue A: abstract no. 3495en_US
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/183220-
dc.descriptionPoster Presentation-
dc.descriptionSession 399: Pulp Biology II-
dc.description.abstractObjectives: To address the effects of pitx2 overexpression on the early development of teeth in zebrafish. Methods: The mRNA of pitx2 was synthesized in vitro via cloning the CDS of pitx2 by RT-PCR. The DNA fragment was subjected to adding in restriction enzyme sites to become a template. The zebrafish embryos were divided into four experimental groups, with each embryo accepting a microinjection of pitx2 mRNA to establish the overexpression model. Development of embryos within 72hr was observed under microscope. Semi-quantitative RT-PCR and grayscale value analysis were adapted to detect possible changes in the gene expression related to the tooth development. Results: Morphological observation of 72-hour embryos of zebrafish revealed arrest developments, pericardium edema, notochord deformation, and eye malformation. Semi-quantitative RT-PCR and grayscale value analysis indicated that over-expression of pitx2 down-regulated bmp4, while up-regulated fgf8, dlx2b, and lef1. No significant effect was detected in the expression levels of runx2a. Conclusions: Over-expression of pitx2 could result in morphological changes in the embryos of zebrafish. Genes related to early development of teeth of zebrafish were up- or down-regulated, or not effected by pitx2 overexpression. The changes were similar as what we observed in mice.-
dc.languageengen_US
dc.publisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925-
dc.relation.ispartofJournal of Dental Researchen_US
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.-
dc.subjectGene expression and Regeneration-
dc.titlePitx2 Overexpression: Effects on the Early Development of Zebrefish Teethen_US
dc.typeConference_Paperen_US
dc.identifier.emailJin, L: ljjin@hkucc.hku.hken_US
dc.identifier.authorityJin, L=rp00028en_US
dc.identifier.hkuros214399en_US
dc.identifier.volume92en_US
dc.identifier.issueSpecial Issue A: abstract no. 3495en_US
dc.publisher.placeUnited States-

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