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Conference Paper: Novel S. Mutans Detection System in Predicting Early Childhood Caries
Title | Novel S. Mutans Detection System in Predicting Early Childhood Caries |
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Authors | |
Keywords | Caries Children Immunology S. mutans and Saliva |
Issue Date | 2013 |
Publisher | Sage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925 |
Citation | The 91st General Session & Exhibition of the International Association for Dental Research (IADR), Seattle, Washington, USA, 20-23 March 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue A: abstract no. 2604 How to Cite? |
Abstract | Objectives: Abundance of S. mutans in saliva is an important microbiological parameter for assessing caries risk. A novel monoclonal antibody-based immunoassay (Saliva-Check mutans) was developed and outperformed the conventional culture-based assay (Dentocult SM) in enumerating S. mutans in saliva. This study aimed to evaluate and compare their validity in predicting early childhood caries (ECC). Methods: With ethical approval and parental written consent, 190 preschool children aged 3-4 years were recruited. The abundance of S. mutans in their stimulated saliva samples was classified by the culture-based assay into four levels (0-3), while the immunoassay categorized the samples into two groups with high and low S. mutans levels. Children's tooth status was assessed at baseline and after 12 months. Caries increment (△dmft) was recorded. The validity of both assays in predicting new caries (△dmft>0) was evaluated and benchmarked against that of 'past caries', which is regarded as the strongest indicator for future caries. Results: The mean caries increment was higher in children with 'past caries' than in those caries-free at baseline (1.46 vs. 0.25; p<0.001). Compared with children classified by the immunoassay as harboring low level of S. mutans, children with high level of S. mutans developed more new caries (1.66 vs. 0.27; p<0.001). In children whose S. mutans level was scored as 0 to 3 by the culture-based assay, the mean caries increments were 0.31, 0.42, 1.00, and 1.56, respectively; significant differences existed only between some groups (score 0 and 2/3; score 1 and 3; all p<0.05). The sensitivity/specificity of the immunoassay, culture-bases assay, and 'past caries' in predicting ECC were 0.70/0.92, 0.70/0.74, and 0.70/0.83, respectively. Conclusions: The novel immunoassay appeared to be more specific than the culture-based assay and 'past caries' in predicting ECC and may potentially enhance multifactorial caries prediction (Supported by HHSRF#07080741 and CRCG#200907176096). |
Description | Poster Presentation Session 299: Salivary Diagnostics 1 |
Persistent Identifier | http://hdl.handle.net/10722/183216 |
ISSN | 2023 Impact Factor: 5.7 2023 SCImago Journal Rankings: 1.909 |
DC Field | Value | Language |
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dc.contributor.author | Gao, X | en_US |
dc.contributor.author | Seneviratne, CJ | en_US |
dc.contributor.author | Lo, ECM | en_US |
dc.contributor.author | Chu, CH | en_US |
dc.contributor.author | Samaranayake, LP | en_US |
dc.date.accessioned | 2013-05-15T01:48:18Z | - |
dc.date.available | 2013-05-15T01:48:18Z | - |
dc.date.issued | 2013 | en_US |
dc.identifier.citation | The 91st General Session & Exhibition of the International Association for Dental Research (IADR), Seattle, Washington, USA, 20-23 March 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue A: abstract no. 2604 | en_US |
dc.identifier.issn | 0022-0345 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/183216 | - |
dc.description | Poster Presentation | - |
dc.description | Session 299: Salivary Diagnostics 1 | - |
dc.description.abstract | Objectives: Abundance of S. mutans in saliva is an important microbiological parameter for assessing caries risk. A novel monoclonal antibody-based immunoassay (Saliva-Check mutans) was developed and outperformed the conventional culture-based assay (Dentocult SM) in enumerating S. mutans in saliva. This study aimed to evaluate and compare their validity in predicting early childhood caries (ECC). Methods: With ethical approval and parental written consent, 190 preschool children aged 3-4 years were recruited. The abundance of S. mutans in their stimulated saliva samples was classified by the culture-based assay into four levels (0-3), while the immunoassay categorized the samples into two groups with high and low S. mutans levels. Children's tooth status was assessed at baseline and after 12 months. Caries increment (△dmft) was recorded. The validity of both assays in predicting new caries (△dmft>0) was evaluated and benchmarked against that of 'past caries', which is regarded as the strongest indicator for future caries. Results: The mean caries increment was higher in children with 'past caries' than in those caries-free at baseline (1.46 vs. 0.25; p<0.001). Compared with children classified by the immunoassay as harboring low level of S. mutans, children with high level of S. mutans developed more new caries (1.66 vs. 0.27; p<0.001). In children whose S. mutans level was scored as 0 to 3 by the culture-based assay, the mean caries increments were 0.31, 0.42, 1.00, and 1.56, respectively; significant differences existed only between some groups (score 0 and 2/3; score 1 and 3; all p<0.05). The sensitivity/specificity of the immunoassay, culture-bases assay, and 'past caries' in predicting ECC were 0.70/0.92, 0.70/0.74, and 0.70/0.83, respectively. Conclusions: The novel immunoassay appeared to be more specific than the culture-based assay and 'past caries' in predicting ECC and may potentially enhance multifactorial caries prediction (Supported by HHSRF#07080741 and CRCG#200907176096). | - |
dc.language | eng | en_US |
dc.publisher | Sage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925 | en_US |
dc.relation.ispartof | Journal of Dental Research | en_US |
dc.rights | Journal of Dental Research. Copyright © Sage Publications, Inc. | en_US |
dc.subject | Caries | - |
dc.subject | Children | - |
dc.subject | Immunology | - |
dc.subject | S. mutans and Saliva | - |
dc.title | Novel S. Mutans Detection System in Predicting Early Childhood Caries | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Gao, X: gaoxl@hkucc.hku.hk | en_US |
dc.identifier.email | Seneviratne, CJ: jaya@hku.hk | en_US |
dc.identifier.email | Lo, ECM: hrdplcm@hkucc.hku.hk | en_US |
dc.identifier.email | Chu, CH: chchu@hku.hk | en_US |
dc.identifier.email | Samaranayake, LP: lakshman@hku.hk | en_US |
dc.identifier.authority | Gao, X=rp01509 | en_US |
dc.identifier.authority | Seneviratne, CJ=rp01372 | en_US |
dc.identifier.authority | Lo, ECM=rp00015 | en_US |
dc.identifier.authority | Chu, CH=rp00022 | en_US |
dc.identifier.authority | Samaranayake, LP=rp00023 | en_US |
dc.identifier.hkuros | 214395 | en_US |
dc.identifier.volume | 92 | en_US |
dc.identifier.issue | Special Issue A: abstract no. 2604 | en_US |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0022-0345 | - |