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Conference Paper: Novel S. Mutans Detection System in Predicting Early Childhood Caries

TitleNovel S. Mutans Detection System in Predicting Early Childhood Caries
Authors
KeywordsCaries
Children
Immunology
S. mutans and Saliva
Issue Date2013
PublisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The 91st General Session & Exhibition of the International Association for Dental Research (IADR), Seattle, Washington, USA, 20-23 March 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue A: abstract no. 2604 How to Cite?
AbstractObjectives: Abundance of S. mutans in saliva is an important microbiological parameter for assessing caries risk. A novel monoclonal antibody-based immunoassay (Saliva-Check mutans) was developed and outperformed the conventional culture-based assay (Dentocult SM) in enumerating S. mutans in saliva. This study aimed to evaluate and compare their validity in predicting early childhood caries (ECC). Methods: With ethical approval and parental written consent, 190 preschool children aged 3-4 years were recruited. The abundance of S. mutans in their stimulated saliva samples was classified by the culture-based assay into four levels (0-3), while the immunoassay categorized the samples into two groups with high and low S. mutans levels. Children's tooth status was assessed at baseline and after 12 months. Caries increment (△dmft) was recorded. The validity of both assays in predicting new caries (△dmft>0) was evaluated and benchmarked against that of 'past caries', which is regarded as the strongest indicator for future caries. Results: The mean caries increment was higher in children with 'past caries' than in those caries-free at baseline (1.46 vs. 0.25; p<0.001). Compared with children classified by the immunoassay as harboring low level of S. mutans, children with high level of S. mutans developed more new caries (1.66 vs. 0.27; p<0.001). In children whose S. mutans level was scored as 0 to 3 by the culture-based assay, the mean caries increments were 0.31, 0.42, 1.00, and 1.56, respectively; significant differences existed only between some groups (score 0 and 2/3; score 1 and 3; all p<0.05). The sensitivity/specificity of the immunoassay, culture-bases assay, and 'past caries' in predicting ECC were 0.70/0.92, 0.70/0.74, and 0.70/0.83, respectively. Conclusions: The novel immunoassay appeared to be more specific than the culture-based assay and 'past caries' in predicting ECC and may potentially enhance multifactorial caries prediction (Supported by HHSRF#07080741 and CRCG#200907176096).
DescriptionPoster Presentation
Session 299: Salivary Diagnostics 1
Persistent Identifierhttp://hdl.handle.net/10722/183216
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorGao, Xen_US
dc.contributor.authorSeneviratne, CJen_US
dc.contributor.authorLo, ECMen_US
dc.contributor.authorChu, CHen_US
dc.contributor.authorSamaranayake, LPen_US
dc.date.accessioned2013-05-15T01:48:18Z-
dc.date.available2013-05-15T01:48:18Z-
dc.date.issued2013en_US
dc.identifier.citationThe 91st General Session & Exhibition of the International Association for Dental Research (IADR), Seattle, Washington, USA, 20-23 March 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue A: abstract no. 2604en_US
dc.identifier.issn0022-0345en_US
dc.identifier.urihttp://hdl.handle.net/10722/183216-
dc.descriptionPoster Presentation-
dc.descriptionSession 299: Salivary Diagnostics 1-
dc.description.abstractObjectives: Abundance of S. mutans in saliva is an important microbiological parameter for assessing caries risk. A novel monoclonal antibody-based immunoassay (Saliva-Check mutans) was developed and outperformed the conventional culture-based assay (Dentocult SM) in enumerating S. mutans in saliva. This study aimed to evaluate and compare their validity in predicting early childhood caries (ECC). Methods: With ethical approval and parental written consent, 190 preschool children aged 3-4 years were recruited. The abundance of S. mutans in their stimulated saliva samples was classified by the culture-based assay into four levels (0-3), while the immunoassay categorized the samples into two groups with high and low S. mutans levels. Children's tooth status was assessed at baseline and after 12 months. Caries increment (△dmft) was recorded. The validity of both assays in predicting new caries (△dmft>0) was evaluated and benchmarked against that of 'past caries', which is regarded as the strongest indicator for future caries. Results: The mean caries increment was higher in children with 'past caries' than in those caries-free at baseline (1.46 vs. 0.25; p<0.001). Compared with children classified by the immunoassay as harboring low level of S. mutans, children with high level of S. mutans developed more new caries (1.66 vs. 0.27; p<0.001). In children whose S. mutans level was scored as 0 to 3 by the culture-based assay, the mean caries increments were 0.31, 0.42, 1.00, and 1.56, respectively; significant differences existed only between some groups (score 0 and 2/3; score 1 and 3; all p<0.05). The sensitivity/specificity of the immunoassay, culture-bases assay, and 'past caries' in predicting ECC were 0.70/0.92, 0.70/0.74, and 0.70/0.83, respectively. Conclusions: The novel immunoassay appeared to be more specific than the culture-based assay and 'past caries' in predicting ECC and may potentially enhance multifactorial caries prediction (Supported by HHSRF#07080741 and CRCG#200907176096).-
dc.languageengen_US
dc.publisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925en_US
dc.relation.ispartofJournal of Dental Researchen_US
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.en_US
dc.subjectCaries-
dc.subjectChildren-
dc.subjectImmunology-
dc.subjectS. mutans and Saliva-
dc.titleNovel S. Mutans Detection System in Predicting Early Childhood Cariesen_US
dc.typeConference_Paperen_US
dc.identifier.emailGao, X: gaoxl@hkucc.hku.hken_US
dc.identifier.emailSeneviratne, CJ: jaya@hku.hken_US
dc.identifier.emailLo, ECM: hrdplcm@hkucc.hku.hken_US
dc.identifier.emailChu, CH: chchu@hku.hken_US
dc.identifier.emailSamaranayake, LP: lakshman@hku.hken_US
dc.identifier.authorityGao, X=rp01509en_US
dc.identifier.authoritySeneviratne, CJ=rp01372en_US
dc.identifier.authorityLo, ECM=rp00015en_US
dc.identifier.authorityChu, CH=rp00022en_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.identifier.hkuros214395en_US
dc.identifier.volume92en_US
dc.identifier.issueSpecial Issue A: abstract no. 2604en_US
dc.publisher.placeUnited States-

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