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Conference Paper: Developing a hydrogel micro-environment for biomimetic mineralization of enamel

TitleDeveloping a hydrogel micro-environment for biomimetic mineralization of enamel
Authors
KeywordsBiomaterials
Enamel
Fluoride
Remineralization and hydrogel
Issue Date2013
PublisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The 91st General Session & Exhibition of the International Association for Dental Research (IADR), Seattle, Washington, USA, 20-23 March 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue A: abstract no. 2548 How to Cite?
AbstractObjective: To describe the effects of a novel hydrogel biomimetic mineralization micro-environment in initiating growth of enamel prisms-like crystals on human enamel surface. Method: Enamel slices were prepared from extracted human third molars. The outer enamel surface was polished smooth and etched with a 37% phosphoric acid. The etched enamel surface was covered by a layer of 2mm thick agarose gel containing calcium chloride. On top of the agarose gel layer, there is another layer of 2mm thick calcium ion free agarose gel. The slices, together with the agarose gel, were immersed into a freshly prepared and acidity neutral solution containing sodium dihydrogen phosphate and sodium fluoride, and incubated at 37oC for 10 days. The solution was replaced every 24 hours and the agarose gel was replaced every 48 hours. Scanning electron microscope (SEM) was used to study the crystals precipitated on enamel surface. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) were used to evaluate the chemical structure of the crystals. Result: Observation under SEM showed crystals were precipitated and covered the enamel surface. Higher magnification (50,000x) showed that the crystals had enamel prismatic structure with the size of about 1 micrometer. They were orderly formed in bundles parallel to each other. XRD and FTIR analysis suggested that the crystals were hydroxyapatite. Conclusion: In this study, the novel hydrogel acts as biomimetic mineralization micro-environment to initiate growth of enamel prisms-like crystals on human enamel surface.
DescriptionPoster Presentation ;(2) Session 293: De- and Remineralization
Session 293: De- and Remineralization
Persistent Identifierhttp://hdl.handle.net/10722/183215
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorCao, Yen_US
dc.contributor.authorLi, Qen_US
dc.contributor.authorChu, CHen_US
dc.contributor.authorLo, ECMen_US
dc.date.accessioned2013-05-15T01:48:18Z-
dc.date.available2013-05-15T01:48:18Z-
dc.date.issued2013en_US
dc.identifier.citationThe 91st General Session & Exhibition of the International Association for Dental Research (IADR), Seattle, Washington, USA, 20-23 March 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue A: abstract no. 2548en_US
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/183215-
dc.descriptionPoster Presentation ;(2) Session 293: De- and Remineralization-
dc.descriptionSession 293: De- and Remineralization-
dc.description.abstractObjective: To describe the effects of a novel hydrogel biomimetic mineralization micro-environment in initiating growth of enamel prisms-like crystals on human enamel surface. Method: Enamel slices were prepared from extracted human third molars. The outer enamel surface was polished smooth and etched with a 37% phosphoric acid. The etched enamel surface was covered by a layer of 2mm thick agarose gel containing calcium chloride. On top of the agarose gel layer, there is another layer of 2mm thick calcium ion free agarose gel. The slices, together with the agarose gel, were immersed into a freshly prepared and acidity neutral solution containing sodium dihydrogen phosphate and sodium fluoride, and incubated at 37oC for 10 days. The solution was replaced every 24 hours and the agarose gel was replaced every 48 hours. Scanning electron microscope (SEM) was used to study the crystals precipitated on enamel surface. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) were used to evaluate the chemical structure of the crystals. Result: Observation under SEM showed crystals were precipitated and covered the enamel surface. Higher magnification (50,000x) showed that the crystals had enamel prismatic structure with the size of about 1 micrometer. They were orderly formed in bundles parallel to each other. XRD and FTIR analysis suggested that the crystals were hydroxyapatite. Conclusion: In this study, the novel hydrogel acts as biomimetic mineralization micro-environment to initiate growth of enamel prisms-like crystals on human enamel surface.-
dc.languageengen_US
dc.publisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925-
dc.relation.ispartofJournal of Dental Researchen_US
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.-
dc.subjectBiomaterials-
dc.subjectEnamel-
dc.subjectFluoride-
dc.subjectRemineralization and hydrogel-
dc.titleDeveloping a hydrogel micro-environment for biomimetic mineralization of enamelen_US
dc.typeConference_Paperen_US
dc.identifier.emailChu, CH: chchu@hku.hken_US
dc.identifier.emailLo, ECM: hrdplcm@hkucc.hku.hken_US
dc.identifier.authorityChu, CH=rp00022en_US
dc.identifier.authorityLo, ECM=rp00015en_US
dc.identifier.hkuros214394en_US
dc.identifier.volume92en_US
dc.identifier.issueSpecial Issue A: abstract no. 2548en_US
dc.publisher.placeUnited States-

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