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Conference Paper: Laser effects on dentine treated with silver diamine fluoride

TitleLaser effects on dentine treated with silver diamine fluoride
Authors
KeywordsBiofilm
Caries
Cariology
Dentin and Lasers
Issue Date2012
PublisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The Annual Meeting of the International Association for Dental Research (IADR) Southeast Asian Division, Hong Kong, China, 3-4 November 2012. In Journal of Dental Research, 2012, v. 91 n. Special Issue C: abstract no. 168999 How to Cite?
AbstractObjectives: To evaluate the effect of Er:YAG (2,940nm) Laser irradiation and 38% silver diamine fluoride (SDF) application on human sound dentine under cariogenic biofilm challenge. Methods: Six 3mm thick dentine slices were prepared from 6 sound human molars. Each slice was divided into 4 areas for application of distilled water as control (Area 1). Er:YAG Laser (Area 2), SDF (Area 3) and SDF plus Er:YAG Laser (Area 4). The laser irradiation was set at a 100µs pulse and 20Hz repetition rate and applied for 1 sec/mm2. The treated dentine slices were subjected to biofilm challenge, which was formed by five cariogenic bacterias, namely, Actinomyces naeslundii, Lactobacillus acidophilus, Lactobacillus rhamnosus, Streptococcus mutans, Streptococcus sobrinus, in 5% sucrose solution at 37oC for 12 hours. Finally, they were immersed in a buffered remineralizing solution containing calcium chloride and sodium hypophosphate for 12 hours. The surface morphology of the 4 areas of each slice was examined using Scanning Electronic Microscopy. Energy Dispersive X-ray Spectrometery (EDX) was used for elemental analysis. Results: Smooth dentine surface was observed on Areas 1 and 3. A scaly surface with craterlike structures of the dentine surface was observed in Areas 2 and 4. The % weight of fluoride in Areas 1 to 4 were 7.8%, 8.0%, 8.4% and 9.8%, respectively. Area 4 had a higher % weight of fluoride than the other 3 Areas (p=0.029). The Ca/P ratios of Areas 1 to 4 were 1.89, 2.07, 1.92 and 2.40, respectively. The Ca/P ratio in Area 4 was higher than the other 3 Areas (p=0.018). Conclusions: In this laboratory study, SDF application combined with Er:YAG laser irradiation on dentine increased the fluoride content and diminished the demineralization induced by the cariogenic biofilm. This study is supported by HKU Seed Funding Programme for Basic Research #201111159079
DescriptionSession: Cariology Research
Persistent Identifierhttp://hdl.handle.net/10722/182070
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorIto, Len_US
dc.contributor.authorMei, Len_US
dc.contributor.authorZhang, Cen_US
dc.contributor.authorLo, ECMen_US
dc.contributor.authorChu, CHen_US
dc.date.accessioned2013-04-17T07:20:46Z-
dc.date.available2013-04-17T07:20:46Z-
dc.date.issued2012en_US
dc.identifier.citationThe Annual Meeting of the International Association for Dental Research (IADR) Southeast Asian Division, Hong Kong, China, 3-4 November 2012. In Journal of Dental Research, 2012, v. 91 n. Special Issue C: abstract no. 168999en_US
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/182070-
dc.descriptionSession: Cariology Research-
dc.description.abstractObjectives: To evaluate the effect of Er:YAG (2,940nm) Laser irradiation and 38% silver diamine fluoride (SDF) application on human sound dentine under cariogenic biofilm challenge. Methods: Six 3mm thick dentine slices were prepared from 6 sound human molars. Each slice was divided into 4 areas for application of distilled water as control (Area 1). Er:YAG Laser (Area 2), SDF (Area 3) and SDF plus Er:YAG Laser (Area 4). The laser irradiation was set at a 100µs pulse and 20Hz repetition rate and applied for 1 sec/mm2. The treated dentine slices were subjected to biofilm challenge, which was formed by five cariogenic bacterias, namely, Actinomyces naeslundii, Lactobacillus acidophilus, Lactobacillus rhamnosus, Streptococcus mutans, Streptococcus sobrinus, in 5% sucrose solution at 37oC for 12 hours. Finally, they were immersed in a buffered remineralizing solution containing calcium chloride and sodium hypophosphate for 12 hours. The surface morphology of the 4 areas of each slice was examined using Scanning Electronic Microscopy. Energy Dispersive X-ray Spectrometery (EDX) was used for elemental analysis. Results: Smooth dentine surface was observed on Areas 1 and 3. A scaly surface with craterlike structures of the dentine surface was observed in Areas 2 and 4. The % weight of fluoride in Areas 1 to 4 were 7.8%, 8.0%, 8.4% and 9.8%, respectively. Area 4 had a higher % weight of fluoride than the other 3 Areas (p=0.029). The Ca/P ratios of Areas 1 to 4 were 1.89, 2.07, 1.92 and 2.40, respectively. The Ca/P ratio in Area 4 was higher than the other 3 Areas (p=0.018). Conclusions: In this laboratory study, SDF application combined with Er:YAG laser irradiation on dentine increased the fluoride content and diminished the demineralization induced by the cariogenic biofilm. This study is supported by HKU Seed Funding Programme for Basic Research #201111159079-
dc.languageengen_US
dc.publisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925-
dc.relation.ispartofJournal of Dental Researchen_US
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.-
dc.subjectBiofilm-
dc.subjectCaries-
dc.subjectCariology-
dc.subjectDentin and Lasers-
dc.titleLaser effects on dentine treated with silver diamine fluorideen_US
dc.typeConference_Paperen_US
dc.identifier.emailMei, L: leimei@hkusua.hku.hken_US
dc.identifier.emailZhang, C: zhangcf@hku.hken_US
dc.identifier.emailLo, ECM: hrdplcm@hkucc.hku.hken_US
dc.identifier.emailChu, CH: chchu@hku.hken_US
dc.identifier.authorityMei, L=rp01840en_US
dc.identifier.authorityZhang, C=rp01408en_US
dc.identifier.authorityLo, ECM=rp00015en_US
dc.identifier.hkuros213932en_US
dc.identifier.volume91en_US
dc.identifier.issueSpecial Issue C: abstract no. 168999en_US
dc.publisher.placeUnited States-

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