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Article: Gene expression studies of the dikaryotic mycelium and primordium of Lentinula edodes by serial analysis of gene expression

TitleGene expression studies of the dikaryotic mycelium and primordium of Lentinula edodes by serial analysis of gene expression
Authors
Issue Date2008
PublisherElsevier Ltd.
Citation
Mycological Research, 2008, v. 112 n. 8, p. 950-964 How to Cite?
AbstractLentinula edodes (Shiitake mushroom) is a common edible mushroom that has high nutritional and medical value. Although a number of genes involved in the fruit of the species have been identified, little is known about the process of differentiation from dikaryotic mycelium to primordium. In this study, serial analysis of gene expression (SAGE) was applied to determine the gene expression profiles of the dikaryotic mycelium and primordium of L. edodes in an effort to advance our understanding of the molecular basis of fruit body development. A total of 6363 tags were extracted (3278 from the dikaryotic mycelium and 3085 from the primordium), 164 unique tags matched the in-house expressed sequence tag (EST) database. The difference between the expression profiles of the dikaryotic mycelium and primordium suggests that a specific set of genes is required for fruit body development. In the transition from the mycelium to the primordium, different hydrophobins were expressed abundantly, fewer structural genes were expressed, transcription and translation became active, different genes became involved in intracellular trafficking, and stress responses were expressed. These findings advance our understanding of fruit body development. We used cDNA microarray hybridization and Northern blotting to verify the SAGE results, and found SAGE to be highly efficient in the performance of transcriptome analysis. To our knowledge, this is the first SAGE study of a mushroom.
Persistent Identifierhttp://hdl.handle.net/10722/181274
ISSN
2011 Impact Factor: 2.809
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChum, WWY-
dc.contributor.authorNg, TP-
dc.contributor.authorShih, RSM-
dc.contributor.authorAu, CH-
dc.contributor.authorKwan, HS-
dc.date.accessioned2013-02-21T04:07:54Z-
dc.date.available2013-02-21T04:07:54Z-
dc.date.issued2008-
dc.identifier.citationMycological Research, 2008, v. 112 n. 8, p. 950-964-
dc.identifier.issn0953-7562-
dc.identifier.urihttp://hdl.handle.net/10722/181274-
dc.description.abstractLentinula edodes (Shiitake mushroom) is a common edible mushroom that has high nutritional and medical value. Although a number of genes involved in the fruit of the species have been identified, little is known about the process of differentiation from dikaryotic mycelium to primordium. In this study, serial analysis of gene expression (SAGE) was applied to determine the gene expression profiles of the dikaryotic mycelium and primordium of L. edodes in an effort to advance our understanding of the molecular basis of fruit body development. A total of 6363 tags were extracted (3278 from the dikaryotic mycelium and 3085 from the primordium), 164 unique tags matched the in-house expressed sequence tag (EST) database. The difference between the expression profiles of the dikaryotic mycelium and primordium suggests that a specific set of genes is required for fruit body development. In the transition from the mycelium to the primordium, different hydrophobins were expressed abundantly, fewer structural genes were expressed, transcription and translation became active, different genes became involved in intracellular trafficking, and stress responses were expressed. These findings advance our understanding of fruit body development. We used cDNA microarray hybridization and Northern blotting to verify the SAGE results, and found SAGE to be highly efficient in the performance of transcriptome analysis. To our knowledge, this is the first SAGE study of a mushroom.-
dc.languageeng-
dc.publisherElsevier Ltd.-
dc.relation.ispartofMycological Research-
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Mycological Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Mycological Research, [VOL 112, ISSUE 8, 2008] DOI 10.1016/j.mycres.2008.01.028-
dc.subject.meshFruiting Bodies, Fungal - genetics - growth and development - metabolism-
dc.subject.meshGene Expression Profiling-
dc.subject.meshGene Expression Regulation, Developmental-
dc.subject.meshOligonucleotide Array Sequence Analysis-
dc.subject.meshShiitake Mushrooms - genetics - growth and development - metabolism-
dc.titleGene expression studies of the dikaryotic mycelium and primordium of Lentinula edodes by serial analysis of gene expressionen_US
dc.typeArticleen_US
dc.identifier.emailNg, TP: ledodes@hkucc.hku.hk-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.mycres.2008.01.028-
dc.identifier.pmid18555678-
dc.identifier.scopuseid_2-s2.0-47149112357-
dc.identifier.volume112-
dc.identifier.issue8-
dc.identifier.spage950-
dc.identifier.epage964-
dc.identifier.isiWOS:000258809700007-
dc.publisher.placeUnited Kingdom-

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