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Article: Overexpression of a novel endogenous NADH kinase in Aspergillus nidulans enhances growth

TitleOverexpression of a novel endogenous NADH kinase in Aspergillus nidulans enhances growth
Authors
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/meteng
Citation
Metabolic Engineering, 2009, v. 11 n. 1, p. 31-39 How to Cite?
AbstractThe complete genome sequence of the filamentous fungi Aspergillus nidulans has paved the way for fundamental research on this industrially important species. To the best of our knowledge, this is the first time a gene encoding for ATP-dependent NADH kinase (ATP:NADH 2′-phosphotransferase, EC 2.7.1.86) has been identified. The enzyme has a predicted molecular weight of 49 kDa. We characterised the role of this NADH kinase by genomic integration of the putative gene AN8837.2 under a strong constitutive promoter. The physiological effects of overexpressed NADH kinase in combination with different aeration rates were studied in well-controlled glucose batch fermentations. Metabolite profiling and metabolic network analysis with [1-13C] glucose were used for characterisation of the strains, and the results demonstrated that NADH kinase activity has paramount influence on growth physiology. Biomass yield on glucose and the maximum specific growth rate increased from 0.47 g/g and 0.22 h-1 (wild type) to 0.54 g/g and 0.26 h-1 (NADH kinase overexpressed), respectively. The results suggest that overexpression of NADH kinase improves the growth efficiency of the cell by increasing the access to NADPH. Our findings indicate that A. nidulans is not optimised for growth in nutrient-rich conditions typically found in laboratory and industrial fermentors. This conclusion may impact the design of new strains capable of generating reducing power in the form of NADPH, which is crucial for efficient production of many industrially important metabolites and enzymes. © 2008 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/181252
ISSN
2015 Impact Factor: 8.201
2015 SCImago Journal Rankings: 3.655
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPanagiotou, Gen_US
dc.contributor.authorGrotkjær, Ten_US
dc.contributor.authorHofmann, Gen_US
dc.contributor.authorBapat, PMen_US
dc.contributor.authorOlsson, Len_US
dc.date.accessioned2013-02-21T02:03:30Z-
dc.date.available2013-02-21T02:03:30Z-
dc.date.issued2009en_US
dc.identifier.citationMetabolic Engineering, 2009, v. 11 n. 1, p. 31-39en_US
dc.identifier.issn1096-7176en_US
dc.identifier.urihttp://hdl.handle.net/10722/181252-
dc.description.abstractThe complete genome sequence of the filamentous fungi Aspergillus nidulans has paved the way for fundamental research on this industrially important species. To the best of our knowledge, this is the first time a gene encoding for ATP-dependent NADH kinase (ATP:NADH 2′-phosphotransferase, EC 2.7.1.86) has been identified. The enzyme has a predicted molecular weight of 49 kDa. We characterised the role of this NADH kinase by genomic integration of the putative gene AN8837.2 under a strong constitutive promoter. The physiological effects of overexpressed NADH kinase in combination with different aeration rates were studied in well-controlled glucose batch fermentations. Metabolite profiling and metabolic network analysis with [1-13C] glucose were used for characterisation of the strains, and the results demonstrated that NADH kinase activity has paramount influence on growth physiology. Biomass yield on glucose and the maximum specific growth rate increased from 0.47 g/g and 0.22 h-1 (wild type) to 0.54 g/g and 0.26 h-1 (NADH kinase overexpressed), respectively. The results suggest that overexpression of NADH kinase improves the growth efficiency of the cell by increasing the access to NADPH. Our findings indicate that A. nidulans is not optimised for growth in nutrient-rich conditions typically found in laboratory and industrial fermentors. This conclusion may impact the design of new strains capable of generating reducing power in the form of NADPH, which is crucial for efficient production of many industrially important metabolites and enzymes. © 2008 Elsevier Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/metengen_US
dc.relation.ispartofMetabolic Engineeringen_US
dc.subject.meshAspergillus Nidulans - Enzymology - Growth & Developmenten_US
dc.subject.meshFungal Proteins - Genetics - Metabolismen_US
dc.subject.meshPhosphotransferases - Genetics - Metabolismen_US
dc.subject.meshPhylogenyen_US
dc.titleOverexpression of a novel endogenous NADH kinase in Aspergillus nidulans enhances growthen_US
dc.typeArticleen_US
dc.identifier.emailPanagiotou, G: gipa@hku.hken_US
dc.identifier.authorityPanagiotou, G=rp01725en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.ymben.2008.08.008en_US
dc.identifier.pmid18840540-
dc.identifier.scopuseid_2-s2.0-55549092023en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-55549092023&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume11en_US
dc.identifier.issue1en_US
dc.identifier.spage31en_US
dc.identifier.epage39en_US
dc.identifier.isiWOS:000262502800005-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridPanagiotou, G=8566179700en_US
dc.identifier.scopusauthoridGrotkjær, T=6506918583en_US
dc.identifier.scopusauthoridHofmann, G=7202274378en_US
dc.identifier.scopusauthoridBapat, PM=7004498641en_US
dc.identifier.scopusauthoridOlsson, L=7203077540en_US

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