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Article: Identification of NADH kinase activity in filamentous fungi and structural modelling of the novel enzyme from Fusarium oxysporum

TitleIdentification of NADH kinase activity in filamentous fungi and structural modelling of the novel enzyme from Fusarium oxysporum
Authors
KeywordsFungal Nadh Kinase
Fusarium Oxysporum
Purification
Structural Modelling
Issue Date2008
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/procbio
Citation
Process Biochemistry, 2008, v. 43 n. 10, p. 1114-1120 How to Cite?
AbstractATP-NADH kinase phosphorylates NADH to produce NADPH at the expense of ATP. The present study describes Fusarium oxysporum NADH kinase (ATP:NADH 2′-phosphotransferase, EC 2.7.1.86), a novel fungal enzyme capable of synthesizing NADPH using NADH as the preferred diphosphonicotinamide (diphosphopyridine) nucleotide donor. NADH kinase was highly purified (∼66-fold) and the enzyme was found to be a homodimeric with a subunit of M r 72,000. Isoelectric focusing in the pH range of 3.0-9.5 of the purified NADH kinase yielded a pI value of about 5.6. The K m values of NADH kinase for NADH and ATP were found to be 0.13 and 2.59 mM, respectively. Prediction of the secondary structure of the protein was performed in the PSIPRED server while modelling the three-dimensional (3D) structure was accomplished by the use of the HH 3D-structure prediction server. © 2008 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/181251
ISSN
2015 Impact Factor: 2.529
2015 SCImago Journal Rankings: 0.937
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPanagiotou, Gen_US
dc.contributor.authorPapadakis, MAen_US
dc.contributor.authorTopakas, Een_US
dc.contributor.authorOlsson, Len_US
dc.contributor.authorChristakopoulos, Pen_US
dc.date.accessioned2013-02-21T02:03:30Z-
dc.date.available2013-02-21T02:03:30Z-
dc.date.issued2008en_US
dc.identifier.citationProcess Biochemistry, 2008, v. 43 n. 10, p. 1114-1120en_US
dc.identifier.issn1359-5113en_US
dc.identifier.urihttp://hdl.handle.net/10722/181251-
dc.description.abstractATP-NADH kinase phosphorylates NADH to produce NADPH at the expense of ATP. The present study describes Fusarium oxysporum NADH kinase (ATP:NADH 2′-phosphotransferase, EC 2.7.1.86), a novel fungal enzyme capable of synthesizing NADPH using NADH as the preferred diphosphonicotinamide (diphosphopyridine) nucleotide donor. NADH kinase was highly purified (∼66-fold) and the enzyme was found to be a homodimeric with a subunit of M r 72,000. Isoelectric focusing in the pH range of 3.0-9.5 of the purified NADH kinase yielded a pI value of about 5.6. The K m values of NADH kinase for NADH and ATP were found to be 0.13 and 2.59 mM, respectively. Prediction of the secondary structure of the protein was performed in the PSIPRED server while modelling the three-dimensional (3D) structure was accomplished by the use of the HH 3D-structure prediction server. © 2008 Elsevier Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/procbioen_US
dc.relation.ispartofProcess Biochemistryen_US
dc.subjectFungal Nadh Kinaseen_US
dc.subjectFusarium Oxysporumen_US
dc.subjectPurificationen_US
dc.subjectStructural Modellingen_US
dc.titleIdentification of NADH kinase activity in filamentous fungi and structural modelling of the novel enzyme from Fusarium oxysporumen_US
dc.typeArticleen_US
dc.identifier.emailPanagiotou, G: gipa@hku.hken_US
dc.identifier.authorityPanagiotou, G=rp01725en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.procbio.2008.06.011en_US
dc.identifier.scopuseid_2-s2.0-50849087683en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-50849087683&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume43en_US
dc.identifier.issue10en_US
dc.identifier.spage1114en_US
dc.identifier.epage1120en_US
dc.identifier.isiWOS:000259773100014-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridPanagiotou, G=8566179700en_US
dc.identifier.scopusauthoridPapadakis, MA=24475503600en_US
dc.identifier.scopusauthoridTopakas, E=6603178486en_US
dc.identifier.scopusauthoridOlsson, L=7203077540en_US
dc.identifier.scopusauthoridChristakopoulos, P=7006479823en_US

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