File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: A trispecies Aspergillus microarray: Comparative transcriptomics of three Aspergillus species

TitleA trispecies Aspergillus microarray: Comparative transcriptomics of three Aspergillus species
Authors
KeywordsAspergillus Nidulans
Aspergillus Niger
Aspergillus Oryzae
Xlnr
Issue Date2008
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings Of The National Academy Of Sciences Of The United States Of America, 2008, v. 105 n. 11, p. 4387-4392 How to Cite?
AbstractThe full-genome sequencing of the filamentous fungi Aspergillus nidulans, Aspergillus niger, and Aspergillus oryzae has opened possibilities for studying the cellular physiology of these fungi on a systemic level. As a tool to explore this, we are making available an Affymetrix GeneChip developed for transcriptome analysis of any of the three above-mentioned aspergilli. Transcriptome analysis of triplicate batch cultivations of all three aspergilli on glucose and xylose media was used to validate the performance of the microarray. Gene comparisons of all three species and cross-analysis with the expression data identified 23 genes to be a conserved response across Aspergillus sp., including the xylose transcriptional activator XlnR. A promoter analysis of the upregulated genes in all three species indicates the conserved XlnR-binding site to be 5′-GGNTAAA-3′. The composition of the conserved gene-set suggests that xylose acts as a molecule, indicating the presence of complex carbohydrates such as hemicellulose, and triggers an array of degrading enzymes. With this case example, we present a validated tool for transcriptome analysis of three Aspergillus species and a methodology for conducting cross-species evolutionary studies within a genus using comparative transcriptomics. © 2008 by The National Academy of Sciences of the USA.
Persistent Identifierhttp://hdl.handle.net/10722/181249
ISSN
2015 Impact Factor: 9.423
2015 SCImago Journal Rankings: 6.883
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorAndersen, MRen_US
dc.contributor.authorVongsangnak, Wen_US
dc.contributor.authorPanagiotou, Gen_US
dc.contributor.authorSalazar, MPen_US
dc.contributor.authorLehmann, Len_US
dc.contributor.authorNielsen, Jen_US
dc.date.accessioned2013-02-21T02:03:29Z-
dc.date.available2013-02-21T02:03:29Z-
dc.date.issued2008en_US
dc.identifier.citationProceedings Of The National Academy Of Sciences Of The United States Of America, 2008, v. 105 n. 11, p. 4387-4392en_US
dc.identifier.issn0027-8424en_US
dc.identifier.urihttp://hdl.handle.net/10722/181249-
dc.description.abstractThe full-genome sequencing of the filamentous fungi Aspergillus nidulans, Aspergillus niger, and Aspergillus oryzae has opened possibilities for studying the cellular physiology of these fungi on a systemic level. As a tool to explore this, we are making available an Affymetrix GeneChip developed for transcriptome analysis of any of the three above-mentioned aspergilli. Transcriptome analysis of triplicate batch cultivations of all three aspergilli on glucose and xylose media was used to validate the performance of the microarray. Gene comparisons of all three species and cross-analysis with the expression data identified 23 genes to be a conserved response across Aspergillus sp., including the xylose transcriptional activator XlnR. A promoter analysis of the upregulated genes in all three species indicates the conserved XlnR-binding site to be 5′-GGNTAAA-3′. The composition of the conserved gene-set suggests that xylose acts as a molecule, indicating the presence of complex carbohydrates such as hemicellulose, and triggers an array of degrading enzymes. With this case example, we present a validated tool for transcriptome analysis of three Aspergillus species and a methodology for conducting cross-species evolutionary studies within a genus using comparative transcriptomics. © 2008 by The National Academy of Sciences of the USA.en_US
dc.languageengen_US
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.orgen_US
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen_US
dc.subjectAspergillus Nidulansen_US
dc.subjectAspergillus Nigeren_US
dc.subjectAspergillus Oryzaeen_US
dc.subjectXlnren_US
dc.titleA trispecies Aspergillus microarray: Comparative transcriptomics of three Aspergillus speciesen_US
dc.typeArticleen_US
dc.identifier.emailPanagiotou, G: gipa@hku.hken_US
dc.identifier.authorityPanagiotou, G=rp01725en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1073/pnas.0709964105en_US
dc.identifier.pmid18332432-
dc.identifier.scopuseid_2-s2.0-41949120307en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-41949120307&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume105en_US
dc.identifier.issue11en_US
dc.identifier.spage4387en_US
dc.identifier.epage4392en_US
dc.identifier.isiWOS:000254263300057-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridAndersen, MR=15841796100en_US
dc.identifier.scopusauthoridVongsangnak, W=24068171600en_US
dc.identifier.scopusauthoridPanagiotou, G=8566179700en_US
dc.identifier.scopusauthoridSalazar, MP=24067239400en_US
dc.identifier.scopusauthoridLehmann, L=24066987200en_US
dc.identifier.scopusauthoridNielsen, J=7404066338en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats