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Article: Engineering of the redox imbalance of Fusarium oxysporum enables anaerobic growth on xylose

TitleEngineering of the redox imbalance of Fusarium oxysporum enables anaerobic growth on xylose
Authors
Issue Date2006
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/meteng
Citation
Metabolic Engineering, 2006, v. 8 n. 5, p. 474-482 How to Cite?
AbstractDissimilatory nitrate reduction metabolism, of the natural xylose-fermenting fungus Fusarium oxysporum, was used as a strategy to achieve anaerobic growth and ethanol production from xylose. Beneficial alterations of the redox fluxes and thereby of the xylose metabolism were obtained by taking advantage of the regeneration of the cofactor NAD+ during the denitrification process. In batch cultivations, nitrate sustained growth under anaerobic conditions (1.21 g L-1 biomass) and simultaneously a maximum yield of 0.55 moles of ethanol per mole of xylose was achieved, whereas substitution of nitrate with ammonium limited the growth significantly (0.15 g L-1 biomass). Using nitrate, the maximum acetate yield was 0.21 moles per mole of xylose and no xylitol excretion was observed. Furthermore, the network structure in the central carbon metabolism of F. oxysporum was characterized in steady state. F. oxysporum grew anaerobically on [1-13C] labelled glucose and unlabelled xylose in chemostat cultivation with nitrate as nitrogen source. The use of labelled substrate allowed the precise determination of the glucose and xylose contribution to the carbon fluxes in the central metabolism of this poorly described microorganism. It was demonstrated that dissimilatory nitrate reduction allows F. oxysporum to exhibit typical respiratory metabolic behaviour with a highly active TCA cycle and a large demand for NADPH. © 2006 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/181241
ISSN
2015 Impact Factor: 8.201
2015 SCImago Journal Rankings: 3.655
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPanagiotou, Gen_US
dc.contributor.authorChristakopoulos, Pen_US
dc.contributor.authorGrotkjær, Ten_US
dc.contributor.authorOlsson, Len_US
dc.date.accessioned2013-02-21T02:03:26Z-
dc.date.available2013-02-21T02:03:26Z-
dc.date.issued2006en_US
dc.identifier.citationMetabolic Engineering, 2006, v. 8 n. 5, p. 474-482en_US
dc.identifier.issn1096-7176en_US
dc.identifier.urihttp://hdl.handle.net/10722/181241-
dc.description.abstractDissimilatory nitrate reduction metabolism, of the natural xylose-fermenting fungus Fusarium oxysporum, was used as a strategy to achieve anaerobic growth and ethanol production from xylose. Beneficial alterations of the redox fluxes and thereby of the xylose metabolism were obtained by taking advantage of the regeneration of the cofactor NAD+ during the denitrification process. In batch cultivations, nitrate sustained growth under anaerobic conditions (1.21 g L-1 biomass) and simultaneously a maximum yield of 0.55 moles of ethanol per mole of xylose was achieved, whereas substitution of nitrate with ammonium limited the growth significantly (0.15 g L-1 biomass). Using nitrate, the maximum acetate yield was 0.21 moles per mole of xylose and no xylitol excretion was observed. Furthermore, the network structure in the central carbon metabolism of F. oxysporum was characterized in steady state. F. oxysporum grew anaerobically on [1-13C] labelled glucose and unlabelled xylose in chemostat cultivation with nitrate as nitrogen source. The use of labelled substrate allowed the precise determination of the glucose and xylose contribution to the carbon fluxes in the central metabolism of this poorly described microorganism. It was demonstrated that dissimilatory nitrate reduction allows F. oxysporum to exhibit typical respiratory metabolic behaviour with a highly active TCA cycle and a large demand for NADPH. © 2006 Elsevier Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/metengen_US
dc.relation.ispartofMetabolic Engineeringen_US
dc.subject.meshAnaerobiosis - Physiologyen_US
dc.subject.meshBioreactors - Microbiologyen_US
dc.subject.meshCell Culture Techniques - Methodsen_US
dc.subject.meshCell Proliferationen_US
dc.subject.meshFusarium - Physiologyen_US
dc.subject.meshGenetic Enhancement - Methodsen_US
dc.subject.meshNitrates - Metabolismen_US
dc.subject.meshOxidation-Reductionen_US
dc.subject.meshXylose - Metabolismen_US
dc.titleEngineering of the redox imbalance of Fusarium oxysporum enables anaerobic growth on xyloseen_US
dc.typeArticleen_US
dc.identifier.emailPanagiotou, G: gipa@hku.hken_US
dc.identifier.authorityPanagiotou, G=rp01725en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.ymben.2006.04.004en_US
dc.identifier.pmid16797196-
dc.identifier.scopuseid_2-s2.0-33748801503en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33748801503&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume8en_US
dc.identifier.issue5en_US
dc.identifier.spage474en_US
dc.identifier.epage482en_US
dc.identifier.isiWOS:000240600800009-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridPanagiotou, G=8566179700en_US
dc.identifier.scopusauthoridChristakopoulos, P=7006479823en_US
dc.identifier.scopusauthoridGrotkjær, T=6506918583en_US
dc.identifier.scopusauthoridOlsson, L=7203077540en_US

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