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Article: Intracellular metabolite profiling of Fusarium oxysporum converting glucose to ethanol.

TitleIntracellular metabolite profiling of Fusarium oxysporum converting glucose to ethanol.
Authors
Issue Date2005
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jbiotec
Citation
Journal Of Biotechnology, 2005, v. 115 n. 4, p. 425-434 How to Cite?
AbstractThe filamentous fungus Fusarium oxysporum is known for its ability to produce ethanol by simultaneous saccharification and fermentation (SSF) of cellulose. However, the conversion rate is low and significant amounts of acetic acid are produced as a by-product. In this study, the growth characteristics of F. oxysporum were evaluated in a minimal medium using glucose as the sole carbon source in aerobic, anaerobic and oxygen-limited batch cultivations. Under aerobic conditions the maximum specific growth rate was found to be 0.043 h(-1), and the highest ethanol yield (1.66 mol/mol) was found under anaerobic conditions. During the different phases of the cultivations, the intracellular profiles were determined under aerobic and anaerobic conditions. The profiles of the phosphorylated intermediates indicated that there was a high glycolytic flux at anaerobic growth conditions, characterized by high efflux of glyceraldehyde-3-phosphate (G3P) and fructose-6-phosphate (F6P) from the pentose phosphate pathway (PPP) to the Embden-Meyerhof-Parnas (EMP) pathway, resulting in the highest ethanol production under these conditions. The amino acid profile clearly suggests that the TCA cycle was primarily active under aerobic cultivation. On the other hand, the presence of high levels of gamma-amino-n-butyric acid (GABA) under anaerobic conditions suggests a functional GABA bypass and a possible block in the TCA cycle at these conditions.
Persistent Identifierhttp://hdl.handle.net/10722/181237
ISSN
2015 Impact Factor: 2.667
2015 SCImago Journal Rankings: 1.064
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorPanagiotou, Gen_US
dc.contributor.authorVillasBôas, SGen_US
dc.contributor.authorChristakopoulos, Pen_US
dc.contributor.authorNielsen, Jen_US
dc.contributor.authorOlsson, Len_US
dc.date.accessioned2013-02-21T02:03:25Z-
dc.date.available2013-02-21T02:03:25Z-
dc.date.issued2005en_US
dc.identifier.citationJournal Of Biotechnology, 2005, v. 115 n. 4, p. 425-434en_US
dc.identifier.issn0168-1656en_US
dc.identifier.urihttp://hdl.handle.net/10722/181237-
dc.description.abstractThe filamentous fungus Fusarium oxysporum is known for its ability to produce ethanol by simultaneous saccharification and fermentation (SSF) of cellulose. However, the conversion rate is low and significant amounts of acetic acid are produced as a by-product. In this study, the growth characteristics of F. oxysporum were evaluated in a minimal medium using glucose as the sole carbon source in aerobic, anaerobic and oxygen-limited batch cultivations. Under aerobic conditions the maximum specific growth rate was found to be 0.043 h(-1), and the highest ethanol yield (1.66 mol/mol) was found under anaerobic conditions. During the different phases of the cultivations, the intracellular profiles were determined under aerobic and anaerobic conditions. The profiles of the phosphorylated intermediates indicated that there was a high glycolytic flux at anaerobic growth conditions, characterized by high efflux of glyceraldehyde-3-phosphate (G3P) and fructose-6-phosphate (F6P) from the pentose phosphate pathway (PPP) to the Embden-Meyerhof-Parnas (EMP) pathway, resulting in the highest ethanol production under these conditions. The amino acid profile clearly suggests that the TCA cycle was primarily active under aerobic cultivation. On the other hand, the presence of high levels of gamma-amino-n-butyric acid (GABA) under anaerobic conditions suggests a functional GABA bypass and a possible block in the TCA cycle at these conditions.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jbiotecen_US
dc.relation.ispartofJournal of biotechnologyen_US
dc.subject.meshAerobiosisen_US
dc.subject.meshAnaerobiosisen_US
dc.subject.meshBioreactors - Microbiologyen_US
dc.subject.meshCitric Acid Cycleen_US
dc.subject.meshEthanol - Metabolismen_US
dc.subject.meshFermentationen_US
dc.subject.meshFusarium - Growth & Development - Metabolismen_US
dc.subject.meshGlucose - Metabolismen_US
dc.subject.meshGlycolysisen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshPentose Phosphate Pathwayen_US
dc.subject.meshTemperatureen_US
dc.subject.meshGamma-Aminobutyric Acid - Analysis - Metabolismen_US
dc.titleIntracellular metabolite profiling of Fusarium oxysporum converting glucose to ethanol.en_US
dc.typeArticleen_US
dc.identifier.emailPanagiotou, G: gipa@hku.hken_US
dc.identifier.authorityPanagiotou, G=rp01725en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jbiotec.2004.09.011-
dc.identifier.pmid15639104en_US
dc.identifier.scopuseid_2-s2.0-14544285139en_US
dc.identifier.volume115en_US
dc.identifier.issue4en_US
dc.identifier.spage425en_US
dc.identifier.epage434en_US
dc.identifier.isiWOS:000226459000010-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridPanagiotou, G=8566179700en_US
dc.identifier.scopusauthoridVillasBôas, SG=8509310900en_US
dc.identifier.scopusauthoridChristakopoulos, P=7006479823en_US
dc.identifier.scopusauthoridNielsen, J=7404066338en_US
dc.identifier.scopusauthoridOlsson, L=7203077540en_US

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