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Article: Establishment of BCWM.1 cell line for Waldenström's macroglobulinemia with productive in vivo engraftment in SCID-hu mice

TitleEstablishment of BCWM.1 cell line for Waldenström's macroglobulinemia with productive in vivo engraftment in SCID-hu mice
Authors
Issue Date2007
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/exphem
Citation
Experimental Hematology, 2007, v. 35 n. 9, p. 1366-1375 How to Cite?
AbstractA significant impairment in understanding the biology and advancing therapeutics for Waldenstrom's macroglobulinemia (WM) has been the lack of a representative cell line and animal model. We, therefore, report on the establishment of the BCWM.1 cell line, which was derived from the long-term culture of CD19 + selected bone marrow lymphoplasmacytic cells isolated from an untreated patient with WM. BCWM.1 cells morphologically resemble lymphoplasmacytic cells (LPC) and propagate in RPMI-1640 medium supplemented with 10% fetal bovine serum. Phenotypic characterization by flow cytometric analysis demonstrated typical WM LPC characteristics: CD5 -, CD10 -, CD19 +, CD20 +, CD23 +, CD27 -, CD38 +, CD138 +, CD40 +, CD52 +, CD70 +, CD117 +, cIgM +, cIgG -, cIgA -, cκ -, cλ +, as well as the survival proteins APRIL and BLYS, and their receptors TACI, BCMA and BAFF-R. Enzyme-linked immunosorbent assay studies demonstrated secretion of IgMλ and soluble CD27. Karyotypic and multicolor fluorescence in situ hybridization studies did not demonstrate cytogenetic abnormalities. Molecular analysis of BCWM.1 cells confirmed clonality by determination of IgH rearrangements. Inoculation of BCWM.1 cells in human bone marrow chips implanted in severe combined immunodeficient-hu mice led to rapid engraftment of tumor cells and serum detection of human IgM, λ, and soluble CD27. These studies support the use of BCWM.1 cells as an appropriate model for the study of WM, which in conjunction with the severe combined immunodeficient-hu mouse model may be used as a convenient model for studies focused on both WM pathogenesis and development of targeted therapies for WM. © 2007 ISEH - Society for Hematology and Stem Cells.
Persistent Identifierhttp://hdl.handle.net/10722/180726
ISSN
2015 Impact Factor: 2.303
2015 SCImago Journal Rankings: 1.341
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDitzel Santos, Den_US
dc.contributor.authorHo, AWen_US
dc.contributor.authorTournilhac, Oen_US
dc.contributor.authorHatjiharissi, Een_US
dc.contributor.authorLeleu, Xen_US
dc.contributor.authorXu, Len_US
dc.contributor.authorTassone, Pen_US
dc.contributor.authorNeri, Pen_US
dc.contributor.authorHunter, ZRen_US
dc.contributor.authorChemaly, MAZen_US
dc.contributor.authorBranagan, ARen_US
dc.contributor.authorManning, RJen_US
dc.contributor.authorPatterson, CJen_US
dc.contributor.authorMoreau, ASen_US
dc.contributor.authorCiccarelli, Ben_US
dc.contributor.authorAdamia, Sen_US
dc.contributor.authorKriangkum, Jen_US
dc.contributor.authorKutok, JLen_US
dc.contributor.authorTai, YTen_US
dc.contributor.authorZhang, Jen_US
dc.contributor.authorPilarski, LMen_US
dc.contributor.authorAnderson, KCen_US
dc.contributor.authorMunshi, Nen_US
dc.contributor.authorTreon, SPen_US
dc.date.accessioned2013-01-28T01:42:01Z-
dc.date.available2013-01-28T01:42:01Z-
dc.date.issued2007en_US
dc.identifier.citationExperimental Hematology, 2007, v. 35 n. 9, p. 1366-1375en_US
dc.identifier.issn0301-472Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/180726-
dc.description.abstractA significant impairment in understanding the biology and advancing therapeutics for Waldenstrom's macroglobulinemia (WM) has been the lack of a representative cell line and animal model. We, therefore, report on the establishment of the BCWM.1 cell line, which was derived from the long-term culture of CD19 + selected bone marrow lymphoplasmacytic cells isolated from an untreated patient with WM. BCWM.1 cells morphologically resemble lymphoplasmacytic cells (LPC) and propagate in RPMI-1640 medium supplemented with 10% fetal bovine serum. Phenotypic characterization by flow cytometric analysis demonstrated typical WM LPC characteristics: CD5 -, CD10 -, CD19 +, CD20 +, CD23 +, CD27 -, CD38 +, CD138 +, CD40 +, CD52 +, CD70 +, CD117 +, cIgM +, cIgG -, cIgA -, cκ -, cλ +, as well as the survival proteins APRIL and BLYS, and their receptors TACI, BCMA and BAFF-R. Enzyme-linked immunosorbent assay studies demonstrated secretion of IgMλ and soluble CD27. Karyotypic and multicolor fluorescence in situ hybridization studies did not demonstrate cytogenetic abnormalities. Molecular analysis of BCWM.1 cells confirmed clonality by determination of IgH rearrangements. Inoculation of BCWM.1 cells in human bone marrow chips implanted in severe combined immunodeficient-hu mice led to rapid engraftment of tumor cells and serum detection of human IgM, λ, and soluble CD27. These studies support the use of BCWM.1 cells as an appropriate model for the study of WM, which in conjunction with the severe combined immunodeficient-hu mouse model may be used as a convenient model for studies focused on both WM pathogenesis and development of targeted therapies for WM. © 2007 ISEH - Society for Hematology and Stem Cells.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/exphemen_US
dc.relation.ispartofExperimental Hematologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshDisease Models, Animalen_US
dc.subject.meshGraft Survivalen_US
dc.subject.meshHumansen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Sciden_US
dc.subject.meshMice, Transgenicen_US
dc.subject.meshTransplantation, Heterologousen_US
dc.subject.meshWaldenstrom Macroglobulinemia - Pathologyen_US
dc.titleEstablishment of BCWM.1 cell line for Waldenström's macroglobulinemia with productive in vivo engraftment in SCID-hu miceen_US
dc.typeArticleen_US
dc.identifier.emailZhang, J: jzhang1@hku.hken_US
dc.identifier.authorityZhang, J=rp01713en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.exphem.2007.05.022en_US
dc.identifier.pmid17761288-
dc.identifier.scopuseid_2-s2.0-34548137710en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34548137710&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume35en_US
dc.identifier.issue9en_US
dc.identifier.spage1366en_US
dc.identifier.epage1375en_US
dc.identifier.isiWOS:000249475500006-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridDitzel Santos, D=12766005600en_US
dc.identifier.scopusauthoridHo, AW=14037306200en_US
dc.identifier.scopusauthoridTournilhac, O=6701700054en_US
dc.identifier.scopusauthoridHatjiharissi, E=6505973525en_US
dc.identifier.scopusauthoridLeleu, X=6701748692en_US
dc.identifier.scopusauthoridXu, L=7404745085en_US
dc.identifier.scopusauthoridTassone, P=7004159835en_US
dc.identifier.scopusauthoridNeri, P=7102228166en_US
dc.identifier.scopusauthoridHunter, ZR=6506892579en_US
dc.identifier.scopusauthoridChemaly, MAZ=25951278300en_US
dc.identifier.scopusauthoridBranagan, AR=6507891568en_US
dc.identifier.scopusauthoridManning, RJ=35805666200en_US
dc.identifier.scopusauthoridPatterson, CJ=34975762100en_US
dc.identifier.scopusauthoridMoreau, AS=8691824400en_US
dc.identifier.scopusauthoridCiccarelli, B=19639837900en_US
dc.identifier.scopusauthoridAdamia, S=8983559600en_US
dc.identifier.scopusauthoridKriangkum, J=6603247296en_US
dc.identifier.scopusauthoridKutok, JL=7003803316en_US
dc.identifier.scopusauthoridTai, YT=7201915839en_US
dc.identifier.scopusauthoridZhang, J=22137260600en_US
dc.identifier.scopusauthoridPilarski, LM=7006873497en_US
dc.identifier.scopusauthoridAnderson, KC=35376064600en_US
dc.identifier.scopusauthoridMunshi, N=7005171559en_US
dc.identifier.scopusauthoridTreon, SP=35434335200en_US

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