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Article: The MicroRNA miR-124 Promotes Neuronal Differentiation by Triggering Brain-Specific Alternative Pre-mRNA Splicing

TitleThe MicroRNA miR-124 Promotes Neuronal Differentiation by Triggering Brain-Specific Alternative Pre-mRNA Splicing
Authors
Issue Date2007
PublisherCell Press. The Journal's web site is located at http://www.elsevier.com/locate/molcel
Citation
Molecular Cell, 2007, v. 27 n. 3, p. 435-448 How to Cite?
AbstractBoth microRNAs and alternative pre-mRNA splicing have been implicated in the development of the nervous system (NS), but functional interactions between these two pathways are poorly understood. We demonstrate that the neuron-specific microRNA miR-124 directly targets PTBP1 (PTB/hnRNP I) mRNA, which encodes a global repressor of alternative pre-mRNA splicing in nonneuronal cells. Among the targets of PTBP1 is a critical cassette exon in the pre-mRNA of PTBP2 (nPTB/brPTB/PTBLP), an NS-enriched PTBP1 homolog. When this exon is skipped, PTBP2 mRNA is subject to nonsense-mediated decay (NMD). During neuronal differentiation, miR-124 reduces PTBP1 levels, leading to the accumulation of correctly spliced PTBP2 mRNA and a dramatic increase in PTBP2 protein. These events culminate in the transition from non-NS to NS-specific alternative splicing patterns. We also present evidence that miR-124 plays a key role in the differentiation of progenitor cells to mature neurons. Thus, miR-124 promotes NS development, at least in part by regulating an intricate network of NS-specific alternative splicing. © 2007 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/180725
ISSN
2015 Impact Factor: 13.958
2015 SCImago Journal Rankings: 13.658
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMakeyev, EVen_US
dc.contributor.authorZhang, Jen_US
dc.contributor.authorCarrasco, MAen_US
dc.contributor.authorManiatis, Ten_US
dc.date.accessioned2013-01-28T01:41:59Z-
dc.date.available2013-01-28T01:41:59Z-
dc.date.issued2007en_US
dc.identifier.citationMolecular Cell, 2007, v. 27 n. 3, p. 435-448en_US
dc.identifier.issn1097-2765en_US
dc.identifier.urihttp://hdl.handle.net/10722/180725-
dc.description.abstractBoth microRNAs and alternative pre-mRNA splicing have been implicated in the development of the nervous system (NS), but functional interactions between these two pathways are poorly understood. We demonstrate that the neuron-specific microRNA miR-124 directly targets PTBP1 (PTB/hnRNP I) mRNA, which encodes a global repressor of alternative pre-mRNA splicing in nonneuronal cells. Among the targets of PTBP1 is a critical cassette exon in the pre-mRNA of PTBP2 (nPTB/brPTB/PTBLP), an NS-enriched PTBP1 homolog. When this exon is skipped, PTBP2 mRNA is subject to nonsense-mediated decay (NMD). During neuronal differentiation, miR-124 reduces PTBP1 levels, leading to the accumulation of correctly spliced PTBP2 mRNA and a dramatic increase in PTBP2 protein. These events culminate in the transition from non-NS to NS-specific alternative splicing patterns. We also present evidence that miR-124 plays a key role in the differentiation of progenitor cells to mature neurons. Thus, miR-124 promotes NS development, at least in part by regulating an intricate network of NS-specific alternative splicing. © 2007 Elsevier Inc. All rights reserved.en_US
dc.languageengen_US
dc.publisherCell Press. The Journal's web site is located at http://www.elsevier.com/locate/molcelen_US
dc.relation.ispartofMolecular Cellen_US
dc.subject.meshAlternative Splicing - Geneticsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBrain - Physiologyen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshExonsen_US
dc.subject.meshGene Expression Regulation, Developmentalen_US
dc.subject.meshHeterogeneous-Nuclear Ribonucleoproteins - Genetics - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshMiceen_US
dc.subject.meshMicrornas - Genetics - Metabolismen_US
dc.subject.meshMitosis - Physiologyen_US
dc.subject.meshNerve Tissue Proteins - Genetics - Metabolismen_US
dc.subject.meshNeuroblastoma - Genetics - Metabolism - Pathologyen_US
dc.subject.meshNeurons - Cytology - Metabolismen_US
dc.subject.meshPolypyrimidine Tract-Binding Protein - Genetics - Metabolismen_US
dc.subject.meshRna Precursors - Genetics - Metabolismen_US
dc.subject.meshRna Processing, Post-Transcriptionalen_US
dc.subject.meshRna, Messenger - Chemistry - Metabolismen_US
dc.subject.meshTransfectionen_US
dc.titleThe MicroRNA miR-124 Promotes Neuronal Differentiation by Triggering Brain-Specific Alternative Pre-mRNA Splicingen_US
dc.typeArticleen_US
dc.identifier.emailZhang, J: jzhang1@hku.hken_US
dc.identifier.authorityZhang, J=rp01713en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.molcel.2007.07.015en_US
dc.identifier.pmid17679093-
dc.identifier.scopuseid_2-s2.0-34547212309en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34547212309&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume27en_US
dc.identifier.issue3en_US
dc.identifier.spage435en_US
dc.identifier.epage448en_US
dc.identifier.isiWOS:000248823400008-
dc.publisher.placeUnited Statesen_US
dc.identifier.f10001089251-
dc.identifier.scopusauthoridMakeyev, EV=6602198520en_US
dc.identifier.scopusauthoridZhang, J=22137260600en_US
dc.identifier.scopusauthoridCarrasco, MA=8741074400en_US
dc.identifier.scopusauthoridManiatis, T=7102213099en_US

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