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- Publisher Website: 10.1016/j.molcel.2007.07.015
- Scopus: eid_2-s2.0-34547212309
- PMID: 17679093
- WOS: WOS:000248823400008
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Article: The MicroRNA miR-124 Promotes Neuronal Differentiation by Triggering Brain-Specific Alternative Pre-mRNA Splicing
Title | The MicroRNA miR-124 Promotes Neuronal Differentiation by Triggering Brain-Specific Alternative Pre-mRNA Splicing |
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Authors | |
Keywords | MOLNEURO RNA |
Issue Date | 2007 |
Publisher | Cell Press. The Journal's web site is located at http://www.elsevier.com/locate/molcel |
Citation | Molecular Cell, 2007, v. 27 n. 3, p. 435-448 How to Cite? |
Abstract | Both microRNAs and alternative pre-mRNA splicing have been implicated in the development of the nervous system (NS), but functional interactions between these two pathways are poorly understood. We demonstrate that the neuron-specific microRNA miR-124 directly targets PTBP1 (PTB/hnRNP I) mRNA, which encodes a global repressor of alternative pre-mRNA splicing in nonneuronal cells. Among the targets of PTBP1 is a critical cassette exon in the pre-mRNA of PTBP2 (nPTB/brPTB/PTBLP), an NS-enriched PTBP1 homolog. When this exon is skipped, PTBP2 mRNA is subject to nonsense-mediated decay (NMD). During neuronal differentiation, miR-124 reduces PTBP1 levels, leading to the accumulation of correctly spliced PTBP2 mRNA and a dramatic increase in PTBP2 protein. These events culminate in the transition from non-NS to NS-specific alternative splicing patterns. We also present evidence that miR-124 plays a key role in the differentiation of progenitor cells to mature neurons. Thus, miR-124 promotes NS development, at least in part by regulating an intricate network of NS-specific alternative splicing. © 2007 Elsevier Inc. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/180725 |
ISSN | 2023 Impact Factor: 14.5 2023 SCImago Journal Rankings: 9.332 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Makeyev, EV | en_US |
dc.contributor.author | Zhang, J | en_US |
dc.contributor.author | Carrasco, MA | en_US |
dc.contributor.author | Maniatis, T | en_US |
dc.date.accessioned | 2013-01-28T01:41:59Z | - |
dc.date.available | 2013-01-28T01:41:59Z | - |
dc.date.issued | 2007 | en_US |
dc.identifier.citation | Molecular Cell, 2007, v. 27 n. 3, p. 435-448 | en_US |
dc.identifier.issn | 1097-2765 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/180725 | - |
dc.description.abstract | Both microRNAs and alternative pre-mRNA splicing have been implicated in the development of the nervous system (NS), but functional interactions between these two pathways are poorly understood. We demonstrate that the neuron-specific microRNA miR-124 directly targets PTBP1 (PTB/hnRNP I) mRNA, which encodes a global repressor of alternative pre-mRNA splicing in nonneuronal cells. Among the targets of PTBP1 is a critical cassette exon in the pre-mRNA of PTBP2 (nPTB/brPTB/PTBLP), an NS-enriched PTBP1 homolog. When this exon is skipped, PTBP2 mRNA is subject to nonsense-mediated decay (NMD). During neuronal differentiation, miR-124 reduces PTBP1 levels, leading to the accumulation of correctly spliced PTBP2 mRNA and a dramatic increase in PTBP2 protein. These events culminate in the transition from non-NS to NS-specific alternative splicing patterns. We also present evidence that miR-124 plays a key role in the differentiation of progenitor cells to mature neurons. Thus, miR-124 promotes NS development, at least in part by regulating an intricate network of NS-specific alternative splicing. © 2007 Elsevier Inc. All rights reserved. | en_US |
dc.language | eng | en_US |
dc.publisher | Cell Press. The Journal's web site is located at http://www.elsevier.com/locate/molcel | en_US |
dc.relation.ispartof | Molecular Cell | en_US |
dc.subject | MOLNEURO | - |
dc.subject | RNA | - |
dc.subject.mesh | Alternative Splicing - Genetics | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Brain - Physiology | en_US |
dc.subject.mesh | Cell Differentiation | en_US |
dc.subject.mesh | Cells, Cultured | en_US |
dc.subject.mesh | Exons | en_US |
dc.subject.mesh | Gene Expression Regulation, Developmental | en_US |
dc.subject.mesh | Heterogeneous-Nuclear Ribonucleoproteins - Genetics - Metabolism | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Micrornas - Genetics - Metabolism | en_US |
dc.subject.mesh | Mitosis - Physiology | en_US |
dc.subject.mesh | Nerve Tissue Proteins - Genetics - Metabolism | en_US |
dc.subject.mesh | Neuroblastoma - Genetics - Metabolism - Pathology | en_US |
dc.subject.mesh | Neurons - Cytology - Metabolism | en_US |
dc.subject.mesh | Polypyrimidine Tract-Binding Protein - Genetics - Metabolism | en_US |
dc.subject.mesh | Rna Precursors - Genetics - Metabolism | en_US |
dc.subject.mesh | Rna Processing, Post-Transcriptional | en_US |
dc.subject.mesh | Rna, Messenger - Chemistry - Metabolism | en_US |
dc.subject.mesh | Transfection | en_US |
dc.title | The MicroRNA miR-124 Promotes Neuronal Differentiation by Triggering Brain-Specific Alternative Pre-mRNA Splicing | en_US |
dc.type | Article | en_US |
dc.identifier.email | Zhang, J: jzhang1@hku.hk | en_US |
dc.identifier.authority | Zhang, J=rp01713 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.molcel.2007.07.015 | en_US |
dc.identifier.pmid | 17679093 | - |
dc.identifier.scopus | eid_2-s2.0-34547212309 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-34547212309&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 27 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 435 | en_US |
dc.identifier.epage | 448 | en_US |
dc.identifier.isi | WOS:000248823400008 | - |
dc.publisher.place | United States | en_US |
dc.identifier.f1000 | 1089251 | - |
dc.identifier.scopusauthorid | Makeyev, EV=6602198520 | en_US |
dc.identifier.scopusauthorid | Zhang, J=22137260600 | en_US |
dc.identifier.scopusauthorid | Carrasco, MA=8741074400 | en_US |
dc.identifier.scopusauthorid | Maniatis, T=7102213099 | en_US |
dc.identifier.issnl | 1097-2765 | - |