File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/S0029-7844(03)00222-9
- Scopus: eid_2-s2.0-0038066292
- PMID: 12738140
- WOS: WOS:000182531500006
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Can amnio-polymerase chain reaction alone replace conventional cytogenetic study for women with positive biochemical screening for fetal Down syndrome?
Title | Can amnio-polymerase chain reaction alone replace conventional cytogenetic study for women with positive biochemical screening for fetal Down syndrome? |
---|---|
Authors | |
Issue Date | 2003 |
Publisher | Lippincott Williams & Wilkins. The Journal's web site is located at http://www.greenjournal.org |
Citation | Obstetrics And Gynecology, 2003, v. 101 n. 5, p. 856-861 How to Cite? |
Abstract | OBJECTIVE: To determine whether amnio-polymerase chain reaction (amnio-PCR) can replace conventional cytogenetic study for confirming the karyotype of fetuses in women with positive biochemical screening for fetal Down syndrome. METHODS: To check the accuracy of this technique in our laboratory, we first compared the amnio-PCR results with those of conventional cytogenetic study in 235 patients referred from June 1999 to December 2001 for prenatal diagnosis in a referral center in Hong Kong. We then reviewed the results of 1526 amniotic fluid cultures performed for positive fetal Down syndrome screening between January 1997 and December 2001 and classified them as detectable or not detectable by amnio-PCR, using the assumption that we had replaced conventional cytogenetic study with amnio-PCR. RESULTS: The 235 amnio-PCR results were all informative, without a false-positive or false-negative result. Of the 1526 cases with positive fetal Down syndrome screening and no ultrasound abnormalities, only two cases of sex chromosome abnormalities and two cases of marker chromosomes would have been missed if conventional cytogenetic study had been replaced by amnio-PCR. CONCLUSION: Amnio-PCR can be an alternative to conventional cytogenetic study for women with positive biochemical screening for fetal Down syndrome and no demonstrable fetal structural abnormality. © 2003 by The American College of Obstetricians and Gynecologists. |
Persistent Identifier | http://hdl.handle.net/10722/180669 |
ISSN | 2023 Impact Factor: 5.7 2023 SCImago Journal Rankings: 2.032 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Leung, WC | en_US |
dc.contributor.author | Lau, ET | en_US |
dc.contributor.author | Lao, TT | en_US |
dc.contributor.author | Tang, MHY | en_US |
dc.date.accessioned | 2013-01-28T01:40:59Z | - |
dc.date.available | 2013-01-28T01:40:59Z | - |
dc.date.issued | 2003 | en_US |
dc.identifier.citation | Obstetrics And Gynecology, 2003, v. 101 n. 5, p. 856-861 | en_US |
dc.identifier.issn | 0029-7844 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/180669 | - |
dc.description.abstract | OBJECTIVE: To determine whether amnio-polymerase chain reaction (amnio-PCR) can replace conventional cytogenetic study for confirming the karyotype of fetuses in women with positive biochemical screening for fetal Down syndrome. METHODS: To check the accuracy of this technique in our laboratory, we first compared the amnio-PCR results with those of conventional cytogenetic study in 235 patients referred from June 1999 to December 2001 for prenatal diagnosis in a referral center in Hong Kong. We then reviewed the results of 1526 amniotic fluid cultures performed for positive fetal Down syndrome screening between January 1997 and December 2001 and classified them as detectable or not detectable by amnio-PCR, using the assumption that we had replaced conventional cytogenetic study with amnio-PCR. RESULTS: The 235 amnio-PCR results were all informative, without a false-positive or false-negative result. Of the 1526 cases with positive fetal Down syndrome screening and no ultrasound abnormalities, only two cases of sex chromosome abnormalities and two cases of marker chromosomes would have been missed if conventional cytogenetic study had been replaced by amnio-PCR. CONCLUSION: Amnio-PCR can be an alternative to conventional cytogenetic study for women with positive biochemical screening for fetal Down syndrome and no demonstrable fetal structural abnormality. © 2003 by The American College of Obstetricians and Gynecologists. | en_US |
dc.language | eng | en_US |
dc.publisher | Lippincott Williams & Wilkins. The Journal's web site is located at http://www.greenjournal.org | en_US |
dc.relation.ispartof | Obstetrics and Gynecology | en_US |
dc.subject.mesh | Adult | en_US |
dc.subject.mesh | Amniocentesis | en_US |
dc.subject.mesh | Down Syndrome - Diagnosis | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Karyotyping | en_US |
dc.subject.mesh | Polymerase Chain Reaction | en_US |
dc.subject.mesh | Pregnancy | en_US |
dc.subject.mesh | Prenatal Diagnosis | en_US |
dc.subject.mesh | Retrospective Studies | en_US |
dc.subject.mesh | Ultrasonography, Prenatal | en_US |
dc.title | Can amnio-polymerase chain reaction alone replace conventional cytogenetic study for women with positive biochemical screening for fetal Down syndrome? | en_US |
dc.type | Article | en_US |
dc.identifier.email | Tang, MHY: mhytang@hkucc.hku.hk | en_US |
dc.identifier.authority | Tang, MHY=rp01701 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/S0029-7844(03)00222-9 | en_US |
dc.identifier.pmid | 12738140 | - |
dc.identifier.scopus | eid_2-s2.0-0038066292 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0038066292&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 101 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.spage | 856 | en_US |
dc.identifier.epage | 861 | en_US |
dc.identifier.isi | WOS:000182531500006 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Leung, WC=7201504435 | en_US |
dc.identifier.scopusauthorid | Lau, ET=7103086081 | en_US |
dc.identifier.scopusauthorid | Lao, TT=7005722132 | en_US |
dc.identifier.scopusauthorid | Tang, MHY=8943401300 | en_US |
dc.identifier.issnl | 0029-7844 | - |