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Conference Paper: Early dynamic changes of mitochondrial morphology in hippocampal neurons in response to β-amyloid

TitleEarly dynamic changes of mitochondrial morphology in hippocampal neurons in response to β-amyloid
Authors
KeywordsOxidative Stress
Mitochondria
Beta Amyloid
Issue Date2012
PublisherAmerican Society of Neuroscience.
Citation
The 42nd Annual Meeting of the Society for Neuroscience (SfN), New Orleans, USA, 13-17 October 2012, p. abstract no. 220.13 How to Cite?
AbstractNeurons are particularly vulnerable to mitochondrial dysfunction due to their high demand of energy. Abnormal mitochondrial dynamics (fission, fusion and movement) has been implicated in the pathogenesis of neurodegenerative diseases including Alzheimer’s disease (AD). Fragmentation of tubular mitochondrial network into spherical organelles during apoptosis has often been linked with mitochondrial dysfunction. However, the relationship between mitochondrial morphology and functions remains obscure; and it is unclear if mitochondrial fragmentation is a straightforward process in disease progression. We hypothesize that early morphological changes of mitochondria do not represent dysfunction of mitochondria. Our aim is to elucidate what are the morphological changes of mitochondria and the inducing factors for such changes. Here, we report that oligomeric beta amyloid (Aβ) induces dynamic changes of mitochondrial morphology at different time points. Aβ triggers morphological changes in mitochondria at early time points (1-6 h), resulting in granular-shaped mitochondria. Despite an obvious change in shape, there are no significant changes in mitochondrial membrane potential and ADP/ATP ratio in granular mitochondria. These granular-shaped mitochondria are morphologically and functionally distinct from fragmented spherical mitochondria during apoptosis. Both treatment of oxidative stress inducing agents and laser-induced mitochondrial-specific oxidative stress by KillerRed-Mito induce granular mitochondria in neurons. These data demonstrate that granular mitochondria might be a result of early oxidative stress in mitochondria. From 6 h onwards, mitochondria undergo dynamic changes in their morphologies according to different cell conditions, and eventually become circular in shape. Our study provides new perspective in mitochondrial morphology and functions at different stages of disease pathogenesis.
DescriptionNanosymposium
Session: 220.Alzheimer's Disease and Other Dementias: Tau and Abeta
Persistent Identifierhttp://hdl.handle.net/10722/180209

 

DC FieldValueLanguage
dc.contributor.authorChang, RCCen_US
dc.contributor.authorHung, HLen_US
dc.contributor.authorWuwongse, Sen_US
dc.contributor.authorZhang, Qen_US
dc.contributor.authorHo, YSen_US
dc.date.accessioned2013-01-21T01:33:39Z-
dc.date.available2013-01-21T01:33:39Z-
dc.date.issued2012en_US
dc.identifier.citationThe 42nd Annual Meeting of the Society for Neuroscience (SfN), New Orleans, USA, 13-17 October 2012, p. abstract no. 220.13en_US
dc.identifier.urihttp://hdl.handle.net/10722/180209-
dc.descriptionNanosymposium-
dc.descriptionSession: 220.Alzheimer's Disease and Other Dementias: Tau and Abeta-
dc.description.abstractNeurons are particularly vulnerable to mitochondrial dysfunction due to their high demand of energy. Abnormal mitochondrial dynamics (fission, fusion and movement) has been implicated in the pathogenesis of neurodegenerative diseases including Alzheimer’s disease (AD). Fragmentation of tubular mitochondrial network into spherical organelles during apoptosis has often been linked with mitochondrial dysfunction. However, the relationship between mitochondrial morphology and functions remains obscure; and it is unclear if mitochondrial fragmentation is a straightforward process in disease progression. We hypothesize that early morphological changes of mitochondria do not represent dysfunction of mitochondria. Our aim is to elucidate what are the morphological changes of mitochondria and the inducing factors for such changes. Here, we report that oligomeric beta amyloid (Aβ) induces dynamic changes of mitochondrial morphology at different time points. Aβ triggers morphological changes in mitochondria at early time points (1-6 h), resulting in granular-shaped mitochondria. Despite an obvious change in shape, there are no significant changes in mitochondrial membrane potential and ADP/ATP ratio in granular mitochondria. These granular-shaped mitochondria are morphologically and functionally distinct from fragmented spherical mitochondria during apoptosis. Both treatment of oxidative stress inducing agents and laser-induced mitochondrial-specific oxidative stress by KillerRed-Mito induce granular mitochondria in neurons. These data demonstrate that granular mitochondria might be a result of early oxidative stress in mitochondria. From 6 h onwards, mitochondria undergo dynamic changes in their morphologies according to different cell conditions, and eventually become circular in shape. Our study provides new perspective in mitochondrial morphology and functions at different stages of disease pathogenesis.-
dc.languageengen_US
dc.publisherAmerican Society of Neuroscience.-
dc.relation.ispartofNeuroscience 2012en_US
dc.subjectOxidative Stress-
dc.subjectMitochondria-
dc.subjectBeta Amyloid-
dc.titleEarly dynamic changes of mitochondrial morphology in hippocampal neurons in response to β-amyloiden_US
dc.typeConference_Paperen_US
dc.identifier.emailChang, RCC: rccchang@hku.hken_US
dc.identifier.emailHo, YS: janiceys@hku.hken_US
dc.identifier.authorityChang, RCC=rp00470en_US
dc.identifier.hkuros212913en_US
dc.identifier.spageabstract no. 220.13en_US
dc.identifier.epageabstract no. 220.13en_US
dc.publisher.placeUnited States-

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