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Article: Influenza virus infections among a sample of hospital attendees in Ragama, Sri Lanka.

TitleInfluenza virus infections among a sample of hospital attendees in Ragama, Sri Lanka.
Authors
Issue Date2010
Citation
The Ceylon Medical Journal, 2010, v. 55 n. 2, p. 40-44 How to Cite?
AbstractOBJECTIVES: This study was carried out to define the types of influenza viruses circulating among humans and to understand the seasonality of influenza virus activity. Such information is essential for deciding on influenza vaccination strategy and on the appropriate time for delivering influenza vaccination, if such a vaccination policy was decided to be a priority. METHOD: During the period July 2003 - August 2004, 300 nasopharyngeal aspirates (NPA) were obtained from a systematic sample of patients reported to Out-patient Department, Colombo North Teaching Hospital, Ragama with < or =4 days history of acute respiratory tract infection (ARTI). The clinical signs and symptoms of the patients were prospectively recorded. Isolation of the influenza virus was carried out by inoculating in Madin Darby Canine Kidney cell line (MDCK). The isolates were identified by immunofluorescence assay and characterised by haemagglutination inhibition test. RT-PCR was carried out on all NPA samples. Genetic sequencing and phylogenetic analysis of the haemagglutinin gene of representative viruses were carried out. RESULTS: Twenty three influenza A and nine influenza B viruses were isolated by cell culture methods. Influenza A H3N2 Panama/2000/99-like viruses were isolated in 8% of patients with ARTI and influenza B/Sichuan/ 379/99-like viruses were isolated in 3%. Twenty eight influenza A virus infections were identified by the RT-PCR method. Phylogenetic analysis was carried out with data from other H3-subtype viruses isolated worldwide. The Sri Lanka viruses are antigenically and genetically similar to those in the northern and southern hemispheres. CONCLUSIONS: Influenza viruses circulate at different times of the year and is the aetiological agent causing 11% of all ARTI. Influenza activity corresponded to a peak in rainfall; however the correlation of influenza virus activity with rainfall is not invariable. The Sri Lankan isolates of 2003-4 were genetically related to the influenza A viruses circulating around the globe.
Persistent Identifierhttp://hdl.handle.net/10722/179828
ISSN
2015 SCImago Journal Rankings: 0.185

 

DC FieldValueLanguage
dc.contributor.authorPerera, KVen_US
dc.contributor.authorChan, KHen_US
dc.contributor.authorMa, Een_US
dc.contributor.authorPeiris, JSen_US
dc.date.accessioned2012-12-19T10:05:15Z-
dc.date.available2012-12-19T10:05:15Z-
dc.date.issued2010en_US
dc.identifier.citationThe Ceylon Medical Journal, 2010, v. 55 n. 2, p. 40-44en_US
dc.identifier.issn0009-0875en_US
dc.identifier.urihttp://hdl.handle.net/10722/179828-
dc.description.abstractOBJECTIVES: This study was carried out to define the types of influenza viruses circulating among humans and to understand the seasonality of influenza virus activity. Such information is essential for deciding on influenza vaccination strategy and on the appropriate time for delivering influenza vaccination, if such a vaccination policy was decided to be a priority. METHOD: During the period July 2003 - August 2004, 300 nasopharyngeal aspirates (NPA) were obtained from a systematic sample of patients reported to Out-patient Department, Colombo North Teaching Hospital, Ragama with < or =4 days history of acute respiratory tract infection (ARTI). The clinical signs and symptoms of the patients were prospectively recorded. Isolation of the influenza virus was carried out by inoculating in Madin Darby Canine Kidney cell line (MDCK). The isolates were identified by immunofluorescence assay and characterised by haemagglutination inhibition test. RT-PCR was carried out on all NPA samples. Genetic sequencing and phylogenetic analysis of the haemagglutinin gene of representative viruses were carried out. RESULTS: Twenty three influenza A and nine influenza B viruses were isolated by cell culture methods. Influenza A H3N2 Panama/2000/99-like viruses were isolated in 8% of patients with ARTI and influenza B/Sichuan/ 379/99-like viruses were isolated in 3%. Twenty eight influenza A virus infections were identified by the RT-PCR method. Phylogenetic analysis was carried out with data from other H3-subtype viruses isolated worldwide. The Sri Lanka viruses are antigenically and genetically similar to those in the northern and southern hemispheres. CONCLUSIONS: Influenza viruses circulate at different times of the year and is the aetiological agent causing 11% of all ARTI. Influenza activity corresponded to a peak in rainfall; however the correlation of influenza virus activity with rainfall is not invariable. The Sri Lankan isolates of 2003-4 were genetically related to the influenza A viruses circulating around the globe.en_US
dc.languageengen_US
dc.relation.ispartofThe Ceylon medical journalen_US
dc.subject.meshHumansen_US
dc.subject.meshInfluenza A Virus - Genetics - Isolation & Purificationen_US
dc.subject.meshInfluenza B Virus - Isolation & Purificationen_US
dc.subject.meshInfluenza, Human - Epidemiology - Geneticsen_US
dc.subject.meshRainen_US
dc.subject.meshSeasonsen_US
dc.subject.meshSeroepidemiologic Studiesen_US
dc.subject.meshSri Lanka - Epidemiologyen_US
dc.titleInfluenza virus infections among a sample of hospital attendees in Ragama, Sri Lanka.en_US
dc.typeArticleen_US
dc.identifier.emailPeiris, JS: malik@hkucc.hku.hken_US
dc.identifier.authorityPeiris, JS=rp00410en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid20645542-
dc.identifier.scopuseid_2-s2.0-77955887329en_US
dc.identifier.volume55en_US
dc.identifier.issue2en_US
dc.identifier.spage40en_US
dc.identifier.epage44en_US
dc.identifier.scopusauthoridPerera, KV=24824987100en_US
dc.identifier.scopusauthoridChan, KH=10539510000en_US
dc.identifier.scopusauthoridMa, E=24725277400en_US
dc.identifier.scopusauthoridPeiris, JS=7005486823en_US

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