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Article: Universal primer set for the full-length amplification of all influenza A viruses

TitleUniversal primer set for the full-length amplification of all influenza A viruses
Authors
Issue Date2001
PublisherSpringer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705
Citation
Archives Of Virology, 2001, v. 146 n. 12, p. 2275-2289 How to Cite?
AbstractTo systematically identify and analyze the 15 HA and 9 NA subtypes of influenza A virus, we need reliable, simple methods that not only characterize partial sequences but analyze the entire influenza A genome. We designed primers based on the fact that the 15 and 21 terminal segment specific nucleotides of the genomic viral RNA are conserved between all influenza A viruses and unique for each segment. The primers designed for each segment contain influenza virus specific nucleotides at their 3′-end and non-influenza virus nucleotides at the 5′-end. With this set of primers, we were able to amplify all eight segments of N1, N2, N4, N5, and N8 subtypes. For N3, N6, N7, and N9 subtypes, the segment specific sequences of the neuraminidase genes are different. Therefore, we optimized the primer design to allow the amplification of those neuraminidase genes as well. The resultant primer set is suitable for all influenza A viruses to generate full-length cDNAs, to subtype viruses, to sequence their DNA, and to construct expression plasmids for reverse genetics systems.
Persistent Identifierhttp://hdl.handle.net/10722/179771
ISSN
2015 Impact Factor: 2.255
2015 SCImago Journal Rankings: 1.086
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHoffmann, Een_US
dc.contributor.authorStech, Jen_US
dc.contributor.authorGuan, Yen_US
dc.contributor.authorWebster, RGen_US
dc.contributor.authorPerez, DRen_US
dc.date.accessioned2012-12-19T10:04:28Z-
dc.date.available2012-12-19T10:04:28Z-
dc.date.issued2001en_US
dc.identifier.citationArchives Of Virology, 2001, v. 146 n. 12, p. 2275-2289en_US
dc.identifier.issn0304-8608en_US
dc.identifier.urihttp://hdl.handle.net/10722/179771-
dc.description.abstractTo systematically identify and analyze the 15 HA and 9 NA subtypes of influenza A virus, we need reliable, simple methods that not only characterize partial sequences but analyze the entire influenza A genome. We designed primers based on the fact that the 15 and 21 terminal segment specific nucleotides of the genomic viral RNA are conserved between all influenza A viruses and unique for each segment. The primers designed for each segment contain influenza virus specific nucleotides at their 3′-end and non-influenza virus nucleotides at the 5′-end. With this set of primers, we were able to amplify all eight segments of N1, N2, N4, N5, and N8 subtypes. For N3, N6, N7, and N9 subtypes, the segment specific sequences of the neuraminidase genes are different. Therefore, we optimized the primer design to allow the amplification of those neuraminidase genes as well. The resultant primer set is suitable for all influenza A viruses to generate full-length cDNAs, to subtype viruses, to sequence their DNA, and to construct expression plasmids for reverse genetics systems.en_US
dc.languageengen_US
dc.publisherSpringer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705en_US
dc.relation.ispartofArchives of Virologyen_US
dc.subject.meshChilden_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshDna Primersen_US
dc.subject.meshGene Amplificationen_US
dc.subject.meshHemagglutinin Glycoproteins, Influenza Virus - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshInfluenza A Virus - Classification - Geneticsen_US
dc.subject.meshInfluenza, Human - Virologyen_US
dc.subject.meshNeuraminidase - Geneticsen_US
dc.subject.meshNucleic Acid Amplification Techniquesen_US
dc.subject.meshOligonucleotidesen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.titleUniversal primer set for the full-length amplification of all influenza A virusesen_US
dc.typeArticleen_US
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_US
dc.identifier.authorityGuan, Y=rp00397en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s007050170002en_US
dc.identifier.pmid11811679-
dc.identifier.scopuseid_2-s2.0-0035559445en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035559445&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume146en_US
dc.identifier.issue12en_US
dc.identifier.spage2275en_US
dc.identifier.epage2289en_US
dc.identifier.isiWOS:000172842700002-
dc.publisher.placeAustriaen_US
dc.identifier.scopusauthoridHoffmann, E=7201369718en_US
dc.identifier.scopusauthoridStech, J=23028967700en_US
dc.identifier.scopusauthoridGuan, Y=7202924055en_US
dc.identifier.scopusauthoridWebster, RG=36048363100en_US
dc.identifier.scopusauthoridPerez, DR=7101996480en_US

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