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Article: Prevalence of tick-borne encephalitis virus in Ixodes ricinus ticks in Finland

TitlePrevalence of tick-borne encephalitis virus in Ixodes ricinus ticks in Finland
Authors
Issue Date2001
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763
Citation
Journal Of Medical Virology, 2001, v. 64 n. 1, p. 21-28 How to Cite?
AbstractApproximately 20 cases of tick-borne encephalitis (TBE) occur annually in Finland. The known endemic areas are situated mainly in the archipelago and coastal regions of Finland, with highest incidence in Åland islands. Ixodes ricinus panels collected in 1996-1997 from two endemic areas were screened for the presence of RNA. Two distinct RT-PCR methods were applied, and were shown to have an approximate detection limit of 10 focus forming doses (FFD)/100 μl. One out of 20 pools (a total of 139 ticks) from Helsinki Isosaari Island and one out of 48 pools (a total of 450 ticks) from Åland were positive with both methods, whereas the remaining pools were negative. The observed overall frequency (0.34%) in ticks in endemic areas of Finland, was similar to the low incidence found by virus isolation in mice in the 1960s (0.5%). Viral RNA was detectable in a diluted sample representing 0.005% of a positive pool often nymphs suggesting that the viral RNA load within an infected tick pool was approximately equivalent to 20,000-200,000 FFD. Sequence analysis did not show geographical clustering of the Finnish strains, suggesting an independent emergence of different TBE foci from the south. TBE virus RNA positive ticks were not found in I. ricinus panels consisting of 130 pools (726 ticks) from Helsinki city parks or 41 pools (197 ticks) from Võrmsi Island in Estonia. © 2001 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/179770
ISSN
2015 Impact Factor: 1.998
2015 SCImago Journal Rankings: 1.015
References

 

DC FieldValueLanguage
dc.contributor.authorTong, CYWen_US
dc.contributor.authorBakran, Aen_US
dc.contributor.authorWilliams, Hen_US
dc.contributor.authorCuevas, LEen_US
dc.contributor.authorPeiris, JSMen_US
dc.contributor.authorHart, CAen_US
dc.date.accessioned2012-12-19T10:04:27Z-
dc.date.available2012-12-19T10:04:27Z-
dc.date.issued2001en_US
dc.identifier.citationJournal Of Medical Virology, 2001, v. 64 n. 1, p. 21-28en_US
dc.identifier.issn0146-6615en_US
dc.identifier.urihttp://hdl.handle.net/10722/179770-
dc.description.abstractApproximately 20 cases of tick-borne encephalitis (TBE) occur annually in Finland. The known endemic areas are situated mainly in the archipelago and coastal regions of Finland, with highest incidence in Åland islands. Ixodes ricinus panels collected in 1996-1997 from two endemic areas were screened for the presence of RNA. Two distinct RT-PCR methods were applied, and were shown to have an approximate detection limit of 10 focus forming doses (FFD)/100 μl. One out of 20 pools (a total of 139 ticks) from Helsinki Isosaari Island and one out of 48 pools (a total of 450 ticks) from Åland were positive with both methods, whereas the remaining pools were negative. The observed overall frequency (0.34%) in ticks in endemic areas of Finland, was similar to the low incidence found by virus isolation in mice in the 1960s (0.5%). Viral RNA was detectable in a diluted sample representing 0.005% of a positive pool often nymphs suggesting that the viral RNA load within an infected tick pool was approximately equivalent to 20,000-200,000 FFD. Sequence analysis did not show geographical clustering of the Finnish strains, suggesting an independent emergence of different TBE foci from the south. TBE virus RNA positive ticks were not found in I. ricinus panels consisting of 130 pools (726 ticks) from Helsinki city parks or 41 pools (197 ticks) from Võrmsi Island in Estonia. © 2001 Wiley-Liss, Inc.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763en_US
dc.relation.ispartofJournal of Medical Virologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshEncephalitis Viruses, Tick-Borne - Genetics - Isolation & Purificationen_US
dc.subject.meshEncephalitis, Tick-Borne - Epidemiologyen_US
dc.subject.meshEndemic Diseasesen_US
dc.subject.meshFinland - Epidemiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshIxodes - Virologyen_US
dc.subject.meshMiceen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPrevalenceen_US
dc.subject.meshRna, Viral - Analysisen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshSequence Alignmenten_US
dc.subject.meshSequence Analysis - Methodsen_US
dc.subject.meshViral Loaden_US
dc.subject.meshViral Nonstructural Proteins - Geneticsen_US
dc.subject.meshVirus Replicationen_US
dc.titlePrevalence of tick-borne encephalitis virus in Ixodes ricinus ticks in Finlanden_US
dc.typeArticleen_US
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_US
dc.identifier.authorityPeiris, JSM=rp00410en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/jmv.1012en_US
dc.identifier.pmid11285564-
dc.identifier.scopuseid_2-s2.0-0035083527en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035083527&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume64en_US
dc.identifier.issue1en_US
dc.identifier.spage21en_US
dc.identifier.epage28en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridTong, CYW=37762182100en_US
dc.identifier.scopusauthoridBakran, A=7004639540en_US
dc.identifier.scopusauthoridWilliams, H=7402822319en_US
dc.identifier.scopusauthoridCuevas, LE=7005663531en_US
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_US
dc.identifier.scopusauthoridHart, CA=7402569123en_US

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