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Article: Mutagenic analysis of the 5' arm of the influenza A virus virion RNA promoter defines the sequence requirements for endonuclease activity

TitleMutagenic analysis of the 5' arm of the influenza A virus virion RNA promoter defines the sequence requirements for endonuclease activity
Authors
Issue Date2001
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
Citation
Journal Of Virology, 2001, v. 75 n. 1, p. 134-142 How to Cite?
AbstractShort synthetic influenza virus-like RNAs derived from influenza virus promoter sequences were examined for their ability to stimulate the endonuclease activity of recombinant influenza virus polymerase complexes in vitro, an activity that is required for the cap-snatching activity of primers from host pre-mRNA. An extensive set of point mutants of the 5' arm of the influenza A virus viral RNA (vRNA) was constructed to determine the cis-acting elements which influenced endonuclease activity. Activity was found to be dependent on three features of the conserved vRNA termini: (i) the presence of the 5' hairpin loop structure, (ii) the identity of residues at positions 5 and 10 bases from the 5' terminus, and (iii) the presence of base pair interactions between the 5' and 3' segment ends. Further experiments discounted a role for the vRNA U track in endonuclease activation. This study represents the first mutagenic analysis of the influenza virus promoter with regard to endonuclease activity.
Persistent Identifierhttp://hdl.handle.net/10722/179768
ISSN
2015 Impact Factor: 4.606
2015 SCImago Journal Rankings: 3.347
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeahy, MBen_US
dc.contributor.authorPritlove, DCen_US
dc.contributor.authorPoon, LLMen_US
dc.contributor.authorBrownlee, GGen_US
dc.date.accessioned2012-12-19T10:04:26Z-
dc.date.available2012-12-19T10:04:26Z-
dc.date.issued2001en_US
dc.identifier.citationJournal Of Virology, 2001, v. 75 n. 1, p. 134-142en_US
dc.identifier.issn0022-538Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/179768-
dc.description.abstractShort synthetic influenza virus-like RNAs derived from influenza virus promoter sequences were examined for their ability to stimulate the endonuclease activity of recombinant influenza virus polymerase complexes in vitro, an activity that is required for the cap-snatching activity of primers from host pre-mRNA. An extensive set of point mutants of the 5' arm of the influenza A virus viral RNA (vRNA) was constructed to determine the cis-acting elements which influenced endonuclease activity. Activity was found to be dependent on three features of the conserved vRNA termini: (i) the presence of the 5' hairpin loop structure, (ii) the identity of residues at positions 5 and 10 bases from the 5' terminus, and (iii) the presence of base pair interactions between the 5' and 3' segment ends. Further experiments discounted a role for the vRNA U track in endonuclease activation. This study represents the first mutagenic analysis of the influenza virus promoter with regard to endonuclease activity.en_US
dc.languageengen_US
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/en_US
dc.relation.ispartofJournal of Virologyen_US
dc.subject.meshEndonucleases - Metabolismen_US
dc.subject.meshHela Cellsen_US
dc.subject.meshHumansen_US
dc.subject.meshInfluenza A Virus - Geneticsen_US
dc.subject.meshPoint Mutationen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshRna Replicase - Metabolismen_US
dc.subject.meshRna, Complementary - Chemistryen_US
dc.subject.meshRna, Viral - Chemistryen_US
dc.subject.meshVirion - Geneticsen_US
dc.titleMutagenic analysis of the 5' arm of the influenza A virus virion RNA promoter defines the sequence requirements for endonuclease activityen_US
dc.typeArticleen_US
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_US
dc.identifier.authorityPoon, LLM=rp00484en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1128/JVI.75.1.134-142.2001en_US
dc.identifier.pmid11119582-
dc.identifier.scopuseid_2-s2.0-0034749284en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034749284&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume75en_US
dc.identifier.issue1en_US
dc.identifier.spage134en_US
dc.identifier.epage142en_US
dc.identifier.isiWOS:000165863000016-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLeahy, MB=7101611643en_US
dc.identifier.scopusauthoridPritlove, DC=6602418386en_US
dc.identifier.scopusauthoridPoon, LLM=7005441747en_US
dc.identifier.scopusauthoridBrownlee, GG=35994351900en_US

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