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Article: Messenger RNAs that are not synthesized by RNA polymerase II can be 3′ end cleaved and polyadenylated

TitleMessenger RNAs that are not synthesized by RNA polymerase II can be 3′ end cleaved and polyadenylated
Authors
Issue Date2000
PublisherNature Publishing Group. The Journal's web site is located at http://www.emboreports.org
Citation
Embo Reports, 2000, v. 1 n. 6, p. 513-518 How to Cite?
AbstractThe poly(A) tail of influenza virus mRNAs is synthesized by the viral RNA polymerase by reiterative copying of a U5-7 sequence near the 5′ end of the viral RNA (vRNA) template. We have engineered a vRNA molecule by replacing its viral U6 poly(A) site with a negative-sense eukaryotic polyadenylation signal. The vRNA was transcribed by the viral RNA polymerase and the transcription product was processed by the cellular 3′ end processing machinery in vivo. According to the current model, 3′ end processing of eukaryotic pre-mRNAs is coupled to cellular RNA polymerase II (pol II) transcription; thus only RNAs synthesized by pol II are believed to be polyadenylated efficiently. Our results show that the cellular polyadenylation machinery is nevertheless able to recognize and process RNA transcripts that are not synthesized by pol II, indicating that synthesis by pol II is not an absolute requirement for 3′ end processing in vivo.
Persistent Identifierhttp://hdl.handle.net/10722/179766
ISSN
2015 Impact Factor: 7.739
2015 SCImago Journal Rankings: 4.291
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFodor, Een_US
dc.contributor.authorMikulasova, Aen_US
dc.contributor.authorMingay, LJen_US
dc.contributor.authorPoon, LLMen_US
dc.contributor.authorBrownlee, GGen_US
dc.date.accessioned2012-12-19T10:04:26Z-
dc.date.available2012-12-19T10:04:26Z-
dc.date.issued2000en_US
dc.identifier.citationEmbo Reports, 2000, v. 1 n. 6, p. 513-518en_US
dc.identifier.issn1469-221Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/179766-
dc.description.abstractThe poly(A) tail of influenza virus mRNAs is synthesized by the viral RNA polymerase by reiterative copying of a U5-7 sequence near the 5′ end of the viral RNA (vRNA) template. We have engineered a vRNA molecule by replacing its viral U6 poly(A) site with a negative-sense eukaryotic polyadenylation signal. The vRNA was transcribed by the viral RNA polymerase and the transcription product was processed by the cellular 3′ end processing machinery in vivo. According to the current model, 3′ end processing of eukaryotic pre-mRNAs is coupled to cellular RNA polymerase II (pol II) transcription; thus only RNAs synthesized by pol II are believed to be polyadenylated efficiently. Our results show that the cellular polyadenylation machinery is nevertheless able to recognize and process RNA transcripts that are not synthesized by pol II, indicating that synthesis by pol II is not an absolute requirement for 3′ end processing in vivo.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.emboreports.orgen_US
dc.relation.ispartofEMBO Reportsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshHumansen_US
dc.subject.meshInfluenza, Human - Geneticsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMutagenesisen_US
dc.subject.meshNeuraminidase - Geneticsen_US
dc.subject.meshPlasmids - Metabolismen_US
dc.subject.meshPoly A - Metabolismen_US
dc.subject.meshRna Polymerase Ii - Metabolismen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshRna, Viral - Physiology - Ultrastructureen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSingle-Strand Specific Dna And Rna Endonucleases - Metabolismen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshTransfectionen_US
dc.titleMessenger RNAs that are not synthesized by RNA polymerase II can be 3′ end cleaved and polyadenylateden_US
dc.typeArticleen_US
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_US
dc.identifier.authorityPoon, LLM=rp00484en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/embo-reports/kvd111-
dc.identifier.pmid11263496-
dc.identifier.scopuseid_2-s2.0-0034574614en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034574614&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume1en_US
dc.identifier.issue6en_US
dc.identifier.spage513en_US
dc.identifier.epage518en_US
dc.identifier.isiWOS:000166006200014-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridFodor, E=7006539874en_US
dc.identifier.scopusauthoridMikulasova, A=6505981487en_US
dc.identifier.scopusauthoridMingay, LJ=6507504084en_US
dc.identifier.scopusauthoridPoon, LLM=7005441747en_US
dc.identifier.scopusauthoridBrownlee, GG=35994351900en_US

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