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Article: Polyuridylated mRNA synthesized by a recombinant influenza virus is defective in nuclear export

TitlePolyuridylated mRNA synthesized by a recombinant influenza virus is defective in nuclear export
Authors
Issue Date2000
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
Citation
Journal Of Virology, 2000, v. 74 n. 1, p. 418-427 How to Cite?
AbstractThe poly(A) tail of influenza virus mRNA is synthesized by reiterative copying of a U track near the 5' end of the virion RNA (vRNA) template by the viral RNA polymerase. We have engineered a novel influenza A/WSN/33 virus which contains a neuraminidase (NA) vRNA with its U track mutated into an A track. Instead of synthesizing poly(A)-tailed NA mRNA, this novel virus synthesizes poly(U)-tailed NA mRNA. In infected cells, most poly(U)-tailed NA mRNA was retained in the nucleus, while most control polyadenylated NA mRNA was transported to the cytoplasm. These results suggest that the poly(A) tail is important for efficient nuclear export of NA mRNA. The mutant virus produced a reduced amount of NA and showed an attenuated phenotype, suggesting that poly(A) signal mutants of this type might be useful as potential live attenuated virus vaccines. In addition, this virus mutant might provide a useful model to further elucidate the basic mechanisms of mRNA nuclear export.
Persistent Identifierhttp://hdl.handle.net/10722/179765
ISSN
2015 Impact Factor: 4.606
2015 SCImago Journal Rankings: 3.347
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPoon, LLMen_US
dc.contributor.authorFodor, Een_US
dc.contributor.authorBrownlee, GGen_US
dc.date.accessioned2012-12-19T10:04:25Z-
dc.date.available2012-12-19T10:04:25Z-
dc.date.issued2000en_US
dc.identifier.citationJournal Of Virology, 2000, v. 74 n. 1, p. 418-427en_US
dc.identifier.issn0022-538Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/179765-
dc.description.abstractThe poly(A) tail of influenza virus mRNA is synthesized by reiterative copying of a U track near the 5' end of the virion RNA (vRNA) template by the viral RNA polymerase. We have engineered a novel influenza A/WSN/33 virus which contains a neuraminidase (NA) vRNA with its U track mutated into an A track. Instead of synthesizing poly(A)-tailed NA mRNA, this novel virus synthesizes poly(U)-tailed NA mRNA. In infected cells, most poly(U)-tailed NA mRNA was retained in the nucleus, while most control polyadenylated NA mRNA was transported to the cytoplasm. These results suggest that the poly(A) tail is important for efficient nuclear export of NA mRNA. The mutant virus produced a reduced amount of NA and showed an attenuated phenotype, suggesting that poly(A) signal mutants of this type might be useful as potential live attenuated virus vaccines. In addition, this virus mutant might provide a useful model to further elucidate the basic mechanisms of mRNA nuclear export.en_US
dc.languageengen_US
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/en_US
dc.relation.ispartofJournal of Virologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBiological Transporten_US
dc.subject.meshCell Nucleus - Metabolismen_US
dc.subject.meshChick Embryoen_US
dc.subject.meshDna Primersen_US
dc.subject.meshInfluenza A Virus - Geneticsen_US
dc.subject.meshMutationen_US
dc.subject.meshNeuraminidase - Geneticsen_US
dc.subject.meshNucleic Acid Conformationen_US
dc.subject.meshPoly U - Metabolismen_US
dc.subject.meshRna, Messenger - Biosynthesis - Chemistry - Metabolismen_US
dc.subject.meshRecombination, Geneticen_US
dc.titlePolyuridylated mRNA synthesized by a recombinant influenza virus is defective in nuclear exporten_US
dc.typeArticleen_US
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_US
dc.identifier.authorityPoon, LLM=rp00484en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1128/JVI.74.1.418-427.2000-
dc.identifier.pmid10590131-
dc.identifier.scopuseid_2-s2.0-0033988506en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033988506&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume74en_US
dc.identifier.issue1en_US
dc.identifier.spage418en_US
dc.identifier.epage427en_US
dc.identifier.isiWOS:000084137100048-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridPoon, LLM=7005441747en_US
dc.identifier.scopusauthoridFodor, E=7006539874en_US
dc.identifier.scopusauthoridBrownlee, GG=35994351900en_US

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