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Article: A hairpin loop at the 5' end of influenza A virus virion RNA is required for synthesis of poly(A)+ mRNA in vitro

TitleA hairpin loop at the 5' end of influenza A virus virion RNA is required for synthesis of poly(A)+ mRNA in vitro
Authors
Issue Date1999
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
Citation
Journal Of Virology, 1999, v. 73 n. 3, p. 2109-2114 How to Cite?
AbstractWe present evidence, based on extensive mutagenesis, that a hairpin loop at the 5' end of influenza A virus virion RNA (vRNA) is required for the synthesis of polyadenylated mRNA from model vRNA templates in vitro. The hairpin loop, which we term the vRNA 5' hook, contains a stem of 2 bp formed by the second and third residues pairing with the ninth and eighth residues, respectively, and a 4-nucleotide loop composed of the intervening residues 4 to 7. Disruption of the base pairs of the vRNA 5' hook by introducing point mutations prevented polyadenylation, except in two mutants where a G-U base pair reformed. The polyadenylation activity of point mutants could be rescued by constructing double mutants with reformed base pairs in the stem of the vRNA 5' hook. These results suggest that base pairing rather than a particular nucleotide sequence was critical. We also show that mutation of the analogous region in the 3' arm of vRNA did not interfere with the synthesis of polyadenylated mRNA, suggesting that a hook structure in the 3' arm is not required for transcription of polyadenylated mRNA in vitro.
Persistent Identifierhttp://hdl.handle.net/10722/179762
ISSN
2015 Impact Factor: 4.606
2015 SCImago Journal Rankings: 3.347
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPritlove, DCen_US
dc.contributor.authorPoon, LLMen_US
dc.contributor.authorDevenish, LJen_US
dc.contributor.authorLeahy, MBen_US
dc.contributor.authorBrownlee, GGen_US
dc.date.accessioned2012-12-19T10:04:24Z-
dc.date.available2012-12-19T10:04:24Z-
dc.date.issued1999en_US
dc.identifier.citationJournal Of Virology, 1999, v. 73 n. 3, p. 2109-2114en_US
dc.identifier.issn0022-538Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/179762-
dc.description.abstractWe present evidence, based on extensive mutagenesis, that a hairpin loop at the 5' end of influenza A virus virion RNA (vRNA) is required for the synthesis of polyadenylated mRNA from model vRNA templates in vitro. The hairpin loop, which we term the vRNA 5' hook, contains a stem of 2 bp formed by the second and third residues pairing with the ninth and eighth residues, respectively, and a 4-nucleotide loop composed of the intervening residues 4 to 7. Disruption of the base pairs of the vRNA 5' hook by introducing point mutations prevented polyadenylation, except in two mutants where a G-U base pair reformed. The polyadenylation activity of point mutants could be rescued by constructing double mutants with reformed base pairs in the stem of the vRNA 5' hook. These results suggest that base pairing rather than a particular nucleotide sequence was critical. We also show that mutation of the analogous region in the 3' arm of vRNA did not interfere with the synthesis of polyadenylated mRNA, suggesting that a hook structure in the 3' arm is not required for transcription of polyadenylated mRNA in vitro.en_US
dc.languageengen_US
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/en_US
dc.relation.ispartofJournal of Virologyen_US
dc.subject.meshInfluenza A Virus - Geneticsen_US
dc.subject.meshMutagenesisen_US
dc.subject.meshRna, Messenger - Biosynthesisen_US
dc.subject.meshRna, Viral - Chemistryen_US
dc.subject.meshStructure-Activity Relationshipen_US
dc.subject.meshVirion - Geneticsen_US
dc.titleA hairpin loop at the 5' end of influenza A virus virion RNA is required for synthesis of poly(A)+ mRNA in vitroen_US
dc.typeArticleen_US
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_US
dc.identifier.authorityPoon, LLM=rp00484en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9971793-
dc.identifier.scopuseid_2-s2.0-0032980704en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032980704&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume73en_US
dc.identifier.issue3en_US
dc.identifier.spage2109en_US
dc.identifier.epage2114en_US
dc.identifier.isiWOS:000078603800043-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridPritlove, DC=6602418386en_US
dc.identifier.scopusauthoridPoon, LLM=7005441747en_US
dc.identifier.scopusauthoridDevenish, LJ=6506084407en_US
dc.identifier.scopusauthoridLeahy, MB=7101611643en_US
dc.identifier.scopusauthoridBrownlee, GG=35994351900en_US

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