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Article: Correlation between the detection of viral DNA by the polymerase chain reaction in peripheral blood leukocytes and serological responses to human herpesvirus 6, human herpesvirus 7, and cytomegalovirus in renal allograft recipients

TitleCorrelation between the detection of viral DNA by the polymerase chain reaction in peripheral blood leukocytes and serological responses to human herpesvirus 6, human herpesvirus 7, and cytomegalovirus in renal allograft recipients
Authors
Issue Date1997
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763
Citation
Journal of Medical Virology, 1997, v. 53 n. 3, p. 288-294 How to Cite?
AbstractDiagnosis of significant infections by human herpesvirus 6 (HHV6) AND 7 (HHV7) in transplant patients has proved difficult because both viruses are ubiquitous and can cause persistent infections in their hosts. The significance of viral DNA detected in peripheral blood leukocytes (PBLs; DNAemial) by PCR is therefore unclear. The interpretation of serological results is complicated by the fact that both primary and secondary infections with other herpesviruses may be associated with a concurrent antibody response to HHV6. Fifty-four renal allograft recipients were studied prospectively and their serological response to HHV6, HHV7 and CMV were compared with the detection of viral DNAemia from the homologous heterologous viruses. Serum and heparinised blood samples were collected prospectively from 54 real allograft recipients. DNA was extracted from PBLs and tested for the presence of HHV6, HHV7 and CMV DNA by PCR. Antibodies to HHV6 and HHV7 were measured by an indirect immunofluorescence test and to CMV by an anticomplement immunofluorescence (ACIF) test. CMV IgM antibodies were detected by a commercial enzyme immunoassay. CMV and HHV7 DNAemia were each significantly associated with serological responses to the homologous virus but no such association was found for HHV6 DNAemia. However, patients with consecutively positive DNAemia to any of the viruses (including HHV6) were more likely to have a homologous serological response. Patients who had detectable CMV IgM without a concurrent rise in CMV antibodies were significantly less likely to have CMV DNAemia (odds ratio = 0.16; 95% Cl 0.02-0.9). CMV IgM antibodies may be associated with HHV6 or HHV7 DNAemia (odds ratio 2.3; 95% Cl 0.5-15). This serological profile may reflect a cross-reactive response to HHV6, HHV7 or other herpesviruses. CMV IgM should not be used in isolation for the diagnosis of CMV infection or disease in this group of patients.
Persistent Identifierhttp://hdl.handle.net/10722/179758
ISSN
2015 Impact Factor: 1.998
2015 SCImago Journal Rankings: 1.015
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorOsman, HKen_US
dc.contributor.authorPeiris, JSMen_US
dc.contributor.authorTaylor, CEen_US
dc.contributor.authorKarlberg, JPEen_US
dc.contributor.authorMadeley, CRen_US
dc.date.accessioned2012-12-19T10:04:22Z-
dc.date.available2012-12-19T10:04:22Z-
dc.date.issued1997en_US
dc.identifier.citationJournal of Medical Virology, 1997, v. 53 n. 3, p. 288-294en_US
dc.identifier.issn0146-6615en_US
dc.identifier.urihttp://hdl.handle.net/10722/179758-
dc.description.abstractDiagnosis of significant infections by human herpesvirus 6 (HHV6) AND 7 (HHV7) in transplant patients has proved difficult because both viruses are ubiquitous and can cause persistent infections in their hosts. The significance of viral DNA detected in peripheral blood leukocytes (PBLs; DNAemial) by PCR is therefore unclear. The interpretation of serological results is complicated by the fact that both primary and secondary infections with other herpesviruses may be associated with a concurrent antibody response to HHV6. Fifty-four renal allograft recipients were studied prospectively and their serological response to HHV6, HHV7 and CMV were compared with the detection of viral DNAemia from the homologous heterologous viruses. Serum and heparinised blood samples were collected prospectively from 54 real allograft recipients. DNA was extracted from PBLs and tested for the presence of HHV6, HHV7 and CMV DNA by PCR. Antibodies to HHV6 and HHV7 were measured by an indirect immunofluorescence test and to CMV by an anticomplement immunofluorescence (ACIF) test. CMV IgM antibodies were detected by a commercial enzyme immunoassay. CMV and HHV7 DNAemia were each significantly associated with serological responses to the homologous virus but no such association was found for HHV6 DNAemia. However, patients with consecutively positive DNAemia to any of the viruses (including HHV6) were more likely to have a homologous serological response. Patients who had detectable CMV IgM without a concurrent rise in CMV antibodies were significantly less likely to have CMV DNAemia (odds ratio = 0.16; 95% Cl 0.02-0.9). CMV IgM antibodies may be associated with HHV6 or HHV7 DNAemia (odds ratio 2.3; 95% Cl 0.5-15). This serological profile may reflect a cross-reactive response to HHV6, HHV7 or other herpesviruses. CMV IgM should not be used in isolation for the diagnosis of CMV infection or disease in this group of patients.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763en_US
dc.relation.ispartofJournal of Medical Virologyen_US
dc.rightsJournal of Medical Virology. Copyright © John Wiley & Sons, Inc.-
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAntibodies, Viral - Blooden_US
dc.subject.meshChilden_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshCytomegalovirus - Genetics - Immunologyen_US
dc.subject.meshDna, Viral - Blooden_US
dc.subject.meshFemaleen_US
dc.subject.meshHerpesviridae Infections - Blood - Immunology - Virologyen_US
dc.subject.meshHerpesvirus 6, Human - Genetics - Immunologyen_US
dc.subject.meshHerpesvirus 7, Human - Genetics - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshKidney Transplantation - Adverse Effectsen_US
dc.subject.meshLeukocytes, Mononuclear - Immunology - Virologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPolymerase Chain Reaction - Methodsen_US
dc.subject.meshProspective Studiesen_US
dc.subject.meshTransplantation, Homologousen_US
dc.titleCorrelation between the detection of viral DNA by the polymerase chain reaction in peripheral blood leukocytes and serological responses to human herpesvirus 6, human herpesvirus 7, and cytomegalovirus in renal allograft recipientsen_US
dc.typeArticleen_US
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_US
dc.identifier.emailKarlberg, JPE: jpekarl@hkucc.hku.hken_US
dc.identifier.authorityPeiris, JSM=rp00410en_US
dc.identifier.authorityKarlberg, JPE=rp00400en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/(SICI)1096-9071(199711)53:3<288::AID-JMV19>3.0.CO;2-Den_US
dc.identifier.pmid9365898-
dc.identifier.scopuseid_2-s2.0-0031411491en_US
dc.identifier.hkuros26689-
dc.identifier.hkuros29881-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031411491&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume53en_US
dc.identifier.issue3en_US
dc.identifier.spage288en_US
dc.identifier.epage294en_US
dc.identifier.isiWOS:A1997YE75500019-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridOsman, HK=7005733119en_US
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_US
dc.identifier.scopusauthoridTaylor, CE=7404822545en_US
dc.identifier.scopusauthoridKarlberg, JPE=7005218406en_US
dc.identifier.scopusauthoridMadeley, CR=7006274504en_US

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