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Article: Role of target organ infection with cytomegalovirus in the pathogenesis of graft-versus-host disease

TitleRole of target organ infection with cytomegalovirus in the pathogenesis of graft-versus-host disease
Authors
Issue Date1995
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/bmt
Citation
Bone Marrow Transplantation, 1995, v. 15 n. 4, p. 557-561 How to Cite?
AbstractSkin and rectal biopsy tissue from 34 allogeneic and 23 autologous BMT recipients was prospectively analysed for CMV using immunohistochemistry and PCR to investigate the hypothesis that target organ infection with CMV initiates and/or exacerbates GVHD. Biopsies were obtained prior to and at 3, 8 and 26 weeks after BMT and whenever GVHD was suspected. Surveillance specimens of peripheral blood leucocytes (PBL), urine and throat swabs were obtained every 2 weeks until 12 weeks after BMT, and whenever CMV was suspected. Cryostat sections were analysed immunohistochemically for CMV antigens and PBL and biopsies for CMV DNA by PCR. Of the 31 patients who engrafted, 28 (90%) developed GVHD clinically, confirmed histologically in 56 biopsies. GVHD proved clinically severe in 14 patients, 4 of whom had treatment-resistant GVHD. CMV was detected in PBL more frequently in patients with severe GVHD than in those with mild/moderate GVHD (29% vs. 7%). However, in all but one patient the onset of GVHD preceded detection of CMV. In biopsy specimens, CMV was detected in only 2 patients, 1 of whom had an exacerbation of GVHD temporally associated with CMV. Thus, despite a high incidence of GVHD in this series, with 56 episodes of GVHD in 28 patients, only 1 patient had CMV in biopsy tissue temporally associated with GVHD. This suggests that biopsy infection with CMV is not a major factor in initiating or exacerbating GVHD in this cohort. This study thus does not support a role for target organ infection with CMV in the pathogenesis of GVHD.
Persistent Identifierhttp://hdl.handle.net/10722/179755
ISSN
2015 Impact Factor: 3.636
2015 SCImago Journal Rankings: 1.585
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorAppleton, ALen_US
dc.contributor.authorSviland, Len_US
dc.contributor.authorPeiris, JSMen_US
dc.contributor.authorTaylor, CEen_US
dc.contributor.authorWilkes, Jen_US
dc.contributor.authorGreen, MAen_US
dc.contributor.authorPearson, ADJen_US
dc.contributor.authorProctor, SJen_US
dc.contributor.authorHamilton, PJen_US
dc.contributor.authorCant, AJen_US
dc.contributor.authorMalcolm, AJen_US
dc.date.accessioned2012-12-19T10:04:21Z-
dc.date.available2012-12-19T10:04:21Z-
dc.date.issued1995en_US
dc.identifier.citationBone Marrow Transplantation, 1995, v. 15 n. 4, p. 557-561en_US
dc.identifier.issn0268-3369en_US
dc.identifier.urihttp://hdl.handle.net/10722/179755-
dc.description.abstractSkin and rectal biopsy tissue from 34 allogeneic and 23 autologous BMT recipients was prospectively analysed for CMV using immunohistochemistry and PCR to investigate the hypothesis that target organ infection with CMV initiates and/or exacerbates GVHD. Biopsies were obtained prior to and at 3, 8 and 26 weeks after BMT and whenever GVHD was suspected. Surveillance specimens of peripheral blood leucocytes (PBL), urine and throat swabs were obtained every 2 weeks until 12 weeks after BMT, and whenever CMV was suspected. Cryostat sections were analysed immunohistochemically for CMV antigens and PBL and biopsies for CMV DNA by PCR. Of the 31 patients who engrafted, 28 (90%) developed GVHD clinically, confirmed histologically in 56 biopsies. GVHD proved clinically severe in 14 patients, 4 of whom had treatment-resistant GVHD. CMV was detected in PBL more frequently in patients with severe GVHD than in those with mild/moderate GVHD (29% vs. 7%). However, in all but one patient the onset of GVHD preceded detection of CMV. In biopsy specimens, CMV was detected in only 2 patients, 1 of whom had an exacerbation of GVHD temporally associated with CMV. Thus, despite a high incidence of GVHD in this series, with 56 episodes of GVHD in 28 patients, only 1 patient had CMV in biopsy tissue temporally associated with GVHD. This suggests that biopsy infection with CMV is not a major factor in initiating or exacerbating GVHD in this cohort. This study thus does not support a role for target organ infection with CMV in the pathogenesis of GVHD.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/bmten_US
dc.relation.ispartofBone Marrow Transplantationen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshBiopsyen_US
dc.subject.meshBone Marrow Transplantationen_US
dc.subject.meshChilden_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshCytomegalovirus - Isolation & Purificationen_US
dc.subject.meshCytomegalovirus Infections - Diagnosisen_US
dc.subject.meshDna, Viral - Analysisen_US
dc.subject.meshFemaleen_US
dc.subject.meshGraft Vs Host Disease - Virologyen_US
dc.subject.meshHematologic Diseases - Therapyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoenzyme Techniquesen_US
dc.subject.meshInfanten_US
dc.subject.meshLeukocytes - Virologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshProspective Studiesen_US
dc.subject.meshRectum - Virologyen_US
dc.subject.meshSkin - Virologyen_US
dc.subject.meshTransplantation, Autologousen_US
dc.subject.meshTransplantation, Homologousen_US
dc.titleRole of target organ infection with cytomegalovirus in the pathogenesis of graft-versus-host diseaseen_US
dc.typeArticleen_US
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_US
dc.identifier.authorityPeiris, JSM=rp00410en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7655381-
dc.identifier.scopuseid_2-s2.0-0029022861en_US
dc.identifier.volume15en_US
dc.identifier.issue4en_US
dc.identifier.spage557en_US
dc.identifier.epage561en_US
dc.identifier.isiWOS:A1995RB31900011-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridAppleton, AL=7005827700en_US
dc.identifier.scopusauthoridSviland, L=7003823044en_US
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_US
dc.identifier.scopusauthoridTaylor, CE=7404822545en_US
dc.identifier.scopusauthoridWilkes, J=7102513218en_US
dc.identifier.scopusauthoridGreen, MA=16143678900en_US
dc.identifier.scopusauthoridPearson, ADJ=7401994312en_US
dc.identifier.scopusauthoridProctor, SJ=7102137191en_US
dc.identifier.scopusauthoridHamilton, PJ=35554560400en_US
dc.identifier.scopusauthoridCant, AJ=7006244214en_US
dc.identifier.scopusauthoridMalcolm, AJ=7103265161en_US

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