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Article: Transmission blocking immunity in Plasmodium vivax malaria: Antibodies raised against a peptide block parasite development in the mosquito vector

TitleTransmission blocking immunity in Plasmodium vivax malaria: Antibodies raised against a peptide block parasite development in the mosquito vector
Authors
Issue Date1995
PublisherRockefeller University Press. The Journal's web site is located at http://www.jem.org
Citation
Journal Of Experimental Medicine, 1995, v. 181 n. 1, p. 357-362 How to Cite?
AbstractOne approach towards the development of a vaccine against malaria is to immunize against the parasite sexual stages that mediate transmission of the parasite from man to mosquito. Antibodies against these stages, ingested with the blood meal, inhibit the parasite development in the mosquito vector, constituting 'transmission blocking immunity.' Most epitopes involved in transmission-blocking immunity depend on the tertiary conformational structure of surface antigens. However, one of the transmission-blocking monoclonal antibodies we have raised against Plasmodium vivax reacts with a linear epitope on both asexual stages and gametes. This monoclonal antibody (A12) is capable of totally blocking development of the parasite in the mosquito host when tested in membrane feeding assays with gametocytes from P. vivax-infected patients. Immune screening of a P. vivax λgt11 genomic expression library with A12 led to the isolation of a clone to which was mapped the six-amino acid epitope recognized by A12. Antisera raised in mice against a 12-mer synthetic peptide containing this epitope coupled to bovine serum albumin not only had high titers of antipeptide antibodies as measured by enzyme-linked immunosorbent assay, but in addition recognized the same 24- and 57-kD parasite components as A12 on Western blots and reacted with the parasite by immunofluorescence. When tested in membrane feeding assays, these antibodies have significant suppressive effects on parasite development in the mosquito.
Persistent Identifierhttp://hdl.handle.net/10722/179754
ISSN
2015 Impact Factor: 11.24
2015 SCImago Journal Rankings: 10.762
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSnewin, VAen_US
dc.contributor.authorPremawansa, Sen_US
dc.contributor.authorKapilananda, GMGen_US
dc.contributor.authorRatnayaka, Len_US
dc.contributor.authorUdagama, PVen_US
dc.contributor.authorMattei, DMen_US
dc.contributor.authorKhouri, Een_US
dc.contributor.authorDel Giudice, Gen_US
dc.contributor.authorPeiris, JSMen_US
dc.contributor.authorMendis, KNen_US
dc.contributor.authorDavid, PHen_US
dc.date.accessioned2012-12-19T10:04:20Z-
dc.date.available2012-12-19T10:04:20Z-
dc.date.issued1995en_US
dc.identifier.citationJournal Of Experimental Medicine, 1995, v. 181 n. 1, p. 357-362en_US
dc.identifier.issn0022-1007en_US
dc.identifier.urihttp://hdl.handle.net/10722/179754-
dc.description.abstractOne approach towards the development of a vaccine against malaria is to immunize against the parasite sexual stages that mediate transmission of the parasite from man to mosquito. Antibodies against these stages, ingested with the blood meal, inhibit the parasite development in the mosquito vector, constituting 'transmission blocking immunity.' Most epitopes involved in transmission-blocking immunity depend on the tertiary conformational structure of surface antigens. However, one of the transmission-blocking monoclonal antibodies we have raised against Plasmodium vivax reacts with a linear epitope on both asexual stages and gametes. This monoclonal antibody (A12) is capable of totally blocking development of the parasite in the mosquito host when tested in membrane feeding assays with gametocytes from P. vivax-infected patients. Immune screening of a P. vivax λgt11 genomic expression library with A12 led to the isolation of a clone to which was mapped the six-amino acid epitope recognized by A12. Antisera raised in mice against a 12-mer synthetic peptide containing this epitope coupled to bovine serum albumin not only had high titers of antipeptide antibodies as measured by enzyme-linked immunosorbent assay, but in addition recognized the same 24- and 57-kD parasite components as A12 on Western blots and reacted with the parasite by immunofluorescence. When tested in membrane feeding assays, these antibodies have significant suppressive effects on parasite development in the mosquito.en_US
dc.languageengen_US
dc.publisherRockefeller University Press. The Journal's web site is located at http://www.jem.orgen_US
dc.relation.ispartofJournal of Experimental Medicineen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonal - Immunologyen_US
dc.subject.meshEpitope Mappingen_US
dc.subject.meshMalaria Vaccines - Immunologyen_US
dc.subject.meshMalaria, Vivax - Prevention & Control - Transmissionen_US
dc.subject.meshMiceen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPeptides - Immunologyen_US
dc.subject.meshPlasmodium Vivax - Immunologyen_US
dc.subject.meshProtozoan Proteinsen_US
dc.subject.meshProtozoan Vaccines - Immunologyen_US
dc.titleTransmission blocking immunity in Plasmodium vivax malaria: Antibodies raised against a peptide block parasite development in the mosquito vectoren_US
dc.typeArticleen_US
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_US
dc.identifier.authorityPeiris, JSM=rp00410en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1084/jem.181.1.357en_US
dc.identifier.pmid7807016-
dc.identifier.scopuseid_2-s2.0-0028917889en_US
dc.identifier.volume181en_US
dc.identifier.issue1en_US
dc.identifier.spage357en_US
dc.identifier.epage362en_US
dc.identifier.isiWOS:A1995QA04200036-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSnewin, VA=6603553154en_US
dc.identifier.scopusauthoridPremawansa, S=6601964639en_US
dc.identifier.scopusauthoridKapilananda, GMG=6505733426en_US
dc.identifier.scopusauthoridRatnayaka, L=16172246400en_US
dc.identifier.scopusauthoridUdagama, PV=6505767945en_US
dc.identifier.scopusauthoridMattei, DM=7004315464en_US
dc.identifier.scopusauthoridKhouri, E=16166382700en_US
dc.identifier.scopusauthoridDel Giudice, G=7006629475en_US
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_US
dc.identifier.scopusauthoridMendis, KN=7004958149en_US
dc.identifier.scopusauthoridDavid, PH=7201509648en_US

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