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Article: Human herpes virus 6 and endogenous biotin in salivary glands

TitleHuman herpes virus 6 and endogenous biotin in salivary glands
Authors
Issue Date1992
PublisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
Citation
Journal Of Clinical Pathology, 1992, v. 45 n. 9, p. 788-790 How to Cite?
AbstractAims: To detect the presence of human herpes virus 6 (HHV6) and endogenous biotin in paraffin wax embedded and frozen salivary glands. Methods: Two stage indirect and streptavidin-biotin immunoperoxidase techniques were used to visualise the antigens. Results: HHV6 could not be shown in any of the tissues. However, considerable endogenous biotin antigenicity was detected in the glandular elements of the paraffin wax embedded material. Conclusions: Results obtained with avidin-biotin detection systems should be interpreted with caution, especially when glandular epithelium is being stained. This may apply to both immunoperoxidase and in situ hybridisation techniques. The use of an anti-biotin antibody as a standard control should be considered.
Persistent Identifierhttp://hdl.handle.net/10722/179743
ISSN
2015 Impact Factor: 2.912
2015 SCImago Journal Rankings: 1.260
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGreen, Men_US
dc.contributor.authorSviland, Len_US
dc.contributor.authorTaylor, CEen_US
dc.contributor.authorPeiris, Men_US
dc.contributor.authorMccarthy, ALen_US
dc.contributor.authorPearson, ADJen_US
dc.contributor.authorMalcolm, AJen_US
dc.date.accessioned2012-12-19T10:04:14Z-
dc.date.available2012-12-19T10:04:14Z-
dc.date.issued1992en_US
dc.identifier.citationJournal Of Clinical Pathology, 1992, v. 45 n. 9, p. 788-790en_US
dc.identifier.issn0021-9746en_US
dc.identifier.urihttp://hdl.handle.net/10722/179743-
dc.description.abstractAims: To detect the presence of human herpes virus 6 (HHV6) and endogenous biotin in paraffin wax embedded and frozen salivary glands. Methods: Two stage indirect and streptavidin-biotin immunoperoxidase techniques were used to visualise the antigens. Results: HHV6 could not be shown in any of the tissues. However, considerable endogenous biotin antigenicity was detected in the glandular elements of the paraffin wax embedded material. Conclusions: Results obtained with avidin-biotin detection systems should be interpreted with caution, especially when glandular epithelium is being stained. This may apply to both immunoperoxidase and in situ hybridisation techniques. The use of an anti-biotin antibody as a standard control should be considered.en_US
dc.languageengen_US
dc.publisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/en_US
dc.relation.ispartofJournal of Clinical Pathologyen_US
dc.subject.meshBiotin - Analysisen_US
dc.subject.meshCell Nucleus - Chemistryen_US
dc.subject.meshCytoplasm - Chemistryen_US
dc.subject.meshFrozen Sectionsen_US
dc.subject.meshHerpesvirus 6, Human - Isolation & Purificationen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoenzyme Techniquesen_US
dc.subject.meshParaffin Embedding - Methodsen_US
dc.subject.meshSalivary Glands - Chemistry - Microbiologyen_US
dc.titleHuman herpes virus 6 and endogenous biotin in salivary glandsen_US
dc.typeArticleen_US
dc.identifier.emailPeiris, M: malik@hkucc.hku.hken_US
dc.identifier.authorityPeiris, M=rp00410en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1136/jcp.45.9.788-
dc.identifier.pmid1328329-
dc.identifier.scopuseid_2-s2.0-0026686648en_US
dc.identifier.volume45en_US
dc.identifier.issue9en_US
dc.identifier.spage788en_US
dc.identifier.epage790en_US
dc.identifier.isiWOS:A1992JM51800011-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridGreen, M=16143678900en_US
dc.identifier.scopusauthoridSviland, L=7003823044en_US
dc.identifier.scopusauthoridTaylor, CE=7404822545en_US
dc.identifier.scopusauthoridPeiris, M=7005486823en_US
dc.identifier.scopusauthoridMcCarthy, AL=7102038738en_US
dc.identifier.scopusauthoridPearson, ADJ=7401994312en_US
dc.identifier.scopusauthoridMalcolm, AJ=7103265161en_US

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