File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Monoclonal-anti-Fc receptor IgG blocks antibody enhancement of viral replication in macrophages

TitleMonoclonal-anti-Fc receptor IgG blocks antibody enhancement of viral replication in macrophages
Authors
Issue Date1981
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/nature
Citation
Nature, 1981, v. 289 n. 5794, p. 189-191 How to Cite?
AbstractFlaviviruses, when complexed with antibody at subneutralizing concentrations, show enhanced replication in human and simian peripheral blood leukocytes and in P388 D1 and other macrophage cell lines. A comparable phenomenon has been demonstrated with alphaviruses and Bunyaviruses in P388 D1 cells, but cells lacking macrophage characteristics fail to show antibody-dependent enhancement (ADE) of viral replication. It has been suggested that the macrophage Fc receptor (FcR) provides an efficient route of entry of virus through the attachment of non-neutralized virus-antibody complexes and that for those viruses that escape destruction by the phagocyte, antibody results in a paradoxical increase in virus replication. Wst Nile virus (WNV) replication in the P388 D1 macrophage cell line provides a reproducible model system for studying ADE of viral replication. Mouse macrophages have two FcRs-FcR1, which is trypsin-sensitive and binds IgG2a, and FcRII, which is trypsin-resistant and binds IgG2b and IgG1 complexes. The FcR has been purified using rat anti-mouse FcR monoclonal antibody which blocks FcRII. We show here that anti-FcR IgG and its Fab fragment block ADE of virus replication by anti-WNV monoclonal antibodies.
Persistent Identifierhttp://hdl.handle.net/10722/179728
ISSN
2015 Impact Factor: 38.138
2015 SCImago Journal Rankings: 21.936
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorPeiris, JSMen_US
dc.contributor.authorGordon, Sen_US
dc.contributor.authorUnkeless, JCen_US
dc.contributor.authorPorterfield, JSen_US
dc.date.accessioned2012-12-19T10:04:06Z-
dc.date.available2012-12-19T10:04:06Z-
dc.date.issued1981en_US
dc.identifier.citationNature, 1981, v. 289 n. 5794, p. 189-191en_US
dc.identifier.issn0028-0836en_US
dc.identifier.urihttp://hdl.handle.net/10722/179728-
dc.description.abstractFlaviviruses, when complexed with antibody at subneutralizing concentrations, show enhanced replication in human and simian peripheral blood leukocytes and in P388 D1 and other macrophage cell lines. A comparable phenomenon has been demonstrated with alphaviruses and Bunyaviruses in P388 D1 cells, but cells lacking macrophage characteristics fail to show antibody-dependent enhancement (ADE) of viral replication. It has been suggested that the macrophage Fc receptor (FcR) provides an efficient route of entry of virus through the attachment of non-neutralized virus-antibody complexes and that for those viruses that escape destruction by the phagocyte, antibody results in a paradoxical increase in virus replication. Wst Nile virus (WNV) replication in the P388 D1 macrophage cell line provides a reproducible model system for studying ADE of viral replication. Mouse macrophages have two FcRs-FcR1, which is trypsin-sensitive and binds IgG2a, and FcRII, which is trypsin-resistant and binds IgG2b and IgG1 complexes. The FcR has been purified using rat anti-mouse FcR monoclonal antibody which blocks FcRII. We show here that anti-FcR IgG and its Fab fragment block ADE of virus replication by anti-WNV monoclonal antibodies.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/natureen_US
dc.relation.ispartofNatureen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Viralen_US
dc.subject.meshAntigen-Antibody Complexen_US
dc.subject.meshAntigens, Viralen_US
dc.subject.meshClone Cells - Immunologyen_US
dc.subject.meshImmunoglobulin Fab Fragmentsen_US
dc.subject.meshImmunoglobulin Gen_US
dc.subject.meshMacrophages - Immunology - Microbiologyen_US
dc.subject.meshMiceen_US
dc.subject.meshReceptors, Fc - Metabolismen_US
dc.subject.meshVirus Replicationen_US
dc.titleMonoclonal-anti-Fc receptor IgG blocks antibody enhancement of viral replication in macrophagesen_US
dc.typeArticleen_US
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_US
dc.identifier.authorityPeiris, JSM=rp00410en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/289189a0-
dc.identifier.pmid7453820-
dc.identifier.scopuseid_2-s2.0-0019831472en_US
dc.identifier.volume289en_US
dc.identifier.issue5794en_US
dc.identifier.spage189en_US
dc.identifier.epage191en_US
dc.identifier.isiWOS:A1981KY74600048-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_US
dc.identifier.scopusauthoridGordon, S=35391350600en_US
dc.identifier.scopusauthoridUnkeless, JC=7006626093en_US
dc.identifier.scopusauthoridPorterfield, JS=7005608715en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats