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Conference Paper: Reduction of chromate by marine bacillus megaterium TKW3

TitleReduction of chromate by marine bacillus megaterium TKW3
Authors
KeywordsBacillus Megaterium
Chromate Reductase
Corrosion Inhibitor
Heavy Metals
Proteomics
Issue Date2006
PublisherN A C E International. The Publisher's web site is located at http://www.nace.org/cstm/Store/
Citation
Nace - International Corrosion Conference Series, 2006, p. 065211-0652110 How to Cite?
AbstractHexavalent chromium (Cr6+) is an important corrosion inhibitor and is also highly toxic pollutant, which can be detoxified and precipitated through reduction to Cr3+. Bacillus megaterium TKW3 isolated from contaminated sediments was capable of reducing Cr6+ in concomitance with metalloids (Se4+, Se6+ and As5+). Notwithstanding the approximately 50% inhibition, it is firstly reported that bacteria reduced Cr6+ and Se4+ [to elemental Se] simultaneously. No significant difference was observed among electron donors (glucose, maltose and mannitol) on Cr6+ reduction of B. megaterium TKW3. The process was mediated by a novel aerobic membrane-associated reductase with Cr6+induced expression, and specific reductive activity of 0.220 nmol (Cr6+) mg-1 (protein) min-1. Respiratory inhibitor NaN3 did not obstruct the reductase activity. Transmission electron microscopy with energy dispersive X-ray analyzer confirmed the aggregation and accumulation of Cr along intracellular membrane region. 2-Dimensional gel electrophoresis analysis of the proteins expressed by B. megaterium TKW3 exposed to Cr6+ vs. that without Cr6+ in culture media revealed positive expression of specific proteins. Further identification of the amino acid sequence of this novel reductase would facilitate understanding of its enzymatic action on Cr6+ reduction and bioremediation. © 2006 by NACE International.
DescriptionCorrosion 2006 Conference, San Diego, California, United States, 12-16 March 2006. Paper No. 6521
Persistent Identifierhttp://hdl.handle.net/10722/179603
ISSN
2015 SCImago Journal Rankings: 0.101
References

 

DC FieldValueLanguage
dc.contributor.authorCheung, KHen_US
dc.contributor.authorLai, HYen_US
dc.contributor.authorGu, JDen_US
dc.date.accessioned2012-12-19T10:00:09Z-
dc.date.available2012-12-19T10:00:09Z-
dc.date.issued2006en_US
dc.identifier.citationNace - International Corrosion Conference Series, 2006, p. 065211-0652110en_US
dc.identifier.issn0361-4409en_US
dc.identifier.urihttp://hdl.handle.net/10722/179603-
dc.descriptionCorrosion 2006 Conference, San Diego, California, United States, 12-16 March 2006. Paper No. 6521-
dc.description.abstractHexavalent chromium (Cr6+) is an important corrosion inhibitor and is also highly toxic pollutant, which can be detoxified and precipitated through reduction to Cr3+. Bacillus megaterium TKW3 isolated from contaminated sediments was capable of reducing Cr6+ in concomitance with metalloids (Se4+, Se6+ and As5+). Notwithstanding the approximately 50% inhibition, it is firstly reported that bacteria reduced Cr6+ and Se4+ [to elemental Se] simultaneously. No significant difference was observed among electron donors (glucose, maltose and mannitol) on Cr6+ reduction of B. megaterium TKW3. The process was mediated by a novel aerobic membrane-associated reductase with Cr6+induced expression, and specific reductive activity of 0.220 nmol (Cr6+) mg-1 (protein) min-1. Respiratory inhibitor NaN3 did not obstruct the reductase activity. Transmission electron microscopy with energy dispersive X-ray analyzer confirmed the aggregation and accumulation of Cr along intracellular membrane region. 2-Dimensional gel electrophoresis analysis of the proteins expressed by B. megaterium TKW3 exposed to Cr6+ vs. that without Cr6+ in culture media revealed positive expression of specific proteins. Further identification of the amino acid sequence of this novel reductase would facilitate understanding of its enzymatic action on Cr6+ reduction and bioremediation. © 2006 by NACE International.en_US
dc.languageengen_US
dc.publisherN A C E International. The Publisher's web site is located at http://www.nace.org/cstm/Store/-
dc.relation.ispartofNACE - International Corrosion Conference Seriesen_US
dc.subjectBacillus Megateriumen_US
dc.subjectChromate Reductaseen_US
dc.subjectCorrosion Inhibitoren_US
dc.subjectHeavy Metalsen_US
dc.subjectProteomicsen_US
dc.titleReduction of chromate by marine bacillus megaterium TKW3en_US
dc.typeConference_Paperen_US
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_US
dc.identifier.authorityGu, JD=rp00701en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-69149093190en_US
dc.identifier.hkuros116805-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-69149093190&selection=ref&src=s&origin=recordpageen_US
dc.identifier.spage065211en_US
dc.identifier.epage0652110en_US
dc.identifier.scopusauthoridCheung, KH=7402406659en_US
dc.identifier.scopusauthoridLai, HY=15755593800en_US
dc.identifier.scopusauthoridGu, JD=7403129601en_US

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