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Conference Paper: Comparison of the QwikLite™ algal bioluminescence test with marine algal growth rate inhibition bioassays

TitleComparison of the QwikLite™ algal bioluminescence test with marine algal growth rate inhibition bioassays
Authors
Issue Date2008
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/10005202
Citation
Environmental Toxicology, 2008, v. 23 n. 5, p. 617-625 How to Cite?
AbstractAlthough marine algal bioassays based on growth rate inhibition over 72-96 h have been widely used to assess the toxicity of contaminants in waters and sediments, changes in pH over the test duration can lead to changes in contaminant speciation and consequently an under- or over-estimation of toxicity. In addition, high cell densities are used in order to obtain a detectable response, further reducing the tests' environmental relevance in marine waters. There is a need for rapid acute tests with ecologically relevant test endpoints that may be used as surrogates for longer-term chronic tests. This study compares the sensitivity and reproducibility of a rapid marine dinoflagellate (Pyrocystis lunula) bioluminescence test (QwikLite™) with standard algal growth rate bioassays (Nitzschia closterium and Entomoneis cf. punctulata) using ammonia and several antifouling agents (tributyltin [TBT], copper, and diuron) as reference toxicants. QwikLite was of similar sensitivity to ammonia as standard algal growth rate tests, but was less sensitive to copper, diuron and TBT, with 24-h EC50 values of 10 ± 1.1 mg N/L, 0.128 ± 0.021 mg Cu/L, 19 ± 13 mg diuron/L, and 0.226 ± 0.028 mg TBT/L. Inter-test precision using different batches of P. lunula was generally acceptable. On the basis of NOEC values, QwikLite™ was more sensitive to copper and ammonia at 25°C than at 21°. QwikLite™ shows promise as a rapid, inexpensive screening test for acute toxicity of contaminants in marine environments. © 2008 Wiley Periodicals, Inc.
DescriptionThe issue is a Special issue: 13th International Symposium on Toxicology Assessment
Persistent Identifierhttp://hdl.handle.net/10722/179601
ISSN
2015 Impact Factor: 2.868
2015 SCImago Journal Rankings: 0.819
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorStauber, JLen_US
dc.contributor.authorBinet, MTen_US
dc.contributor.authorBao, VWWen_US
dc.contributor.authorBoge, Jen_US
dc.contributor.authorZhang, AQen_US
dc.contributor.authorLeung, KMYen_US
dc.contributor.authorAdams, MSen_US
dc.date.accessioned2012-12-19T10:00:08Z-
dc.date.available2012-12-19T10:00:08Z-
dc.date.issued2008en_US
dc.identifier.citationEnvironmental Toxicology, 2008, v. 23 n. 5, p. 617-625en_US
dc.identifier.issn1520-4081en_US
dc.identifier.urihttp://hdl.handle.net/10722/179601-
dc.descriptionThe issue is a Special issue: 13th International Symposium on Toxicology Assessment-
dc.description.abstractAlthough marine algal bioassays based on growth rate inhibition over 72-96 h have been widely used to assess the toxicity of contaminants in waters and sediments, changes in pH over the test duration can lead to changes in contaminant speciation and consequently an under- or over-estimation of toxicity. In addition, high cell densities are used in order to obtain a detectable response, further reducing the tests' environmental relevance in marine waters. There is a need for rapid acute tests with ecologically relevant test endpoints that may be used as surrogates for longer-term chronic tests. This study compares the sensitivity and reproducibility of a rapid marine dinoflagellate (Pyrocystis lunula) bioluminescence test (QwikLite™) with standard algal growth rate bioassays (Nitzschia closterium and Entomoneis cf. punctulata) using ammonia and several antifouling agents (tributyltin [TBT], copper, and diuron) as reference toxicants. QwikLite was of similar sensitivity to ammonia as standard algal growth rate tests, but was less sensitive to copper, diuron and TBT, with 24-h EC50 values of 10 ± 1.1 mg N/L, 0.128 ± 0.021 mg Cu/L, 19 ± 13 mg diuron/L, and 0.226 ± 0.028 mg TBT/L. Inter-test precision using different batches of P. lunula was generally acceptable. On the basis of NOEC values, QwikLite™ was more sensitive to copper and ammonia at 25°C than at 21°. QwikLite™ shows promise as a rapid, inexpensive screening test for acute toxicity of contaminants in marine environments. © 2008 Wiley Periodicals, Inc.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/10005202en_US
dc.relation.ispartofEnvironmental Toxicologyen_US
dc.rightsEnvironmental Toxicology. Copyright © John Wiley & Sons, Inc.-
dc.subject.meshAmmonia - Toxicityen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiological Assay - Methodsen_US
dc.subject.meshCopper - Toxicityen_US
dc.subject.meshDiatoms - Drug Effects - Growth & Developmenten_US
dc.subject.meshDinoflagellida - Drug Effects - Growth & Developmenten_US
dc.subject.meshDiuron - Toxicityen_US
dc.subject.meshEnvironmental Monitoring - Methodsen_US
dc.subject.meshLuminescent Measurementsen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.subject.meshTrialkyltin Compounds - Toxicityen_US
dc.subject.meshWater Pollutants, Chemical - Toxicityen_US
dc.titleComparison of the QwikLite™ algal bioluminescence test with marine algal growth rate inhibition bioassaysen_US
dc.typeConference_Paperen_US
dc.identifier.emailLeung, KMY: kmyleung@hku.hken_US
dc.identifier.authorityLeung, KMY=rp00733en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/tox.20400en_US
dc.identifier.pmid18528914-
dc.identifier.scopuseid_2-s2.0-51949085366en_US
dc.identifier.hkuros154255-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-51949085366&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume23en_US
dc.identifier.issue5en_US
dc.identifier.spage617en_US
dc.identifier.epage625en_US
dc.identifier.isiWOS:000259284000009-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridStauber, JL=7004542809en_US
dc.identifier.scopusauthoridBinet, MT=7003485267en_US
dc.identifier.scopusauthoridBao, VWW=23666277800en_US
dc.identifier.scopusauthoridBoge, J=24828960500en_US
dc.identifier.scopusauthoridZhang, AQ=23669449500en_US
dc.identifier.scopusauthoridLeung, KMY=7401860738en_US
dc.identifier.scopusauthoridAdams, MS=7403905462en_US

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