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Conference Paper: Determination of vitamin B 1 in seawater and microalgal fermentation media by high-performance liquid chromatography with fluorescence detection

TitleDetermination of vitamin B 1 in seawater and microalgal fermentation media by high-performance liquid chromatography with fluorescence detection
Authors
KeywordsFermentation Media
High-Performance Liquid Chromatography
Seawater
Solid-Phase Extraction
Vitamin B 1
Issue Date2005
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htm
Citation
Analytical And Bioanalytical Chemistry, 2005, v. 383 n. 5, p. 875-879 How to Cite?
AbstractA highly sensitive high-performance liquid chromatographic method with fluorescence detection has been developed for determination of vitamin B 1. Vitamin B 1 was converted into a fluorescent compound by treatment with hydrogen peroxide-horseradish peroxidase and the derivative was subsequently analyzed by HPLC on a Waters Spherisorb ODS2 column (250 mm×4.6 mm ID, 5 μm) with 40:60 methanol-pH 8.5 acetate buffer solution as mobile phase and fluorescence detection at 440 nm (with excitation at 375 nm). The calibration graph was linear from 5.00×10 -10 mol L -1 to 5.00×10 -7 mol L -1 for vitamin B 1 with a correlation coefficient of 0.9991 (n=9). The detection limit was 1.0×10 -10 mol L -1. The method was successfully used for determination of vitamin B 1 at pg mL -1 levels in microalgal fermentation media and seawater after solid-phase extraction. Recovery was from 89 to 110% and the relative standard deviation was in the range 1.1 to 4.3%. © Springer-Verlag 2005.
Persistent Identifierhttp://hdl.handle.net/10722/179589
ISSN
2015 Impact Factor: 3.125
2015 SCImago Journal Rankings: 1.096
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHe, HZen_US
dc.contributor.authorLi, HBen_US
dc.contributor.authorChen, Fen_US
dc.date.accessioned2012-12-19T10:00:03Z-
dc.date.available2012-12-19T10:00:03Z-
dc.date.issued2005en_US
dc.identifier.citationAnalytical And Bioanalytical Chemistry, 2005, v. 383 n. 5, p. 875-879en_US
dc.identifier.issn1618-2642en_US
dc.identifier.urihttp://hdl.handle.net/10722/179589-
dc.description.abstractA highly sensitive high-performance liquid chromatographic method with fluorescence detection has been developed for determination of vitamin B 1. Vitamin B 1 was converted into a fluorescent compound by treatment with hydrogen peroxide-horseradish peroxidase and the derivative was subsequently analyzed by HPLC on a Waters Spherisorb ODS2 column (250 mm×4.6 mm ID, 5 μm) with 40:60 methanol-pH 8.5 acetate buffer solution as mobile phase and fluorescence detection at 440 nm (with excitation at 375 nm). The calibration graph was linear from 5.00×10 -10 mol L -1 to 5.00×10 -7 mol L -1 for vitamin B 1 with a correlation coefficient of 0.9991 (n=9). The detection limit was 1.0×10 -10 mol L -1. The method was successfully used for determination of vitamin B 1 at pg mL -1 levels in microalgal fermentation media and seawater after solid-phase extraction. Recovery was from 89 to 110% and the relative standard deviation was in the range 1.1 to 4.3%. © Springer-Verlag 2005.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htmen_US
dc.relation.ispartofAnalytical and Bioanalytical Chemistryen_US
dc.subjectFermentation Mediaen_US
dc.subjectHigh-Performance Liquid Chromatographyen_US
dc.subjectSeawateren_US
dc.subjectSolid-Phase Extractionen_US
dc.subjectVitamin B 1en_US
dc.titleDetermination of vitamin B 1 in seawater and microalgal fermentation media by high-performance liquid chromatography with fluorescence detectionen_US
dc.typeConference_Paperen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00216-005-0062-1en_US
dc.identifier.pmid16172881-
dc.identifier.scopuseid_2-s2.0-27744522318en_US
dc.identifier.hkuros122501-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-27744522318&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume383en_US
dc.identifier.issue5en_US
dc.identifier.spage875en_US
dc.identifier.epage879en_US
dc.identifier.isiWOS:000233874900026-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridHe, HZ=55214936100en_US
dc.identifier.scopusauthoridLi, HB=25649944400en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US

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