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Conference Paper: Protein patterning in polycarbonate microfluidic channels

TitleProtein patterning in polycarbonate microfluidic channels
Authors
KeywordsBiomaterials
Excimer Laser
Polyethylene Oxide
Polymer Microfluidics
Protein Patterning
Surface Modification
Issue Date2004
PublisherS P I E - International Society for Optical Engineering. The Journal's web site is located at http://spie.org/x1848.xml
Citation
Proceedings Of Spie - The International Society For Optical Engineering, 2004, v. 5275, p. 161-167 How to Cite?
AbstractIn this work protein patterning has been demonstrated within a polycarbonate microfluidic device. Channel structures were first coated by plasma polymerization of allylamine (ALAPP) followed by 'cloud point' grafting of polyethylene oxide (PEO), resulting in a protein repellent surface. Excimer laser micromachining was used to pattern the PEO to control protein localisation. Subsequent removal of a sacrificial layer of polycarbonate resulted in the patterned polymer coating only within the channels of a simple fluidic device. Following a final diffusion bonding fabrication step the devices were filled with a buffer containing streptavidin conjugated with fluorescein, and visualized under a confocal fluorescence microscope. This confirmed that protein adhesion occurred only in laser-patterned areas. The ability to control protein adhesion in microfluidic channels leads to the possibility of generating arrays of proteins or cells within polymer microfluidics for cheap automated biosensors and synthesis systems.
Persistent Identifierhttp://hdl.handle.net/10722/179587
ISSN
References

 

DC FieldValueLanguage
dc.contributor.authorThomson, Den_US
dc.contributor.authorHayes, JPen_US
dc.contributor.authorThissen, Hen_US
dc.date.accessioned2012-12-19T10:00:02Z-
dc.date.available2012-12-19T10:00:02Z-
dc.date.issued2004en_US
dc.identifier.citationProceedings Of Spie - The International Society For Optical Engineering, 2004, v. 5275, p. 161-167en_US
dc.identifier.issn0277-786Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/179587-
dc.description.abstractIn this work protein patterning has been demonstrated within a polycarbonate microfluidic device. Channel structures were first coated by plasma polymerization of allylamine (ALAPP) followed by 'cloud point' grafting of polyethylene oxide (PEO), resulting in a protein repellent surface. Excimer laser micromachining was used to pattern the PEO to control protein localisation. Subsequent removal of a sacrificial layer of polycarbonate resulted in the patterned polymer coating only within the channels of a simple fluidic device. Following a final diffusion bonding fabrication step the devices were filled with a buffer containing streptavidin conjugated with fluorescein, and visualized under a confocal fluorescence microscope. This confirmed that protein adhesion occurred only in laser-patterned areas. The ability to control protein adhesion in microfluidic channels leads to the possibility of generating arrays of proteins or cells within polymer microfluidics for cheap automated biosensors and synthesis systems.en_US
dc.languageengen_US
dc.publisherS P I E - International Society for Optical Engineering. The Journal's web site is located at http://spie.org/x1848.xmlen_US
dc.relation.ispartofProceedings of SPIE - The International Society for Optical Engineeringen_US
dc.subjectBiomaterialsen_US
dc.subjectExcimer Laseren_US
dc.subjectPolyethylene Oxideen_US
dc.subjectPolymer Microfluidicsen_US
dc.subjectProtein Patterningen_US
dc.subjectSurface Modificationen_US
dc.titleProtein patterning in polycarbonate microfluidic channelsen_US
dc.typeConference_Paperen_US
dc.identifier.emailThomson, D: dthomson@hku.hken_US
dc.identifier.authorityThomson, D=rp00788en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1117/12.524675en_US
dc.identifier.scopuseid_2-s2.0-2442589520en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-2442589520&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume5275en_US
dc.identifier.spage161en_US
dc.identifier.epage167en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridThomson, D=7202586830en_US
dc.identifier.scopusauthoridHayes, JP=7403555100en_US
dc.identifier.scopusauthoridThissen, H=6701836823en_US

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