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Article: Isolation of a mitogenic agglutinin with relatively high thermostability from seeds of the variegated shell ginger

TitleIsolation of a mitogenic agglutinin with relatively high thermostability from seeds of the variegated shell ginger
Authors
Issue Date2010
PublisherBentham Science Publishers Ltd. The Journal's web site is located at http://www.bentham.org/ppl/index.htm
Citation
Protein And Peptide Letters, 2010, v. 17 n. 1, p. 38-43 How to Cite?
AbstractAn agglutinin with a molecular mass of 130 kDa has been isolated from the seeds of Alpinia zerumbet cv.'Variegata'. The isolation procedure involved anion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, and gel filtration by fast protein liquid chromatography on Superdex 75. The agglutinin exhibited hemagglutinating activity toward rabbit erythrocytes which could not be inhibited by simple sugars. It was composed of four identical 32-kDa subunits with substantial N-terminal sequence similarity to chitinase and yieldin. The hemagglutinating activity of A. zerumbet agglutinin was stable up to 80°C and not affected by presence of a variety of salts. The agglutinin stimulated [methyl- 3H]-thymidine uptake by mouse splenocytes. It did not exhibit antifungal activity. © 2010 Bentham Science Publishers Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/179455
ISSN
2015 Impact Factor: 1.069
2015 SCImago Journal Rankings: 0.472
References

 

DC FieldValueLanguage
dc.contributor.authorWong, JHen_US
dc.contributor.authorNg, TBen_US
dc.contributor.authorZhang, KYBen_US
dc.contributor.authorSze, SCWen_US
dc.contributor.authorWang, HXen_US
dc.date.accessioned2012-12-19T09:56:44Z-
dc.date.available2012-12-19T09:56:44Z-
dc.date.issued2010en_US
dc.identifier.citationProtein And Peptide Letters, 2010, v. 17 n. 1, p. 38-43en_US
dc.identifier.issn0929-8665en_US
dc.identifier.urihttp://hdl.handle.net/10722/179455-
dc.description.abstractAn agglutinin with a molecular mass of 130 kDa has been isolated from the seeds of Alpinia zerumbet cv.'Variegata'. The isolation procedure involved anion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, and gel filtration by fast protein liquid chromatography on Superdex 75. The agglutinin exhibited hemagglutinating activity toward rabbit erythrocytes which could not be inhibited by simple sugars. It was composed of four identical 32-kDa subunits with substantial N-terminal sequence similarity to chitinase and yieldin. The hemagglutinating activity of A. zerumbet agglutinin was stable up to 80°C and not affected by presence of a variety of salts. The agglutinin stimulated [methyl- 3H]-thymidine uptake by mouse splenocytes. It did not exhibit antifungal activity. © 2010 Bentham Science Publishers Ltd.en_US
dc.languageengen_US
dc.publisherBentham Science Publishers Ltd. The Journal's web site is located at http://www.bentham.org/ppl/index.htmen_US
dc.relation.ispartofProtein and Peptide Lettersen_US
dc.subject.meshAgglutinins - Chemistry - Genetics - Metabolism - Pharmacologyen_US
dc.subject.meshAlpinia - Chemistryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Growth Processes - Drug Effectsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshChromatography, Ion Exchange - Methodsen_US
dc.subject.meshMiceen_US
dc.subject.meshMycelium - Drug Effects - Growth & Developmenten_US
dc.subject.meshProtein Stabilityen_US
dc.subject.meshSeeds - Chemistryen_US
dc.titleIsolation of a mitogenic agglutinin with relatively high thermostability from seeds of the variegated shell gingeren_US
dc.typeArticleen_US
dc.identifier.emailZhang, KYB: ybzhang@hku.hken_US
dc.identifier.emailSze, SCW: stephens@hku.hken_US
dc.identifier.authorityZhang, KYB=rp01410en_US
dc.identifier.authoritySze, SCW=rp00514en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.2174/092986610789909539en_US
dc.identifier.pmid20214629-
dc.identifier.scopuseid_2-s2.0-77950013053en_US
dc.identifier.hkuros171310-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77950013053&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume17en_US
dc.identifier.issue1en_US
dc.identifier.spage38en_US
dc.identifier.epage43en_US
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridWong, JH=34874358200en_US
dc.identifier.scopusauthoridNg, TB=35311803300en_US
dc.identifier.scopusauthoridZhang, KYB=23483121900en_US
dc.identifier.scopusauthoridSze, SCW=23482617000en_US
dc.identifier.scopusauthoridWang, HX=7501734185en_US
dc.identifier.citeulike6204082-

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