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Article: Isolation and characterization of novel single-chain Fv specific for human granzyme B

TitleIsolation and characterization of novel single-chain Fv specific for human granzyme B
Authors
Issue Date2004
Citation
Hybridoma And Hybridomics, 2004, v. 23 n. 4, p. 219-231 How to Cite?
AbstractGranzyme B, a neutral serine protease, has been demonstrated to be a pivotal molecule for protective immunity against viral infection and cellular malignant transformation. To facilitate monitoring of granzyme B levels, we have recently applied phage display technology to produce single-chain Fv antibodies specific for granzyme B, as versatile alternatives and complementary reagents to currently available monoclonal antibodies. Through four rounds of panning on purified human granzyme B-coated on solid phase, three unique clones were isolated. Expressed soluble scFv antibodies demonstrated specific immunological applications including ELISA, Western blotting, immunoprecipitation and intracellular staining. Based on sequence analyses and structural modeling, one scFv, Fv17, may have overlapping antigen binding specificity with monoclonal antibodies 2C5/F5 and GB11. Owing to the availability of its DNA sequence and large scale production capability, Fv17 should be a superior reagent for monitoring granzyme B expression in natural killer cells and antigen specific CD8+ T cell immunity.
Persistent Identifierhttp://hdl.handle.net/10722/179444
ISSN
2006 Impact Factor: 0.559
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorRong, Jen_US
dc.contributor.authorXu, Xen_US
dc.contributor.authorEwen, Cen_US
dc.contributor.authorBleackley, RCen_US
dc.contributor.authorKane, KPen_US
dc.date.accessioned2012-12-19T09:56:38Z-
dc.date.available2012-12-19T09:56:38Z-
dc.date.issued2004en_US
dc.identifier.citationHybridoma And Hybridomics, 2004, v. 23 n. 4, p. 219-231en_US
dc.identifier.issn1536-8599en_US
dc.identifier.urihttp://hdl.handle.net/10722/179444-
dc.description.abstractGranzyme B, a neutral serine protease, has been demonstrated to be a pivotal molecule for protective immunity against viral infection and cellular malignant transformation. To facilitate monitoring of granzyme B levels, we have recently applied phage display technology to produce single-chain Fv antibodies specific for granzyme B, as versatile alternatives and complementary reagents to currently available monoclonal antibodies. Through four rounds of panning on purified human granzyme B-coated on solid phase, three unique clones were isolated. Expressed soluble scFv antibodies demonstrated specific immunological applications including ELISA, Western blotting, immunoprecipitation and intracellular staining. Based on sequence analyses and structural modeling, one scFv, Fv17, may have overlapping antigen binding specificity with monoclonal antibodies 2C5/F5 and GB11. Owing to the availability of its DNA sequence and large scale production capability, Fv17 should be a superior reagent for monitoring granzyme B expression in natural killer cells and antigen specific CD8+ T cell immunity.en_US
dc.languageengen_US
dc.relation.ispartofHybridoma and Hybridomicsen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAntibodies - Chemistry - Immunologyen_US
dc.subject.meshAntibody Specificityen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshDna Primersen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshGranzymesen_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshSequence Homology, Amino Aciden_US
dc.subject.meshSerine Endopeptidases - Immunologyen_US
dc.titleIsolation and characterization of novel single-chain Fv specific for human granzyme Ben_US
dc.typeArticleen_US
dc.identifier.emailRong, J: jrong@hku.hken_US
dc.identifier.authorityRong, J=rp00515en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1089/1536859041651349en_US
dc.identifier.pmid15319069-
dc.identifier.scopuseid_2-s2.0-4143093959en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-4143093959&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume23en_US
dc.identifier.issue4en_US
dc.identifier.spage219en_US
dc.identifier.epage231en_US
dc.identifier.isiWOS:000223323300003-
dc.identifier.scopusauthoridRong, J=7005980047en_US
dc.identifier.scopusauthoridXu, X=37082623100en_US
dc.identifier.scopusauthoridEwen, C=6603081465en_US
dc.identifier.scopusauthoridBleackley, RC=35477484000en_US
dc.identifier.scopusauthoridKane, KP=24534271300en_US

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