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Article: Characterization of steroidal saponins in crude extract from Dioscorea nipponica Makino by liquid chromatography tandem multi-stage mass spectrometry

TitleCharacterization of steroidal saponins in crude extract from Dioscorea nipponica Makino by liquid chromatography tandem multi-stage mass spectrometry
Authors
Issue Date2007
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aca
Citation
Analytica Chimica Acta, 2007, v. 599 n. 1, p. 98-106 How to Cite?
AbstractThe liquid chromatography-electrospray ionization-tandem multi-stage mass spectrometry (LC-ESI-MS n) method was developed for the analyses and characterization of steroidal saponins in plant extract from the rhizome of Dioscorea nipponica Makino. The HPLC experiments were performed by means of a reversed-phase C 18 column and a binary mobile phase system consisting of water and acetonitrile under gradient elution conditions. Pseudoprotodioscin, methyl protodioscin and dioscin were identified by comparing the retention times, UV spectra and the fragmentation properties of [M - H] - ions with the authentic standards. Four groups of steroidal saponin isomers possessed the [M - H] - ions at m/z 1063, 1045, 901 and 1047, respectively, were observed during the LC-ESI(-)-MS analysis, and three groups of them except the pair of isomers with the [M - H] - ions at m/z 1047 could be differentiated by LC-ESI(-)-MS 3. Furthermore, the ESI-MS n fragmentation behaviors of the [M + Li] + ions of pseudoprotodioscin and methyl protodioscin have been investigated, and the observed information helped the structural elucidation of the more abundant isomer with the [M - H] - ion at m/z 1047. As the result, a special sugar sequence of the saccharide chains was observed that not glucose but rhamnose might be connected with the hydroxyl group at C-3 position of the steroidal aglycone. © 2007 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/179440
ISSN
2015 Impact Factor: 4.712
2015 SCImago Journal Rankings: 1.548
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLin, Sen_US
dc.contributor.authorWang, Den_US
dc.contributor.authorYang, Den_US
dc.contributor.authorYao, Jen_US
dc.contributor.authorTong, Yen_US
dc.contributor.authorChen, Jen_US
dc.date.accessioned2012-12-19T09:56:36Z-
dc.date.available2012-12-19T09:56:36Z-
dc.date.issued2007en_US
dc.identifier.citationAnalytica Chimica Acta, 2007, v. 599 n. 1, p. 98-106en_US
dc.identifier.issn0003-2670en_US
dc.identifier.urihttp://hdl.handle.net/10722/179440-
dc.description.abstractThe liquid chromatography-electrospray ionization-tandem multi-stage mass spectrometry (LC-ESI-MS n) method was developed for the analyses and characterization of steroidal saponins in plant extract from the rhizome of Dioscorea nipponica Makino. The HPLC experiments were performed by means of a reversed-phase C 18 column and a binary mobile phase system consisting of water and acetonitrile under gradient elution conditions. Pseudoprotodioscin, methyl protodioscin and dioscin were identified by comparing the retention times, UV spectra and the fragmentation properties of [M - H] - ions with the authentic standards. Four groups of steroidal saponin isomers possessed the [M - H] - ions at m/z 1063, 1045, 901 and 1047, respectively, were observed during the LC-ESI(-)-MS analysis, and three groups of them except the pair of isomers with the [M - H] - ions at m/z 1047 could be differentiated by LC-ESI(-)-MS 3. Furthermore, the ESI-MS n fragmentation behaviors of the [M + Li] + ions of pseudoprotodioscin and methyl protodioscin have been investigated, and the observed information helped the structural elucidation of the more abundant isomer with the [M - H] - ion at m/z 1047. As the result, a special sugar sequence of the saccharide chains was observed that not glucose but rhamnose might be connected with the hydroxyl group at C-3 position of the steroidal aglycone. © 2007 Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/acaen_US
dc.relation.ispartofAnalytica Chimica Actaen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshDioscorea - Chemistryen_US
dc.subject.meshDiosgenin - Analogs & Derivatives - Analysisen_US
dc.subject.meshMethanol - Chemistryen_US
dc.subject.meshPlant Extracts - Analysisen_US
dc.subject.meshRhizome - Chemistryen_US
dc.subject.meshSaponins - Analysisen_US
dc.subject.meshSpectrometry, Mass, Electrospray Ionizationen_US
dc.subject.meshTandem Mass Spectrometryen_US
dc.titleCharacterization of steroidal saponins in crude extract from Dioscorea nipponica Makino by liquid chromatography tandem multi-stage mass spectrometryen_US
dc.typeArticleen_US
dc.identifier.emailTong, Y: tongyao@hku.hken_US
dc.identifier.emailChen, J: abchen@hku.hken_US
dc.identifier.authorityTong, Y=rp00509en_US
dc.identifier.authorityChen, J=rp01316en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.aca.2007.07.070en_US
dc.identifier.pmid17765069-
dc.identifier.scopuseid_2-s2.0-34548156161en_US
dc.identifier.hkuros133749-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34548156161&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume599en_US
dc.identifier.issue1en_US
dc.identifier.spage98en_US
dc.identifier.epage106en_US
dc.identifier.isiWOS:000249681800013-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridLin, S=36237034900en_US
dc.identifier.scopusauthoridWang, D=7407071911en_US
dc.identifier.scopusauthoridYang, D=8901049400en_US
dc.identifier.scopusauthoridYao, J=35560043800en_US
dc.identifier.scopusauthoridTong, Y=9045384000en_US
dc.identifier.scopusauthoridChen, J=22733695400en_US

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