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Article: Evaluating antigen-specific cytotoxic T lymphocyte responses by a novel mouse granzyme B ELISPOT assay

TitleEvaluating antigen-specific cytotoxic T lymphocyte responses by a novel mouse granzyme B ELISPOT assay
Authors
Issue Date2006
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jim
Citation
Journal Of Immunological Methods, 2006, v. 308 n. 1-2, p. 156-166 How to Cite?
AbstractWe have established novel ELISA- and ELISPOT-based assays specific for the detection of a potent cytotoxic mediator, granzyme B (GrB), for the assessment of antigen-specific cytotoxic T lymphocyte responses in mice. The sensitivity and specificity of our assays was demonstrated by ELISA using purified mouse GrB and supernatants and cell lysates of cytotoxic lymphocytes derived from GrB-deficient mice. No reactivity was observed by the GrB ELISA in GrB-deficient cells. The mouse GrB ELISPOT was successfully employed to detect antigen-specific effector cell responses of two CTL clones, producing GrB ELISPOT results that correlated strongly with target cell lysis, as assessed by 51Cr-release. Furthermore, we were able to demonstrate direct correlations between GrB ELISPOT and killing by LCMV gp33-specific effector and memory T cells generated in vivo. Thus, the mouse GrB ELISPOT may be used to detect cytotoxic responses, at the single-cell level, for the functional assessment of antigen-specific CD8+ T cell responses in mouse models of infection. © 2005 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/179429
ISSN
2015 Impact Factor: 1.858
2015 SCImago Journal Rankings: 1.053
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorEwen, CLen_US
dc.contributor.authorRong, Jen_US
dc.contributor.authorKokaji, AIen_US
dc.contributor.authorBleackley, RCen_US
dc.contributor.authorKane, KPen_US
dc.date.accessioned2012-12-19T09:56:25Z-
dc.date.available2012-12-19T09:56:25Z-
dc.date.issued2006en_US
dc.identifier.citationJournal Of Immunological Methods, 2006, v. 308 n. 1-2, p. 156-166en_US
dc.identifier.issn0022-1759en_US
dc.identifier.urihttp://hdl.handle.net/10722/179429-
dc.description.abstractWe have established novel ELISA- and ELISPOT-based assays specific for the detection of a potent cytotoxic mediator, granzyme B (GrB), for the assessment of antigen-specific cytotoxic T lymphocyte responses in mice. The sensitivity and specificity of our assays was demonstrated by ELISA using purified mouse GrB and supernatants and cell lysates of cytotoxic lymphocytes derived from GrB-deficient mice. No reactivity was observed by the GrB ELISA in GrB-deficient cells. The mouse GrB ELISPOT was successfully employed to detect antigen-specific effector cell responses of two CTL clones, producing GrB ELISPOT results that correlated strongly with target cell lysis, as assessed by 51Cr-release. Furthermore, we were able to demonstrate direct correlations between GrB ELISPOT and killing by LCMV gp33-specific effector and memory T cells generated in vivo. Thus, the mouse GrB ELISPOT may be used to detect cytotoxic responses, at the single-cell level, for the functional assessment of antigen-specific CD8+ T cell responses in mouse models of infection. © 2005 Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jimen_US
dc.relation.ispartofJournal of Immunological Methodsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Viralen_US
dc.subject.meshCell Degranulationen_US
dc.subject.meshCytotoxicity Tests, Immunologic - Methods - Statistics & Numerical Dataen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assay - Methods - Statistics & Numerical Dataen_US
dc.subject.meshFemaleen_US
dc.subject.meshGranzymesen_US
dc.subject.meshImmunologic Memoryen_US
dc.subject.meshLymphocytic Choriomeningitis Virus - Immunologyen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Balb Cen_US
dc.subject.meshMice, Inbred C57blen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshSerine Endopeptidases - Analysis - Biosynthesisen_US
dc.subject.meshT-Lymphocytes, Cytotoxic - Enzymology - Immunologyen_US
dc.titleEvaluating antigen-specific cytotoxic T lymphocyte responses by a novel mouse granzyme B ELISPOT assayen_US
dc.typeArticleen_US
dc.identifier.emailRong, J: jrong@hku.hken_US
dc.identifier.authorityRong, J=rp00515en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jim.2005.10.009en_US
dc.identifier.pmid16375915-
dc.identifier.scopuseid_2-s2.0-30944445218en_US
dc.identifier.hkuros130743-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-30944445218&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume308en_US
dc.identifier.issue1-2en_US
dc.identifier.spage156en_US
dc.identifier.epage166en_US
dc.identifier.isiWOS:000235285700015-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridEwen, CL=6603081465en_US
dc.identifier.scopusauthoridRong, J=7005980047en_US
dc.identifier.scopusauthoridKokaji, AI=8238711200en_US
dc.identifier.scopusauthoridBleackley, RC=35477484000en_US
dc.identifier.scopusauthoridKane, KP=24534271300en_US

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