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- Publisher Website: 10.1016/j.jim.2005.10.009
- Scopus: eid_2-s2.0-30944445218
- PMID: 16375915
- WOS: WOS:000235285700015
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Article: Evaluating antigen-specific cytotoxic T lymphocyte responses by a novel mouse granzyme B ELISPOT assay
Title | Evaluating antigen-specific cytotoxic T lymphocyte responses by a novel mouse granzyme B ELISPOT assay |
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Authors | |
Keywords | 51Cr-release Cytotoxicity ELISPOT Granzyme B |
Issue Date | 2006 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jim |
Citation | Journal Of Immunological Methods, 2006, v. 308 n. 1-2, p. 156-166 How to Cite? |
Abstract | We have established novel ELISA- and ELISPOT-based assays specific for the detection of a potent cytotoxic mediator, granzyme B (GrB), for the assessment of antigen-specific cytotoxic T lymphocyte responses in mice. The sensitivity and specificity of our assays was demonstrated by ELISA using purified mouse GrB and supernatants and cell lysates of cytotoxic lymphocytes derived from GrB-deficient mice. No reactivity was observed by the GrB ELISA in GrB-deficient cells. The mouse GrB ELISPOT was successfully employed to detect antigen-specific effector cell responses of two CTL clones, producing GrB ELISPOT results that correlated strongly with target cell lysis, as assessed by 51Cr-release. Furthermore, we were able to demonstrate direct correlations between GrB ELISPOT and killing by LCMV gp33-specific effector and memory T cells generated in vivo. Thus, the mouse GrB ELISPOT may be used to detect cytotoxic responses, at the single-cell level, for the functional assessment of antigen-specific CD8+ T cell responses in mouse models of infection. © 2005 Elsevier B.V. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/179429 |
ISSN | 2021 Impact Factor: 2.287 2020 SCImago Journal Rankings: 0.870 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ewen, CL | en_US |
dc.contributor.author | Rong, J | en_US |
dc.contributor.author | Kokaji, AI | en_US |
dc.contributor.author | Bleackley, RC | en_US |
dc.contributor.author | Kane, KP | en_US |
dc.date.accessioned | 2012-12-19T09:56:25Z | - |
dc.date.available | 2012-12-19T09:56:25Z | - |
dc.date.issued | 2006 | en_US |
dc.identifier.citation | Journal Of Immunological Methods, 2006, v. 308 n. 1-2, p. 156-166 | en_US |
dc.identifier.issn | 0022-1759 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179429 | - |
dc.description.abstract | We have established novel ELISA- and ELISPOT-based assays specific for the detection of a potent cytotoxic mediator, granzyme B (GrB), for the assessment of antigen-specific cytotoxic T lymphocyte responses in mice. The sensitivity and specificity of our assays was demonstrated by ELISA using purified mouse GrB and supernatants and cell lysates of cytotoxic lymphocytes derived from GrB-deficient mice. No reactivity was observed by the GrB ELISA in GrB-deficient cells. The mouse GrB ELISPOT was successfully employed to detect antigen-specific effector cell responses of two CTL clones, producing GrB ELISPOT results that correlated strongly with target cell lysis, as assessed by 51Cr-release. Furthermore, we were able to demonstrate direct correlations between GrB ELISPOT and killing by LCMV gp33-specific effector and memory T cells generated in vivo. Thus, the mouse GrB ELISPOT may be used to detect cytotoxic responses, at the single-cell level, for the functional assessment of antigen-specific CD8+ T cell responses in mouse models of infection. © 2005 Elsevier B.V. All rights reserved. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jim | en_US |
dc.relation.ispartof | Journal of Immunological Methods | en_US |
dc.subject | 51Cr-release | - |
dc.subject | Cytotoxicity | - |
dc.subject | ELISPOT | - |
dc.subject | Granzyme B | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antigens, Viral | en_US |
dc.subject.mesh | Cell Degranulation | en_US |
dc.subject.mesh | Cytotoxicity Tests, Immunologic - Methods - Statistics & Numerical Data | en_US |
dc.subject.mesh | Enzyme-Linked Immunosorbent Assay - Methods - Statistics & Numerical Data | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Granzymes | en_US |
dc.subject.mesh | Immunologic Memory | en_US |
dc.subject.mesh | Lymphocytic Choriomeningitis Virus - Immunology | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Mice, Inbred Balb C | en_US |
dc.subject.mesh | Mice, Inbred C57bl | en_US |
dc.subject.mesh | Sensitivity And Specificity | en_US |
dc.subject.mesh | Serine Endopeptidases - Analysis - Biosynthesis | en_US |
dc.subject.mesh | T-Lymphocytes, Cytotoxic - Enzymology - Immunology | en_US |
dc.title | Evaluating antigen-specific cytotoxic T lymphocyte responses by a novel mouse granzyme B ELISPOT assay | en_US |
dc.type | Article | en_US |
dc.identifier.email | Rong, J: jrong@hku.hk | en_US |
dc.identifier.authority | Rong, J=rp00515 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.jim.2005.10.009 | en_US |
dc.identifier.pmid | 16375915 | - |
dc.identifier.scopus | eid_2-s2.0-30944445218 | en_US |
dc.identifier.hkuros | 130743 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-30944445218&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 308 | en_US |
dc.identifier.issue | 1-2 | en_US |
dc.identifier.spage | 156 | en_US |
dc.identifier.epage | 166 | en_US |
dc.identifier.isi | WOS:000235285700015 | - |
dc.publisher.place | Netherlands | en_US |
dc.identifier.scopusauthorid | Ewen, CL=6603081465 | en_US |
dc.identifier.scopusauthorid | Rong, J=7005980047 | en_US |
dc.identifier.scopusauthorid | Kokaji, AI=8238711200 | en_US |
dc.identifier.scopusauthorid | Bleackley, RC=35477484000 | en_US |
dc.identifier.scopusauthorid | Kane, KP=24534271300 | en_US |
dc.identifier.issnl | 0022-1759 | - |