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Article: Protective effect of Buyanghuanwu Tang on cultured rat cortical neurons against hypoxiainduced apoptosis.

TitleProtective effect of Buyanghuanwu Tang on cultured rat cortical neurons against hypoxiainduced apoptosis.
Authors
Issue Date2002
Publisher第一軍醫大學. The Journal's web site is located at http://dyjd.chinajournal.net.cn/
Citation
Di Yi Jun Yi Da Xue Xue Bao = Academic Journal Of The First Medical College Of Pla, 2002, v. 22 n. 1, p. 35-38 How to Cite?
AbstractOBJECTIVE: To investigate the protective effect of Buyanghuanwu Tang (BYHWT), a decoction with 6 herbal ingredients, on in vitro cultured rat cortical neurons against apoptosis induced by hypoxia, and study its effect on the production of nitric oxide (NO) and oxygen free radicals and Bcl-2 expression in the neurons during hypoxia. METHODS: Models of hypoxia-induced neuron apoptosis were established and treated with sera containing BYHWT. Stained by propidium iodide, the neurons underwent apoptosis analysis using flow cytometry, and the levels of malonyl dialdehyde (MDA) and NO were measured with spectrophotometer, with Bcl-2 expression assayed by immunohistochemical method with flow cytometry. RESULTS: In BYHWT-treated neurons, apoptosis rates were significantly lower than those of the neurons subjected to hypoxia exclusively, and at the same time NO and MDA production was remarkably reduced. Bcl-2 expression, however, was up-regulated. CONCLUSION: BYHWT can protect neurons from hypoxia-induced apoptosis, the mechanism of which may lie in the elimination of NO and oxygen free radicals produced during hypoxia and up-regulation of Bcl-2 expression.
Persistent Identifierhttp://hdl.handle.net/10722/179412
ISSN

 

DC FieldValueLanguage
dc.contributor.authorQu, HDen_US
dc.contributor.authorTong, Len_US
dc.contributor.authorShen, JGen_US
dc.contributor.authorChen, YYen_US
dc.date.accessioned2012-12-19T09:56:16Z-
dc.date.available2012-12-19T09:56:16Z-
dc.date.issued2002en_US
dc.identifier.citationDi Yi Jun Yi Da Xue Xue Bao = Academic Journal Of The First Medical College Of Pla, 2002, v. 22 n. 1, p. 35-38en_US
dc.identifier.issn1000-2588en_US
dc.identifier.urihttp://hdl.handle.net/10722/179412-
dc.description.abstractOBJECTIVE: To investigate the protective effect of Buyanghuanwu Tang (BYHWT), a decoction with 6 herbal ingredients, on in vitro cultured rat cortical neurons against apoptosis induced by hypoxia, and study its effect on the production of nitric oxide (NO) and oxygen free radicals and Bcl-2 expression in the neurons during hypoxia. METHODS: Models of hypoxia-induced neuron apoptosis were established and treated with sera containing BYHWT. Stained by propidium iodide, the neurons underwent apoptosis analysis using flow cytometry, and the levels of malonyl dialdehyde (MDA) and NO were measured with spectrophotometer, with Bcl-2 expression assayed by immunohistochemical method with flow cytometry. RESULTS: In BYHWT-treated neurons, apoptosis rates were significantly lower than those of the neurons subjected to hypoxia exclusively, and at the same time NO and MDA production was remarkably reduced. Bcl-2 expression, however, was up-regulated. CONCLUSION: BYHWT can protect neurons from hypoxia-induced apoptosis, the mechanism of which may lie in the elimination of NO and oxygen free radicals produced during hypoxia and up-regulation of Bcl-2 expression.en_US
dc.languageengen_US
dc.publisher第一軍醫大學. The Journal's web site is located at http://dyjd.chinajournal.net.cn/en_US
dc.relation.ispartofDi yi jun yi da xue xue bao = Academic journal of the First Medical College of PLAen_US
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosisen_US
dc.subject.meshCell Hypoxia - Drug Effectsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCerebral Cortex - Cytologyen_US
dc.subject.meshDrugs, Chinese Herbal - Pharmacologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshMaleen_US
dc.subject.meshMedicine, Chinese Traditionalen_US
dc.subject.meshNeurons - Cytology - Drug Effectsen_US
dc.subject.meshOxygen - Metabolismen_US
dc.subject.meshProtective Agents - Pharmacologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Wistaren_US
dc.titleProtective effect of Buyanghuanwu Tang on cultured rat cortical neurons against hypoxiainduced apoptosis.en_US
dc.typeArticleen_US
dc.identifier.emailShen, JG: shenjg@hku.hken_US
dc.identifier.authorityShen, JG=rp00487en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid12390839-
dc.identifier.scopuseid_2-s2.0-0036370509en_US
dc.identifier.volume22en_US
dc.identifier.issue1en_US
dc.identifier.spage35en_US
dc.identifier.epage38en_US
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridQu, HD=7101947180en_US
dc.identifier.scopusauthoridTong, L=7201891650en_US
dc.identifier.scopusauthoridShen, JG=7404929947en_US
dc.identifier.scopusauthoridChen, YY=7601427941en_US

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