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- Publisher Website: 10.1016/S0197-0186(01)00032-8
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- PMID: 11551672
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Article: Modulation of the gene expression of N-methyl-D-aspartate receptor NR2B subunit in the rat neostriatum by a single dose of specific antisense oligodeoxynucleotide
Title | Modulation of the gene expression of N-methyl-D-aspartate receptor NR2B subunit in the rat neostriatum by a single dose of specific antisense oligodeoxynucleotide |
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Authors | |
Keywords | Basal ganglia Excitatory amino acid receptors Immunofluorescence Motor systems Pharmacology and modulation Physiology Reverse transcriptase-polymerase chain reaction |
Issue Date | 2001 |
Publisher | Elsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/neuint |
Citation | Neurochemistry International, 2001, v. 39 n. 4, p. 319-327 How to Cite? |
Abstract | N-methyl-D-aspartate receptors (NRs) are a group of ionotropic glutamate receptors in the brain and they are composed of heteromeric subunits (NR1, NR2A-D and NR3). In the neostriatum, a brain region that is associated with movement in animals, NMDA channels are known to involve in the motor control. Our previous report (Lai et al., 2000, Neuroscience 98, 493-500) has shown that a single dose of antisense oligodeoxynucleotides that are specific to NR1 subunit results in blockage of the gene expression of NR1 as well as NR2A subunits in the neostriatum. In the present study, antisense oligodeoxynucleotides that are specific to NR2B (ANR2B) were then employed as molecular tools to further investigate the molecular interactions of NMDA receptor subunits in the neostriatum. A single dose of ANR2B was injected unilaterally into the rat neostriatum. After one day of injection, no modification of motor behavior was found in the ANR2B-injected rats. The mRNA level of NR2B in the ANR2B-injected neostriatum was found to be decreased (-20.4%) by reverse transcriptase polymerase chain reaction (RT-PCR). However, the mRNA levels of NR1, NR2A, NR2C and NR2D in the ANR2B-treated neostriatum were found to be unchanged. After two days of injection, NR2B immunoreactivity was found to decrease in the ANR2B-treated neostriatum by immunofluorescence (-35.1%). At higher magnification, NR2B immunoreactivity was found to decrease in presumed spiny neurons of the neostriatum (-23.4%). No change in NR1 immunoreactivity was observed. These results indicate that a single dose of ANR2B can successfully block the gene expression of NR2B in neurons of the neostriatum and there is less effect on NR1 and other NR2 subunits. The blockage of the gene expression of NR2B is therefore specific and the present results may provide important implications in applications of antisense in research and in clinical therapy of neurological diseases. © 2001 Elsevier Science Ltd. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/179409 |
ISSN | 2023 Impact Factor: 4.4 2023 SCImago Journal Rankings: 1.049 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sze, SCW | en_US |
dc.contributor.author | Wong, CKC | en_US |
dc.contributor.author | Yung, KKL | en_US |
dc.date.accessioned | 2012-12-19T09:56:14Z | - |
dc.date.available | 2012-12-19T09:56:14Z | - |
dc.date.issued | 2001 | en_US |
dc.identifier.citation | Neurochemistry International, 2001, v. 39 n. 4, p. 319-327 | en_US |
dc.identifier.issn | 0197-0186 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179409 | - |
dc.description.abstract | N-methyl-D-aspartate receptors (NRs) are a group of ionotropic glutamate receptors in the brain and they are composed of heteromeric subunits (NR1, NR2A-D and NR3). In the neostriatum, a brain region that is associated with movement in animals, NMDA channels are known to involve in the motor control. Our previous report (Lai et al., 2000, Neuroscience 98, 493-500) has shown that a single dose of antisense oligodeoxynucleotides that are specific to NR1 subunit results in blockage of the gene expression of NR1 as well as NR2A subunits in the neostriatum. In the present study, antisense oligodeoxynucleotides that are specific to NR2B (ANR2B) were then employed as molecular tools to further investigate the molecular interactions of NMDA receptor subunits in the neostriatum. A single dose of ANR2B was injected unilaterally into the rat neostriatum. After one day of injection, no modification of motor behavior was found in the ANR2B-injected rats. The mRNA level of NR2B in the ANR2B-injected neostriatum was found to be decreased (-20.4%) by reverse transcriptase polymerase chain reaction (RT-PCR). However, the mRNA levels of NR1, NR2A, NR2C and NR2D in the ANR2B-treated neostriatum were found to be unchanged. After two days of injection, NR2B immunoreactivity was found to decrease in the ANR2B-treated neostriatum by immunofluorescence (-35.1%). At higher magnification, NR2B immunoreactivity was found to decrease in presumed spiny neurons of the neostriatum (-23.4%). No change in NR1 immunoreactivity was observed. These results indicate that a single dose of ANR2B can successfully block the gene expression of NR2B in neurons of the neostriatum and there is less effect on NR1 and other NR2 subunits. The blockage of the gene expression of NR2B is therefore specific and the present results may provide important implications in applications of antisense in research and in clinical therapy of neurological diseases. © 2001 Elsevier Science Ltd. All rights reserved. | en_US |
dc.language | eng | en_US |
dc.publisher | Elsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/neuint | en_US |
dc.relation.ispartof | Neurochemistry International | en_US |
dc.subject | Basal ganglia | - |
dc.subject | Excitatory amino acid receptors | - |
dc.subject | Immunofluorescence | - |
dc.subject | Motor systems | - |
dc.subject | Pharmacology and modulation | - |
dc.subject | Physiology | - |
dc.subject | Reverse transcriptase-polymerase chain reaction | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Apomorphine - Pharmacology | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Fluorescent Antibody Technique | en_US |
dc.subject.mesh | Gene Expression Regulation - Genetics | en_US |
dc.subject.mesh | Motor Activity - Drug Effects | en_US |
dc.subject.mesh | Neostriatum - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Oligonucleotides, Antisense - Pharmacology | en_US |
dc.subject.mesh | Rna, Messenger - Biosynthesis | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Sprague-Dawley | en_US |
dc.subject.mesh | Receptors, N-Methyl-D-Aspartate - Genetics - Metabolism | en_US |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_US |
dc.subject.mesh | Stereotyped Behavior - Drug Effects | en_US |
dc.title | Modulation of the gene expression of N-methyl-D-aspartate receptor NR2B subunit in the rat neostriatum by a single dose of specific antisense oligodeoxynucleotide | en_US |
dc.type | Article | en_US |
dc.identifier.email | Sze, SCW: stephens@hku.hk | en_US |
dc.identifier.authority | Sze, SCW=rp00514 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/S0197-0186(01)00032-8 | en_US |
dc.identifier.pmid | 11551672 | - |
dc.identifier.scopus | eid_2-s2.0-0034868516 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0034868516&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 39 | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.spage | 319 | en_US |
dc.identifier.epage | 327 | en_US |
dc.identifier.isi | WOS:000171597700007 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Sze, SCW=23482617000 | en_US |
dc.identifier.scopusauthorid | Wong, CKC=35276549400 | en_US |
dc.identifier.scopusauthorid | Yung, KKL=13605496000 | en_US |
dc.identifier.issnl | 0197-0186 | - |