File Download
There are no files associated with this item.
Supplementary
-
Citations:
- Scopus: 0
- Appears in Collections:
Article: Synergic effects of NO and oxygen free radicals in the injury of ischemia-reperfused myocardium - ESR studies on NO free radicals generated from ischemia-reperfused myocardium
Title | Synergic effects of NO and oxygen free radicals in the injury of ischemia-reperfused myocardium - ESR studies on NO free radicals generated from ischemia-reperfused myocardium |
---|---|
Authors | |
Keywords | Esr Ischemia-Reperfusion Injury Of Myocardium No O 2 |
Issue Date | 1996 |
Publisher | 中國科學院. The Journal's web site is located at http://jcxg.chinajournal.net.cn/ |
Citation | Science In China, Series C: Life Sciences, 1996, v. 39 n. 5, p. 491-500 How to Cite? |
Abstract | The ESR signal of NO bound to hemoglobin was detected during the ischemia-reperfusion of myocardium with low temperature ESR technique, and the synergic effects of NO and oxygen free radicals in the injury of the process were studied with this technique. Oxygen free radicals and NO bound to β-subunit of hemoglobin (β-NO complex) could be detected simultaneously in the ischemia-reperfused myocardium. Those signals could not be detected from the normal myocardium even in the presence of L-arginine. However, those signals could be detected and were dose-dependent with L-arginine in the ischemia-reperfused myocardiums and the signal could be suppressed with the inhibitor of NO synthetase, N G-nitro-L-arginine methylester (NAME). Measurement of the activities of lactate dehydrogenase (LDH) and creatine kinase (CK) in the coronary artery effluent of ischemia-reperfused heart showed that L-arginine at lower concentration (<1 mmol/L) could protect the heart from the ischemia-reperfusion injury but at higher concentration aggravate the injury Addition of NAME to the reperfusion solution could also protect the myocardium. Addition of xanthine (X)/xanthine oxidase (XO) or Fe 2+/H 2O 2 to the reperfusion solution increased the production of NO and oxygen free radicals and the ischemia-reperfused injury simultaneously. Addition of superoxide dismutase (SOD) and catalase decreased the production of NO and oxygen free radicals and the ischemia-reperfusion injury. |
Persistent Identifier | http://hdl.handle.net/10722/179400 |
ISSN | 2011 Impact Factor: 1.610 |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zhao, B | en_US |
dc.contributor.author | Shen, J | en_US |
dc.contributor.author | Hu, J | en_US |
dc.contributor.author | Wan, Q | en_US |
dc.contributor.author | Xin, W | en_US |
dc.date.accessioned | 2012-12-19T09:56:11Z | - |
dc.date.available | 2012-12-19T09:56:11Z | - |
dc.date.issued | 1996 | en_US |
dc.identifier.citation | Science In China, Series C: Life Sciences, 1996, v. 39 n. 5, p. 491-500 | en_US |
dc.identifier.issn | 1006-9305 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179400 | - |
dc.description.abstract | The ESR signal of NO bound to hemoglobin was detected during the ischemia-reperfusion of myocardium with low temperature ESR technique, and the synergic effects of NO and oxygen free radicals in the injury of the process were studied with this technique. Oxygen free radicals and NO bound to β-subunit of hemoglobin (β-NO complex) could be detected simultaneously in the ischemia-reperfused myocardium. Those signals could not be detected from the normal myocardium even in the presence of L-arginine. However, those signals could be detected and were dose-dependent with L-arginine in the ischemia-reperfused myocardiums and the signal could be suppressed with the inhibitor of NO synthetase, N G-nitro-L-arginine methylester (NAME). Measurement of the activities of lactate dehydrogenase (LDH) and creatine kinase (CK) in the coronary artery effluent of ischemia-reperfused heart showed that L-arginine at lower concentration (<1 mmol/L) could protect the heart from the ischemia-reperfusion injury but at higher concentration aggravate the injury Addition of NAME to the reperfusion solution could also protect the myocardium. Addition of xanthine (X)/xanthine oxidase (XO) or Fe 2+/H 2O 2 to the reperfusion solution increased the production of NO and oxygen free radicals and the ischemia-reperfused injury simultaneously. Addition of superoxide dismutase (SOD) and catalase decreased the production of NO and oxygen free radicals and the ischemia-reperfusion injury. | en_US |
dc.language | eng | en_US |
dc.publisher | 中國科學院. The Journal's web site is located at http://jcxg.chinajournal.net.cn/ | en_US |
dc.relation.ispartof | Science in China, Series C: Life Sciences | en_US |
dc.subject | Esr | en_US |
dc.subject | Ischemia-Reperfusion Injury Of Myocardium | en_US |
dc.subject | No | en_US |
dc.subject | O 2 | en_US |
dc.title | Synergic effects of NO and oxygen free radicals in the injury of ischemia-reperfused myocardium - ESR studies on NO free radicals generated from ischemia-reperfused myocardium | en_US |
dc.type | Article | en_US |
dc.identifier.email | Shen, J: shenjg@hku.hk | en_US |
dc.identifier.authority | Shen, J=rp00487 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.scopus | eid_2-s2.0-0030251771 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0030251771&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 39 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.spage | 491 | en_US |
dc.identifier.epage | 500 | en_US |
dc.publisher.place | China | en_US |
dc.identifier.scopusauthorid | Zhao, B=7403058931 | en_US |
dc.identifier.scopusauthorid | Shen, J=7404929947 | en_US |
dc.identifier.scopusauthorid | Hu, J=7406416241 | en_US |
dc.identifier.scopusauthorid | Wan, Q=8891428800 | en_US |
dc.identifier.scopusauthorid | Xin, W=7102977683 | en_US |
dc.identifier.issnl | 1006-9305 | - |