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Article: Protective effect of Phellinus linteus polysaccharide extracts against thioacetamide-induced liver fibrosis in rats: A proteomics analysis

TitleProtective effect of Phellinus linteus polysaccharide extracts against thioacetamide-induced liver fibrosis in rats: A proteomics analysis
Authors
Issue Date2012
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.cmjournal.org/home
Citation
Chinese Medicine, 2012, v. 7 n. 1, p. 23 How to Cite?
AbstractBackground: The hepatoprotective potential of Phellinus linteus polysaccharide (PLP) extracts has been described. However, the molecular mechanism of PLP for the inhibition of liver fibrosis is unclear. This study aims to investigate the molecular protein signatures involved in the hepatoprotective mechanisms of PLP via a proteomics approach using a thioacetamide (TAA)-induced liver fibrosis rat model. Methods: Male Sprague--Dawley rats were divided into three groups of six as follows: Normal group; TAA group, in which rats received TAA only; and PLP group, in which rats received PLP and TAA. Liver fibrosis was induced in the rats by repeated intraperitoneal injections of TAA at a dose of 200 mg/kg body weight twice a week for 4 weeks. PLP was given orally at a dose of 50 mg/kg body weight twice a day from the beginning of the TAA treatment until the end of the experiment. The development of liver cirrhosis was verified by histological examination. Liver proteomes were established by two-dimensional gel electrophoresis. Proteins with significantly altered expression levels were identified by matrix-assisted laser desorption/ionization-time of flight/time of flight mass spectrometry and the differentially expressed proteins were validated by immunohistochemical staining and reverse transcription polymerase chain reaction. Results: Histological staining showed a remarkable reduction in liver fibrosis in the rats with PLP treatment. A total of 13 differentially expressed proteins including actin, tubulin alpha-1C chain, preprohaptoglobin, hemopexin, galectin-5, glutathione S-transferase alpha-4 (GSTA4), branched chain keto acid dehydrogenase hterotetrameric E1 subunit alpha (BCKDHA), glutathione S-transferase mu (GSTmu); glyceraldehyde-3-phosphate dehydrogenase (GAPDH); thiosulfate sulfurtransferase (TFT); betaine-homocysteine S-methyltransferase 1 (BHMT1); quinoid dihydropteridine reductase (QDPR); ribonuclease UK114 were observed between the TAA and PLP groups. These proteins are involved in oxidative stress, heme and iron metabolism, cysteine metabolism, and branched-chain amino acid catabolism. Conclusion: The proteomics data indicate that P. linteus may be protective against TAA-induced liver fibrosis via regulation of oxidative stress pathways, heat shock pathways, and metabolic pathways for amino acids and nucleic acids.
Persistent Identifierhttp://hdl.handle.net/10722/179324
ISSN
2015 Impact Factor: 1.58
2015 SCImago Journal Rankings: 0.655
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, Hen_US
dc.contributor.authorWu, Gen_US
dc.contributor.authorPark, HJen_US
dc.contributor.authorJiang, PPen_US
dc.contributor.authorSit, WHen_US
dc.contributor.authorVan Griensven, LJLDen_US
dc.contributor.authorWan, JMFen_US
dc.date.accessioned2012-12-19T09:54:11Z-
dc.date.available2012-12-19T09:54:11Z-
dc.date.issued2012en_US
dc.identifier.citationChinese Medicine, 2012, v. 7 n. 1, p. 23en_US
dc.identifier.issn1749-8546en_US
dc.identifier.urihttp://hdl.handle.net/10722/179324-
dc.description.abstractBackground: The hepatoprotective potential of Phellinus linteus polysaccharide (PLP) extracts has been described. However, the molecular mechanism of PLP for the inhibition of liver fibrosis is unclear. This study aims to investigate the molecular protein signatures involved in the hepatoprotective mechanisms of PLP via a proteomics approach using a thioacetamide (TAA)-induced liver fibrosis rat model. Methods: Male Sprague--Dawley rats were divided into three groups of six as follows: Normal group; TAA group, in which rats received TAA only; and PLP group, in which rats received PLP and TAA. Liver fibrosis was induced in the rats by repeated intraperitoneal injections of TAA at a dose of 200 mg/kg body weight twice a week for 4 weeks. PLP was given orally at a dose of 50 mg/kg body weight twice a day from the beginning of the TAA treatment until the end of the experiment. The development of liver cirrhosis was verified by histological examination. Liver proteomes were established by two-dimensional gel electrophoresis. Proteins with significantly altered expression levels were identified by matrix-assisted laser desorption/ionization-time of flight/time of flight mass spectrometry and the differentially expressed proteins were validated by immunohistochemical staining and reverse transcription polymerase chain reaction. Results: Histological staining showed a remarkable reduction in liver fibrosis in the rats with PLP treatment. A total of 13 differentially expressed proteins including actin, tubulin alpha-1C chain, preprohaptoglobin, hemopexin, galectin-5, glutathione S-transferase alpha-4 (GSTA4), branched chain keto acid dehydrogenase hterotetrameric E1 subunit alpha (BCKDHA), glutathione S-transferase mu (GSTmu); glyceraldehyde-3-phosphate dehydrogenase (GAPDH); thiosulfate sulfurtransferase (TFT); betaine-homocysteine S-methyltransferase 1 (BHMT1); quinoid dihydropteridine reductase (QDPR); ribonuclease UK114 were observed between the TAA and PLP groups. These proteins are involved in oxidative stress, heme and iron metabolism, cysteine metabolism, and branched-chain amino acid catabolism. Conclusion: The proteomics data indicate that P. linteus may be protective against TAA-induced liver fibrosis via regulation of oxidative stress pathways, heat shock pathways, and metabolic pathways for amino acids and nucleic acids.en_US
dc.languageengen_US
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.cmjournal.org/homeen_US
dc.relation.ispartofChinese Medicineen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleProtective effect of Phellinus linteus polysaccharide extracts against thioacetamide-induced liver fibrosis in rats: A proteomics analysisen_US
dc.typeArticleen_US
dc.identifier.emailWan, JMF: jmfwan@hku.hken_US
dc.identifier.authorityWan, JMF=rp00798en_US
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1186/1749-8546-7-23en_US
dc.identifier.pmid23075396-
dc.identifier.pmcidPMC3536605-
dc.identifier.scopuseid_2-s2.0-84867519082en_US
dc.identifier.hkuros226566-
dc.identifier.volume7-
dc.identifier.issue1-
dc.identifier.spage23en_US
dc.identifier.isiWOS:000320458500001-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridWang, H=55392428600en_US
dc.identifier.scopusauthoridWu, G=55392383900en_US
dc.identifier.scopusauthoridPark, HJ=55392205700en_US
dc.identifier.scopusauthoridJiang, PP=36147603700en_US
dc.identifier.scopusauthoridSit, WH=55390946600en_US
dc.identifier.scopusauthoridvan Griensven, LJLD=55389946800en_US
dc.identifier.scopusauthoridWan, JMF=8930305000en_US

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