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Article: Gender-specific modulation of immune system complement gene expression in marine medaka Oryzias melastigma following dietary exposure of BDE-47.

TitleGender-specific modulation of immune system complement gene expression in marine medaka Oryzias melastigma following dietary exposure of BDE-47.
Authors
Issue Date2011
Citation
Environmental Science And Pollution Research International, 2011, v. 19 n. 7, p. 2477-2487 How to Cite?
AbstractBDE-47 is one of the most widely found congeners of PBDEs in marine environments. The potential immunomodulatory effects of BDE-47 on fish complement system were studied using the marine medaka Oryzias melastigma as a model fish. Three-month-old O. melastigma were subjected to short-term (5 days) and long-term (21 days) exposure to two concentrations of BDE-47 (low dose at 290 ± 172 ng/day; high dose at 580 ± 344 ng/day) via dietary uptake of BDE-47 encapsulated in Artemia nauplii. Body burdens of BDE-47 and other metabolic products were analyzed in the exposed and control fish. Only a small amount of debrominated product, BDE-28, was detected, while other metabolic products were all under detection limit. Transcriptional expression of six major complement system genes involved in complement activation: C1r/s (classical pathway), MBL-2 (lectin pathway), CFP (alternative pathway), F2 (coagulation pathway), C3 (the central component of complement system), and C9 (cell lysis) were quantified in the liver of marine medaka. Endogenous expression of all six complement system genes was found to be higher in males than in females (p < 0.05). Upon dietary exposure of marine medaka to BDE-47, expression of all six complement genes were downregulated in males at day 5 (or longer), whereas in females, MBl-2, CFP, and F2 mRNAs expression were upregulated, but C3 and C9 remained stable with exposure time and dose. A significant negative relationship was found between BDE-47 body burden and mRNA expression of C1r/s, CFP, and C3 in male fish (r = -0.8576 to -0.9447). The above findings on changes in complement gene expression patterns indicate the complement system may be compromised in male O. melastigma upon dietary exposure to BDE-47. Distinct gender difference in expression of six major complement system genes was evident in marine medaka under resting condition and dietary BDE-47 challenge. The immunomodulatory effects of BDE-47 on transcriptional expression of these complement components in marine medaka were likely induced by the parent compound instead of biotransformed products. Our results clearly demonstrate that future direction for fish immunotoxicology and risk assessment of immunosuppressive chemicals must include parallel evaluation for both genders.
Persistent Identifierhttp://hdl.handle.net/10722/179323
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYe, RRen_US
dc.contributor.authorLei, ENen_US
dc.contributor.authorLam, MHen_US
dc.contributor.authorChan, AKen_US
dc.contributor.authorBo, Jen_US
dc.contributor.authorVan De Merwe, JPen_US
dc.contributor.authorFong, ACen_US
dc.contributor.authorYang, MMen_US
dc.contributor.authorLee, JSen_US
dc.contributor.authorSegner, HEen_US
dc.contributor.authorWong, CKen_US
dc.contributor.authorWu, RSen_US
dc.contributor.authorAu, DWen_US
dc.date.accessioned2012-12-19T09:54:10Z-
dc.date.available2012-12-19T09:54:10Z-
dc.date.issued2011en_US
dc.identifier.citationEnvironmental Science And Pollution Research International, 2011, v. 19 n. 7, p. 2477-2487en_US
dc.identifier.issn1614-7499en_US
dc.identifier.urihttp://hdl.handle.net/10722/179323-
dc.description.abstractBDE-47 is one of the most widely found congeners of PBDEs in marine environments. The potential immunomodulatory effects of BDE-47 on fish complement system were studied using the marine medaka Oryzias melastigma as a model fish. Three-month-old O. melastigma were subjected to short-term (5 days) and long-term (21 days) exposure to two concentrations of BDE-47 (low dose at 290 ± 172 ng/day; high dose at 580 ± 344 ng/day) via dietary uptake of BDE-47 encapsulated in Artemia nauplii. Body burdens of BDE-47 and other metabolic products were analyzed in the exposed and control fish. Only a small amount of debrominated product, BDE-28, was detected, while other metabolic products were all under detection limit. Transcriptional expression of six major complement system genes involved in complement activation: C1r/s (classical pathway), MBL-2 (lectin pathway), CFP (alternative pathway), F2 (coagulation pathway), C3 (the central component of complement system), and C9 (cell lysis) were quantified in the liver of marine medaka. Endogenous expression of all six complement system genes was found to be higher in males than in females (p < 0.05). Upon dietary exposure of marine medaka to BDE-47, expression of all six complement genes were downregulated in males at day 5 (or longer), whereas in females, MBl-2, CFP, and F2 mRNAs expression were upregulated, but C3 and C9 remained stable with exposure time and dose. A significant negative relationship was found between BDE-47 body burden and mRNA expression of C1r/s, CFP, and C3 in male fish (r = -0.8576 to -0.9447). The above findings on changes in complement gene expression patterns indicate the complement system may be compromised in male O. melastigma upon dietary exposure to BDE-47. Distinct gender difference in expression of six major complement system genes was evident in marine medaka under resting condition and dietary BDE-47 challenge. The immunomodulatory effects of BDE-47 on transcriptional expression of these complement components in marine medaka were likely induced by the parent compound instead of biotransformed products. Our results clearly demonstrate that future direction for fish immunotoxicology and risk assessment of immunosuppressive chemicals must include parallel evaluation for both genders.en_US
dc.languageengen_US
dc.relation.ispartofEnvironmental science and pollution research internationalen_US
dc.subject.meshAdministration, Oralen_US
dc.subject.meshAnimal Feeden_US
dc.subject.meshAnimalsen_US
dc.subject.meshArtemiaen_US
dc.subject.meshComplement System Proteins - Genetics - Metabolismen_US
dc.subject.meshDieten_US
dc.subject.meshFemaleen_US
dc.subject.meshFish Proteins - Genetics - Metabolismen_US
dc.subject.meshGene Expression Regulation - Immunologyen_US
dc.subject.meshMaleen_US
dc.subject.meshOryziasen_US
dc.subject.meshPolybrominated Biphenyls - Administration & Dosage - Toxicityen_US
dc.subject.meshRna, Messenger - Genetics - Metabolismen_US
dc.subject.meshSex Factorsen_US
dc.subject.meshWater Pollutants, Chemical - Administration & Dosage - Toxicityen_US
dc.titleGender-specific modulation of immune system complement gene expression in marine medaka Oryzias melastigma following dietary exposure of BDE-47.en_US
dc.typeArticleen_US
dc.identifier.emailWu, RS: rudolfwu@hku.hken_US
dc.identifier.authorityWu, RS=rp01398en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s11356-012-0887-zen_US
dc.identifier.pmid22828878-
dc.identifier.scopuseid_2-s2.0-84867481986en_US
dc.identifier.volume19en_US
dc.identifier.issue7en_US
dc.identifier.spage2477en_US
dc.identifier.epage2487en_US
dc.identifier.isiWOS:000306790200003-
dc.identifier.scopusauthoridYe, RR=55389042100en_US
dc.identifier.scopusauthoridLei, EN=36465365000en_US
dc.identifier.scopusauthoridLam, MH=7202630175en_US
dc.identifier.scopusauthoridChan, AK=24278666900en_US
dc.identifier.scopusauthoridBo, J=55389621300en_US
dc.identifier.scopusauthoridvan de Merwe, JP=8701906400en_US
dc.identifier.scopusauthoridFong, AC=55389444300en_US
dc.identifier.scopusauthoridYang, MM=55389097600en_US
dc.identifier.scopusauthoridLee, JS=55389852000en_US
dc.identifier.scopusauthoridSegner, HE=7004956984en_US
dc.identifier.scopusauthoridWong, CK=35276549400en_US
dc.identifier.scopusauthoridWu, RS=7402945079en_US
dc.identifier.scopusauthoridAu, DW=7004909228en_US

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