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Article: Analysis of alkane-dependent methanogenic community derived from production water of a high-temperature petroleum reservoir

TitleAnalysis of alkane-dependent methanogenic community derived from production water of a high-temperature petroleum reservoir
Authors
KeywordsAlkylsuccinate Synthase
High-Temperature Petroleum Reservoir
Hydrogenotrophic Methanogenesis
Methanogenic Degradation Of Alkanes
Microbial Community
Syntrophic Acetate Oxidation
Issue Date2012
PublisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm
Citation
Applied Microbiology And Biotechnology, 2012, v. 96 n. 2, p. 531-542 How to Cite?
AbstractMicrobial assemblage in an n-alkanes-dependent thermophilic methanogenic enrichment cultures derived from production waters of a high-temperature petroleum reservoir was investigated in this study. Substantially higher amounts of methane were generated from the enrichment cultures incubated at 55 °C for 528 days with a mixture of long-chain n-alkanes (C 15-C 20). Stoichiometric estimation showed that alkanes-dependent methanogenesis accounted for about 19.8% of the total amount of methane expected. Hydrogen was occasionally detected together with methane in the gas phase of the cultures. Chemical analysis of the liquid cultures resulted only in low concentrations of acetate and formate. Phylogenetic analysis of the enrichment revealed the presence of several bacterial taxa related to Firmicutes, Thermodesulfobiaceae, Thermotogaceae, Nitrospiraceae, Dictyoglomaceae, Candidate division OP8 and others without close cultured representatives, and Archaea predominantly related to uncultured members in the order Archaeoglobales and CO 2-reducing methanogens. Screening of genomic DNA retrieved from the alkanesamended enrichment cultures also suggested the presence of new alkylsuccinate synthase alpha-subunit (assA) homologues. These findings suggest the presence of poorly characterized (putative) anaerobic n-alkanes degraders in the thermophilic methanogenic enrichment cultures. Our results indicate that methanogenesis of alkanes under thermophilic condition is likely to proceed via syntrophic acetate and/or formate oxidation linked with hydrogenotrophic methanogenesis. © Springer-Verlag 2012.
Persistent Identifierhttp://hdl.handle.net/10722/179312
ISSN
2015 Impact Factor: 3.376
2015 SCImago Journal Rankings: 1.262
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMbadinga, SMen_US
dc.contributor.authorLi, KPen_US
dc.contributor.authorZhou, Len_US
dc.contributor.authorWang, LYen_US
dc.contributor.authorYang, SZen_US
dc.contributor.authorLiu, JFen_US
dc.contributor.authorGu, JDen_US
dc.contributor.authorMu, BZen_US
dc.date.accessioned2012-12-19T09:54:04Z-
dc.date.available2012-12-19T09:54:04Z-
dc.date.issued2012en_US
dc.identifier.citationApplied Microbiology And Biotechnology, 2012, v. 96 n. 2, p. 531-542en_US
dc.identifier.issn0175-7598en_US
dc.identifier.urihttp://hdl.handle.net/10722/179312-
dc.description.abstractMicrobial assemblage in an n-alkanes-dependent thermophilic methanogenic enrichment cultures derived from production waters of a high-temperature petroleum reservoir was investigated in this study. Substantially higher amounts of methane were generated from the enrichment cultures incubated at 55 °C for 528 days with a mixture of long-chain n-alkanes (C 15-C 20). Stoichiometric estimation showed that alkanes-dependent methanogenesis accounted for about 19.8% of the total amount of methane expected. Hydrogen was occasionally detected together with methane in the gas phase of the cultures. Chemical analysis of the liquid cultures resulted only in low concentrations of acetate and formate. Phylogenetic analysis of the enrichment revealed the presence of several bacterial taxa related to Firmicutes, Thermodesulfobiaceae, Thermotogaceae, Nitrospiraceae, Dictyoglomaceae, Candidate division OP8 and others without close cultured representatives, and Archaea predominantly related to uncultured members in the order Archaeoglobales and CO 2-reducing methanogens. Screening of genomic DNA retrieved from the alkanesamended enrichment cultures also suggested the presence of new alkylsuccinate synthase alpha-subunit (assA) homologues. These findings suggest the presence of poorly characterized (putative) anaerobic n-alkanes degraders in the thermophilic methanogenic enrichment cultures. Our results indicate that methanogenesis of alkanes under thermophilic condition is likely to proceed via syntrophic acetate and/or formate oxidation linked with hydrogenotrophic methanogenesis. © Springer-Verlag 2012.en_US
dc.languageengen_US
dc.publisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htmen_US
dc.relation.ispartofApplied Microbiology and Biotechnologyen_US
dc.rightsThe original publication is available at www.springerlink.com-
dc.subjectAlkylsuccinate Synthaseen_US
dc.subjectHigh-Temperature Petroleum Reservoiren_US
dc.subjectHydrogenotrophic Methanogenesisen_US
dc.subjectMethanogenic Degradation Of Alkanesen_US
dc.subjectMicrobial Communityen_US
dc.subjectSyntrophic Acetate Oxidationen_US
dc.titleAnalysis of alkane-dependent methanogenic community derived from production water of a high-temperature petroleum reservoiren_US
dc.typeArticleen_US
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_US
dc.identifier.authorityGu, JD=rp00701en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s00253-011-3828-8en_US
dc.identifier.pmid22249716-
dc.identifier.scopuseid_2-s2.0-84866516898en_US
dc.identifier.hkuros224971-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84866516898&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume96en_US
dc.identifier.issue2en_US
dc.identifier.spage531en_US
dc.identifier.epage542en_US
dc.identifier.isiWOS:000308953100023-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridMbadinga, SM=36802004800en_US
dc.identifier.scopusauthoridLi, KP=7404988931en_US
dc.identifier.scopusauthoridZhou, L=7404126715en_US
dc.identifier.scopusauthoridWang, LY=36803082300en_US
dc.identifier.scopusauthoridYang, SZ=49764409700en_US
dc.identifier.scopusauthoridLiu, JF=36096880500en_US
dc.identifier.scopusauthoridGu, JD=7403129601en_US
dc.identifier.scopusauthoridMu, BZ=7004585949en_US
dc.identifier.citeulike10248818-

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