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Article: Leptin-mediated modulation of steroidogenic gene expression in hypoxic zebrafish embryos: Implications for the disruption of sex steroids

TitleLeptin-mediated modulation of steroidogenic gene expression in hypoxic zebrafish embryos: Implications for the disruption of sex steroids
Authors
Issue Date2012
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/est
Citation
Environmental Science And Technology, 2012, v. 46 n. 16, p. 9112-9119 How to Cite?
AbstractHypoxia can impair reproduction of fishes through the disruption of sex steroids. Here, using zebrafish (Danio rerio) embryos, we investigated (i) whether hypoxia can directly affect steroidogenesis independent of pituitary regulation via modulation of steroidogenic gene expression, and (ii) the role of leptin in hypoxia-induced disruption of steroidogenesis. Exposure of fertilized zebrafish embryos to hypoxia (1.0 mg O 2 L -1) from 0-72 h postfertilization (hpf), a developmental window when steroidogenesis is unregulated by pituitary influence, resulted in the up-regulation of cyp11a, cyp17, and 3β-hsd and the down-regulation of cyp19a. Similar gene expression patterns were observed for embryos exposed to 10 mM cobalt chloride (CoCl 2, a chemical inducer of hypoxia-inducible factor 1, HIF-1), suggesting a regulatory role of HIF-1 in steroidogenesis. Testosterone (T) and estradiol (E2) concentrations in hypoxic embryos were greater and lesser, respectively, relative to the normoxic control, thus leading to an increased T/E2 ratio. Expression of the leptin-a gene (zlep-a) was up-regulated upon both hypoxia and CoCl 2 treatments. Functional assays suggested that under hypoxia, elevated zlep-a expression might activate cyp11a and 3β-hsd and inhibit cyp19a. Overall, this study indicates that hypoxia, possibly via HIF-1-induced leptin expression, modulates sex steroid synthesis by acting directly on steroidogenic gene expression. © 2012 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/179301
ISSN
2015 Impact Factor: 5.393
2015 SCImago Journal Rankings: 2.664
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYu, RMKen_US
dc.contributor.authorChu, DLHen_US
dc.contributor.authorTan, TFen_US
dc.contributor.authorLi, VWTen_US
dc.contributor.authorChan, AKYen_US
dc.contributor.authorGiesy, JPen_US
dc.contributor.authorCheng, SHen_US
dc.contributor.authorWu, RSSen_US
dc.contributor.authorKong, RYCen_US
dc.date.accessioned2012-12-19T09:53:58Z-
dc.date.available2012-12-19T09:53:58Z-
dc.date.issued2012en_US
dc.identifier.citationEnvironmental Science And Technology, 2012, v. 46 n. 16, p. 9112-9119en_US
dc.identifier.issn0013-936Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/179301-
dc.description.abstractHypoxia can impair reproduction of fishes through the disruption of sex steroids. Here, using zebrafish (Danio rerio) embryos, we investigated (i) whether hypoxia can directly affect steroidogenesis independent of pituitary regulation via modulation of steroidogenic gene expression, and (ii) the role of leptin in hypoxia-induced disruption of steroidogenesis. Exposure of fertilized zebrafish embryos to hypoxia (1.0 mg O 2 L -1) from 0-72 h postfertilization (hpf), a developmental window when steroidogenesis is unregulated by pituitary influence, resulted in the up-regulation of cyp11a, cyp17, and 3β-hsd and the down-regulation of cyp19a. Similar gene expression patterns were observed for embryos exposed to 10 mM cobalt chloride (CoCl 2, a chemical inducer of hypoxia-inducible factor 1, HIF-1), suggesting a regulatory role of HIF-1 in steroidogenesis. Testosterone (T) and estradiol (E2) concentrations in hypoxic embryos were greater and lesser, respectively, relative to the normoxic control, thus leading to an increased T/E2 ratio. Expression of the leptin-a gene (zlep-a) was up-regulated upon both hypoxia and CoCl 2 treatments. Functional assays suggested that under hypoxia, elevated zlep-a expression might activate cyp11a and 3β-hsd and inhibit cyp19a. Overall, this study indicates that hypoxia, possibly via HIF-1-induced leptin expression, modulates sex steroid synthesis by acting directly on steroidogenic gene expression. © 2012 American Chemical Society.en_US
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/esten_US
dc.relation.ispartofEnvironmental Science and Technologyen_US
dc.titleLeptin-mediated modulation of steroidogenic gene expression in hypoxic zebrafish embryos: Implications for the disruption of sex steroidsen_US
dc.typeArticleen_US
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_US
dc.identifier.authorityWu, RSS=rp01398en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/es301758cen_US
dc.identifier.pmid22816610-
dc.identifier.scopuseid_2-s2.0-84865467440en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84865467440&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume46en_US
dc.identifier.issue16en_US
dc.identifier.spage9112en_US
dc.identifier.epage9119en_US
dc.identifier.isiWOS:000307697700071-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYu, RMK=9278574900en_US
dc.identifier.scopusauthoridChu, DLH=25229992700en_US
dc.identifier.scopusauthoridTan, TF=25230516600en_US
dc.identifier.scopusauthoridLi, VWT=24069083500en_US
dc.identifier.scopusauthoridChan, AKY=24278666900en_US
dc.identifier.scopusauthoridGiesy, JP=35459135300en_US
dc.identifier.scopusauthoridCheng, SH=20233852300en_US
dc.identifier.scopusauthoridWu, RSS=7402945079en_US
dc.identifier.scopusauthoridKong, RYC=7005290687en_US

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