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Article: Diversity and distribution of sulfate-reducing bacteria in four petroleum reservoirs detected by using 16S rRNA and dsrAB genes

TitleDiversity and distribution of sulfate-reducing bacteria in four petroleum reservoirs detected by using 16S rRNA and dsrAB genes
Authors
Keywords16S rRNA Gene
Cca
Corrosion
Dsrab Genes
Petroleum Microbiology
Sulfate-Reducing Bacteria
Issue Date2013
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ibiod
Citation
International Biodeterioration and Biodegradation, 2013, v. 76, p. 58-66 How to Cite?
AbstractMicrobial sulfate reduction, an important metabolic process in petroleum reservoirs, is widely known as a major contributor to microbial influenced corrosion and deterioration of oil quality. To better control oil field corrosion and oil degradation caused by the sulfate-reducing bacteria (SRBs), the community structure and composition of SRBs in four oil reservoirs were investigated in this study by comparing clone libraries of 16S rRNA and dissimilatory sulfate reductase (dsrAB) genes. In addition, canonical correspondence analysis (CCA) was also employed to find relationship between biodata and physiochemical information. More information on SRB communities was obtained from nested-PCR-phylogenetic analyses of 16S rRNA genes and PCR primer sets amplifying six groups of SRBs frequently detected in oilfields all over the world were used. Amplified sequences belonging to Desulfotomaculum and Desulfobacter were the most dominant in all four reservoirs. The diversity of SRB communities increased while the temperature of the four oil reservoirs decreased from 63 to 21. Correlations between environmental variables and species distribution indicated that Desulfotomaculum was correlated with temperature, depth, and the concentration of acetate, propionate and sulphate. Desulfomicrobium, Desulfobacter and Desulfobulbus showed positive correlation with sulphur and salinity. Desulfobacterium was influenced by both salinity and the concentration of acetate. The results of this study provided important information on the microbial ecology of sulfate-reducing bacteria in different petroleum reservoirs. © 2012 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/179286
ISSN
2015 Impact Factor: 2.429
2015 SCImago Journal Rankings: 0.919
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGuan, Jen_US
dc.contributor.authorXia, LPen_US
dc.contributor.authorWang, LYen_US
dc.contributor.authorLiu, JFen_US
dc.contributor.authorGu, JDen_US
dc.contributor.authorMu, BZen_US
dc.date.accessioned2012-12-19T09:53:49Z-
dc.date.available2012-12-19T09:53:49Z-
dc.date.issued2013en_US
dc.identifier.citationInternational Biodeterioration and Biodegradation, 2013, v. 76, p. 58-66en_US
dc.identifier.issn0964-8305en_US
dc.identifier.urihttp://hdl.handle.net/10722/179286-
dc.description.abstractMicrobial sulfate reduction, an important metabolic process in petroleum reservoirs, is widely known as a major contributor to microbial influenced corrosion and deterioration of oil quality. To better control oil field corrosion and oil degradation caused by the sulfate-reducing bacteria (SRBs), the community structure and composition of SRBs in four oil reservoirs were investigated in this study by comparing clone libraries of 16S rRNA and dissimilatory sulfate reductase (dsrAB) genes. In addition, canonical correspondence analysis (CCA) was also employed to find relationship between biodata and physiochemical information. More information on SRB communities was obtained from nested-PCR-phylogenetic analyses of 16S rRNA genes and PCR primer sets amplifying six groups of SRBs frequently detected in oilfields all over the world were used. Amplified sequences belonging to Desulfotomaculum and Desulfobacter were the most dominant in all four reservoirs. The diversity of SRB communities increased while the temperature of the four oil reservoirs decreased from 63 to 21. Correlations between environmental variables and species distribution indicated that Desulfotomaculum was correlated with temperature, depth, and the concentration of acetate, propionate and sulphate. Desulfomicrobium, Desulfobacter and Desulfobulbus showed positive correlation with sulphur and salinity. Desulfobacterium was influenced by both salinity and the concentration of acetate. The results of this study provided important information on the microbial ecology of sulfate-reducing bacteria in different petroleum reservoirs. © 2012 Elsevier Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ibioden_US
dc.relation.ispartofInternational Biodeterioration and Biodegradationen_US
dc.subject16S rRNA Geneen_US
dc.subjectCcaen_US
dc.subjectCorrosionen_US
dc.subjectDsrab Genesen_US
dc.subjectPetroleum Microbiologyen_US
dc.subjectSulfate-Reducing Bacteriaen_US
dc.titleDiversity and distribution of sulfate-reducing bacteria in four petroleum reservoirs detected by using 16S rRNA and dsrAB genesen_US
dc.typeArticleen_US
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_US
dc.identifier.authorityGu, JD=rp00701en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.ibiod.2012.06.021en_US
dc.identifier.scopuseid_2-s2.0-84871805455en_US
dc.identifier.hkuros224968-
dc.identifier.isiWOS:000312759300011-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridGuan, J=55273841200en_US
dc.identifier.scopusauthoridXia, LP=55274556800en_US
dc.identifier.scopusauthoridWang, LY=36803082300en_US
dc.identifier.scopusauthoridLiu, JF=36096880500en_US
dc.identifier.scopusauthoridGu, JD=7403129601en_US
dc.identifier.scopusauthoridMu, BZ=7004585949en_US
dc.identifier.citeulike10865107-

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