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Article: Molecular characterization of prostaglandin F receptor (FP) and E receptor subtype 1 (EP 1) in zebrafish

TitleMolecular characterization of prostaglandin F receptor (FP) and E receptor subtype 1 (EP 1) in zebrafish
Authors
KeywordsCharacterization
Cloning
Ep 1
Fp
Prostaglandin Receptors
Zebrafish
Issue Date2012
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcen
Citation
General And Comparative Endocrinology, 2012, v. 178 n. 2, p. 216-226 How to Cite?
AbstractProstaglandins E (PGE) and F (PGF) mediate diverse physiological functions via their cell surface receptors - prostaglandin E receptor (EP) subtypes 1, 2, 3 and 4 (EP 1; EP 2; EP 3; EP 4) and F receptor (FP). In teleost fishes, PGE was implicated in gill epithelium ion transport, while both PGE and PGF were involved in oocyte maturation, follicular rupture and coordination of reproductive behaviors. However, little is known about the mechanisms behind their actions. In present study, we first identified the full-length ORF cDNA clones of three zebrafish prostaglandin E receptor subtype 1 (zEP 1) isoforms - zEP 1a, zEP 1b and zEP 1c - and FP (zFP) from adult ovary. RT-PCR showed that zEP 1a, zEP 1b and zFP are widely expressed in adult tissues, while zEP 1c mRNA expression is mainly confined in brain and kidney. Using a pGL3-NFAT-RE luciferase reporter system, both zEP 1a and zEP 1b expressed in DF-1 cells were shown to be activated by PGE 2 potently while zEP 1c and zFP were activated by PGF 2a effectively, suggesting that the four receptors are functionally coupled to intracellular Ca 2+-signaling pathway. Furthermore, EP1a and EP1b, but not EP1c were suggested to couple to cAMP-PKA signaling pathway using a pGL3-CRE luciferase reporter assay. Although zEP 1c might originate as a paralog to zEP 1a and zEP 1b, its functional coupling to PGF 2α instead of PGE 2 suggested that zEP 1 isoforms might have sub-functionalized in their ligand binding and G protein coupling specificity, in addition to differential tissue distribution. Characterization of these receptors undoubtedly furthered our understanding on the diverse yet highly target-specific responses of prostaglandins in teleosts. © 2012 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/179285
ISSN
2015 Impact Factor: 2.667
2015 SCImago Journal Rankings: 1.245
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKwok, AHYen_US
dc.contributor.authorWang, Yen_US
dc.contributor.authorLeung, FCen_US
dc.date.accessioned2012-12-19T09:53:48Z-
dc.date.available2012-12-19T09:53:48Z-
dc.date.issued2012en_US
dc.identifier.citationGeneral And Comparative Endocrinology, 2012, v. 178 n. 2, p. 216-226en_US
dc.identifier.issn0016-6480en_US
dc.identifier.urihttp://hdl.handle.net/10722/179285-
dc.description.abstractProstaglandins E (PGE) and F (PGF) mediate diverse physiological functions via their cell surface receptors - prostaglandin E receptor (EP) subtypes 1, 2, 3 and 4 (EP 1; EP 2; EP 3; EP 4) and F receptor (FP). In teleost fishes, PGE was implicated in gill epithelium ion transport, while both PGE and PGF were involved in oocyte maturation, follicular rupture and coordination of reproductive behaviors. However, little is known about the mechanisms behind their actions. In present study, we first identified the full-length ORF cDNA clones of three zebrafish prostaglandin E receptor subtype 1 (zEP 1) isoforms - zEP 1a, zEP 1b and zEP 1c - and FP (zFP) from adult ovary. RT-PCR showed that zEP 1a, zEP 1b and zFP are widely expressed in adult tissues, while zEP 1c mRNA expression is mainly confined in brain and kidney. Using a pGL3-NFAT-RE luciferase reporter system, both zEP 1a and zEP 1b expressed in DF-1 cells were shown to be activated by PGE 2 potently while zEP 1c and zFP were activated by PGF 2a effectively, suggesting that the four receptors are functionally coupled to intracellular Ca 2+-signaling pathway. Furthermore, EP1a and EP1b, but not EP1c were suggested to couple to cAMP-PKA signaling pathway using a pGL3-CRE luciferase reporter assay. Although zEP 1c might originate as a paralog to zEP 1a and zEP 1b, its functional coupling to PGF 2α instead of PGE 2 suggested that zEP 1 isoforms might have sub-functionalized in their ligand binding and G protein coupling specificity, in addition to differential tissue distribution. Characterization of these receptors undoubtedly furthered our understanding on the diverse yet highly target-specific responses of prostaglandins in teleosts. © 2012 Elsevier Inc.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcenen_US
dc.relation.ispartofGeneral and Comparative Endocrinologyen_US
dc.subjectCharacterizationen_US
dc.subjectCloningen_US
dc.subjectEp 1en_US
dc.subjectFpen_US
dc.subjectProstaglandin Receptorsen_US
dc.subjectZebrafishen_US
dc.titleMolecular characterization of prostaglandin F receptor (FP) and E receptor subtype 1 (EP 1) in zebrafishen_US
dc.typeArticleen_US
dc.identifier.emailWang, Y: cdwyj@yahoo.comen_US
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_US
dc.identifier.authorityWang, Y=rp00801en_US
dc.identifier.authorityLeung, FC=rp00731en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.ygcen.2012.05.002en_US
dc.identifier.pmid22617193-
dc.identifier.scopuseid_2-s2.0-84863190519en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84863190519&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume178en_US
dc.identifier.issue2en_US
dc.identifier.spage216en_US
dc.identifier.epage226en_US
dc.identifier.isiWOS:000307694100006-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKwok, AHY=27168105100en_US
dc.identifier.scopusauthoridWang, Y=36062525200en_US
dc.identifier.scopusauthoridLeung, FC=7103078633en_US
dc.identifier.citeulike10695260-

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