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Article: Spermatogonial stem cells alone are not sufficient to re-initiate spermatogenesis in the rat testis following adjudin-induced infertility

TitleSpermatogonial stem cells alone are not sufficient to re-initiate spermatogenesis in the rat testis following adjudin-induced infertility
Authors
Issue Date2012
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IJA
Citation
International Journal Of Andrology, 2012, v. 35 n. 1, p. 86-101 How to Cite?
AbstractThe blood-testis barrier (BTB) is a unique ultrastructure in the testis, which creates a specialized microenvironment in the seminiferous epithelium known as the apical (or adluminal) compartment for post-meiotic germ-cell development and for maintenance of an immunological barrier. In this study, we have demonstrated unequivocally that a functional and intact BTB is crucial for the initiation of spermatogenesis, in particular, the differentiation of spermatogonial stem cells (SSCs). It was shown that adult rats (~300g body weight, b.w.) treated with adjudin at 50 (low-dose) or 250 (high-dose)mg/kg b.w. by gavage led to germ-cell depletion from the seminiferous tubules and that >98% of the tubules were devoid of germ cells by ~2week and rats became infertile in both groups after the sperm reserve in the epididymis was exhausted. While the population of SSC/spermatogonia in the seminiferous tubules from both groups was similar to that of normal rats, only rats from the low-dose group were capable of re-initiating spermatogenesis; and by 20 weeks, greater than 75% of the tubules displayed normal spermatogenesis and the fertility of these rats rebounded. Detailed analysis by dual-labelled immunofluorescence analysis and a functional BTB integrity assay revealed that in both treatment groups, the BTB was disrupted from week 6 to week 12. However, the disrupted BTB 'resealed' in the low-dose group, but not in the high-dose group. Our findings illustrate that SSC/spermatogonia failed to differentiate into spermatocytes beyond A aligned spermatogonia in the high-dose group with a disrupted BTB. In short, these findings illustrate the critical significance of the BTB for re-initiation of spermatogenesis besides SSC and spermatogonia. © 2011 The Authors. International Journal of Andrology © 2011 European Academy of Andrology.
Persistent Identifierhttp://hdl.handle.net/10722/179267
ISSN
2014 Impact Factor: 3.695
2015 SCImago Journal Rankings: 1.161
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMok, KWen_US
dc.contributor.authorMruk, DDen_US
dc.contributor.authorLee, WMen_US
dc.contributor.authorCheng, CYen_US
dc.date.accessioned2012-12-19T09:53:31Z-
dc.date.available2012-12-19T09:53:31Z-
dc.date.issued2012en_US
dc.identifier.citationInternational Journal Of Andrology, 2012, v. 35 n. 1, p. 86-101en_US
dc.identifier.issn0105-6263en_US
dc.identifier.urihttp://hdl.handle.net/10722/179267-
dc.description.abstractThe blood-testis barrier (BTB) is a unique ultrastructure in the testis, which creates a specialized microenvironment in the seminiferous epithelium known as the apical (or adluminal) compartment for post-meiotic germ-cell development and for maintenance of an immunological barrier. In this study, we have demonstrated unequivocally that a functional and intact BTB is crucial for the initiation of spermatogenesis, in particular, the differentiation of spermatogonial stem cells (SSCs). It was shown that adult rats (~300g body weight, b.w.) treated with adjudin at 50 (low-dose) or 250 (high-dose)mg/kg b.w. by gavage led to germ-cell depletion from the seminiferous tubules and that >98% of the tubules were devoid of germ cells by ~2week and rats became infertile in both groups after the sperm reserve in the epididymis was exhausted. While the population of SSC/spermatogonia in the seminiferous tubules from both groups was similar to that of normal rats, only rats from the low-dose group were capable of re-initiating spermatogenesis; and by 20 weeks, greater than 75% of the tubules displayed normal spermatogenesis and the fertility of these rats rebounded. Detailed analysis by dual-labelled immunofluorescence analysis and a functional BTB integrity assay revealed that in both treatment groups, the BTB was disrupted from week 6 to week 12. However, the disrupted BTB 'resealed' in the low-dose group, but not in the high-dose group. Our findings illustrate that SSC/spermatogonia failed to differentiate into spermatocytes beyond A aligned spermatogonia in the high-dose group with a disrupted BTB. In short, these findings illustrate the critical significance of the BTB for re-initiation of spermatogenesis besides SSC and spermatogonia. © 2011 The Authors. International Journal of Andrology © 2011 European Academy of Andrology.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IJAen_US
dc.relation.ispartofInternational Journal of Andrologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshHydrazines - Metabolismen_US
dc.subject.meshIndazoles - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshRatsen_US
dc.subject.meshSpermatogenesisen_US
dc.subject.meshSpermatogonia - Pathologyen_US
dc.subject.meshStem Cells - Pathologyen_US
dc.titleSpermatogonial stem cells alone are not sufficient to re-initiate spermatogenesis in the rat testis following adjudin-induced infertilityen_US
dc.typeArticleen_US
dc.identifier.emailLee, WM: hrszlwm@hku.hken_US
dc.identifier.authorityLee, WM=rp00728en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1365-2605.2011.01183.xen_US
dc.identifier.pmid21696392-
dc.identifier.scopuseid_2-s2.0-84855669276en_US
dc.identifier.hkuros200050-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84855669276&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume35en_US
dc.identifier.issue1en_US
dc.identifier.spage86en_US
dc.identifier.epage101en_US
dc.identifier.isiWOS:000298914100011-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridMok, KW=38961643800en_US
dc.identifier.scopusauthoridMruk, DD=6701823934en_US
dc.identifier.scopusauthoridLee, WM=24799156600en_US
dc.identifier.scopusauthoridCheng, CY=7404797787en_US

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