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Article: Comparison of initial hydrolysis of the three dimethyl phthalate esters (DMPEs) by a basidiomycetous yeast, Trichosporon DMI-5-1, from coastal sediment

TitleComparison of initial hydrolysis of the three dimethyl phthalate esters (DMPEs) by a basidiomycetous yeast, Trichosporon DMI-5-1, from coastal sediment
Authors
Issue Date2011
PublisherSpringer. The Journal's web site is located at http://www.springer.com/environment/journal/11356
Citation
Environmental Science And Pollution Research, 2011, v. 18 n. 9, p. 1653-1660 How to Cite?
AbstractPurpose: Dimethyl phthalate esters (DMPEs) are a group of plasticizers commonly detected in the environment with potential adverse human health impact. The degradation of DMPEs by fungal systems has been studied to a limited extent, particularly by yeasts. In this study, a basidiomycetous yeast Trichosporon DMI-5-1 capable of degrading DMPEs was obtained and the degradation pathways were investigated. Results: A DMPE-degrading yeast was isolated from costal sediment by enrichment culture technique and was identified as Trichosporon sp. DMI-5-1 based on microscopic morphology and 18S rDNA sequence. Comparative investigations on biodegradation of three isomers of DMPEs, namely dimethyl phthalate (DMP), dimethyl isophthalate (DMI), and dimethyl terephthalate (DMT), were carried out with this yeast strain. Trichosporon sp. DMI-5-1 could not mineralize DMPEs completely but transform them to respective monomethyl phthalate or phthalic acid. Biochemical degradation pathways for the three DMPE isomers by Trichosporon sp. DMI-5-1 were apparently different. The yeast carried out one-step ester hydrolysis of DMP and DMI to respective monoesters (monomethyl phthalate and monomethyl isophthalate, respectively) and no further metabolism of these two monoesters. Meanwhile, DMT was transformed by the yeast to monomethyl terephthalate and subsequently to terephthalic acid by stepwise hydrolysis of the two ester bonds. Conclusions: This study shows that different catalytic processes are involved in the transformation of DMPEs by the basidiomycetous yeast Trichosporon sp. DMI-5-1 and suggests that its esterases, responsible for the initial hydrolyzing the two ester bonds of DMPEs, are highly substrate specific. © 2011 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/179256
ISSN
2015 Impact Factor: 2.76
2015 SCImago Journal Rankings: 0.886
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLuo, ZHen_US
dc.contributor.authorWu, YRen_US
dc.contributor.authorPang, KLen_US
dc.contributor.authorGu, JDen_US
dc.contributor.authorVrijmoed, LLPen_US
dc.date.accessioned2012-12-19T09:53:25Z-
dc.date.available2012-12-19T09:53:25Z-
dc.date.issued2011en_US
dc.identifier.citationEnvironmental Science And Pollution Research, 2011, v. 18 n. 9, p. 1653-1660en_US
dc.identifier.issn0944-1344en_US
dc.identifier.urihttp://hdl.handle.net/10722/179256-
dc.description.abstractPurpose: Dimethyl phthalate esters (DMPEs) are a group of plasticizers commonly detected in the environment with potential adverse human health impact. The degradation of DMPEs by fungal systems has been studied to a limited extent, particularly by yeasts. In this study, a basidiomycetous yeast Trichosporon DMI-5-1 capable of degrading DMPEs was obtained and the degradation pathways were investigated. Results: A DMPE-degrading yeast was isolated from costal sediment by enrichment culture technique and was identified as Trichosporon sp. DMI-5-1 based on microscopic morphology and 18S rDNA sequence. Comparative investigations on biodegradation of three isomers of DMPEs, namely dimethyl phthalate (DMP), dimethyl isophthalate (DMI), and dimethyl terephthalate (DMT), were carried out with this yeast strain. Trichosporon sp. DMI-5-1 could not mineralize DMPEs completely but transform them to respective monomethyl phthalate or phthalic acid. Biochemical degradation pathways for the three DMPE isomers by Trichosporon sp. DMI-5-1 were apparently different. The yeast carried out one-step ester hydrolysis of DMP and DMI to respective monoesters (monomethyl phthalate and monomethyl isophthalate, respectively) and no further metabolism of these two monoesters. Meanwhile, DMT was transformed by the yeast to monomethyl terephthalate and subsequently to terephthalic acid by stepwise hydrolysis of the two ester bonds. Conclusions: This study shows that different catalytic processes are involved in the transformation of DMPEs by the basidiomycetous yeast Trichosporon sp. DMI-5-1 and suggests that its esterases, responsible for the initial hydrolyzing the two ester bonds of DMPEs, are highly substrate specific. © 2011 Springer-Verlag.en_US
dc.languageengen_US
dc.publisherSpringer. The Journal's web site is located at http://www.springer.com/environment/journal/11356en_US
dc.relation.ispartofEnvironmental Science and Pollution Researchen_US
dc.rightsThe original publication is available at www.springerlink.com-
dc.subject.meshBiodegradation, Environmentalen_US
dc.subject.meshEnvironmental Monitoringen_US
dc.subject.meshEsters - Metabolismen_US
dc.subject.meshFungal Proteins - Isolation & Purificationen_US
dc.subject.meshGeologic Sediments - Chemistry - Microbiologyen_US
dc.subject.meshHydrolysisen_US
dc.subject.meshMycological Typing Techniquesen_US
dc.subject.meshPhthalic Acids - Chemistryen_US
dc.subject.meshPlasticizers - Chemistryen_US
dc.subject.meshRna, Ribosomal, 18S - Geneticsen_US
dc.subject.meshTrichosporon - Classification - Metabolismen_US
dc.titleComparison of initial hydrolysis of the three dimethyl phthalate esters (DMPEs) by a basidiomycetous yeast, Trichosporon DMI-5-1, from coastal sedimenten_US
dc.typeArticleen_US
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_US
dc.identifier.authorityGu, JD=rp00701en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s11356-011-0525-1en_US
dc.identifier.pmid21626443-
dc.identifier.scopuseid_2-s2.0-80053983325en_US
dc.identifier.hkuros209611-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80053983325&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume18en_US
dc.identifier.issue9en_US
dc.identifier.spage1653en_US
dc.identifier.epage1660en_US
dc.identifier.isiWOS:000298986200021-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridLuo, ZH=35206114900en_US
dc.identifier.scopusauthoridWu, YR=36195002000en_US
dc.identifier.scopusauthoridPang, KL=7101856049en_US
dc.identifier.scopusauthoridGu, JD=7403129601en_US
dc.identifier.scopusauthoridVrijmoed, LLP=7003337180en_US
dc.identifier.citeulike9444504-

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