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Article: Overexpression of arabidopsis acbp3 enhances npr1-dependent plant resistance to pseudomonas syringe pv tomato dc3000

TitleOverexpression of arabidopsis acbp3 enhances npr1-dependent plant resistance to pseudomonas syringe pv tomato dc3000
Authors
Issue Date2011
PublisherAmerican Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.org
Citation
Plant Physiology, 2011, v. 156 n. 4, p. 2069-2081 How to Cite?
AbstractACBP3 is one of six Arabidopsis (Arabidopsis thaliana) genes, designated ACBP1 to ACBP6, that encode acyl-coenzyme A (CoA)-binding proteins (ACBPs). These ACBPs bind long-chain acyl-CoA esters and phospholipids and are involved in diverse cellular functions, including acyl-CoA homeostasis, development, and stress tolerance. Recombinant ACBP3 binds polyunsaturated acyl-CoA esters and phospholipids in vitro. Here, we show that ACBP3 plays a role in the plant defense response to the bacterial pathogen Pseudomonas syringae pv tomato DC3000. ACBP3 mRNA was up-regulated upon pathogen infection and treatments using pathogen elicitors and defense-related phytohormones. Transgenic Arabidopsis ACBP3 overexpressors (ACBP3-OEs) showed constitutive expression of pathogenesis-related genes (PR1, PR2, and PR5), cell death, and hydrogen peroxide accumulation in leaves. Consequently, ACBP3-OEs displayed enhanced resistance to the bacterial pathogen P. syringae DC3000. In contrast, the acbp3 T-DNA insertional mutant was more susceptible and exhibited lower PR gene transcript levels upon infection. Using the ACBP3 OE-1 line in combination with nonexpressor of PR genes1 (npr1-5) or coronatine-insensitive1 (coi1-2), we concluded that the enhanced PR gene expression and P. syringae DC3000 resistance in the ACBP3-OEs are dependent on the NPR1-mediated, but not the COI1-mediated, signaling pathway. Given that ACBP3-OEs showed greater susceptibility to infection by the necrotrophic fungus Botrytis cinerea while the acbp3 mutant was less susceptible, we suggest that ACBP3 plays a role in the plant defense response against biotrophic pathogens that is distinct from necrotrophic pathogens. ACBP3 function in plant defense was supported further by bioinformatics data showing up-regulation of many biotic and abiotic stress-related genes in ACBP3 OE-1 in comparison with the wild type. © 2011 American Society of Plant Biologists.
Persistent Identifierhttp://hdl.handle.net/10722/179248
ISSN
2015 Impact Factor: 6.28
2015 SCImago Journal Rankings: 3.642
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXiao, Sen_US
dc.contributor.authorChye, MLen_US
dc.date.accessioned2012-12-19T09:53:22Z-
dc.date.available2012-12-19T09:53:22Z-
dc.date.issued2011en_US
dc.identifier.citationPlant Physiology, 2011, v. 156 n. 4, p. 2069-2081en_US
dc.identifier.issn0032-0889en_US
dc.identifier.urihttp://hdl.handle.net/10722/179248-
dc.description.abstractACBP3 is one of six Arabidopsis (Arabidopsis thaliana) genes, designated ACBP1 to ACBP6, that encode acyl-coenzyme A (CoA)-binding proteins (ACBPs). These ACBPs bind long-chain acyl-CoA esters and phospholipids and are involved in diverse cellular functions, including acyl-CoA homeostasis, development, and stress tolerance. Recombinant ACBP3 binds polyunsaturated acyl-CoA esters and phospholipids in vitro. Here, we show that ACBP3 plays a role in the plant defense response to the bacterial pathogen Pseudomonas syringae pv tomato DC3000. ACBP3 mRNA was up-regulated upon pathogen infection and treatments using pathogen elicitors and defense-related phytohormones. Transgenic Arabidopsis ACBP3 overexpressors (ACBP3-OEs) showed constitutive expression of pathogenesis-related genes (PR1, PR2, and PR5), cell death, and hydrogen peroxide accumulation in leaves. Consequently, ACBP3-OEs displayed enhanced resistance to the bacterial pathogen P. syringae DC3000. In contrast, the acbp3 T-DNA insertional mutant was more susceptible and exhibited lower PR gene transcript levels upon infection. Using the ACBP3 OE-1 line in combination with nonexpressor of PR genes1 (npr1-5) or coronatine-insensitive1 (coi1-2), we concluded that the enhanced PR gene expression and P. syringae DC3000 resistance in the ACBP3-OEs are dependent on the NPR1-mediated, but not the COI1-mediated, signaling pathway. Given that ACBP3-OEs showed greater susceptibility to infection by the necrotrophic fungus Botrytis cinerea while the acbp3 mutant was less susceptible, we suggest that ACBP3 plays a role in the plant defense response against biotrophic pathogens that is distinct from necrotrophic pathogens. ACBP3 function in plant defense was supported further by bioinformatics data showing up-regulation of many biotic and abiotic stress-related genes in ACBP3 OE-1 in comparison with the wild type. © 2011 American Society of Plant Biologists.en_US
dc.languageengen_US
dc.publisherAmerican Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.orgen_US
dc.relation.ispartofPlant Physiologyen_US
dc.subject.meshArabidopsis - Drug Effects - Genetics - Immunology - Microbiologyen_US
dc.subject.meshArabidopsis Proteins - Genetics - Metabolismen_US
dc.subject.meshArachidonic Acid - Pharmacologyen_US
dc.subject.meshBotrytis - Drug Effects - Physiologyen_US
dc.subject.meshCarrier Proteins - Genetics - Metabolismen_US
dc.subject.meshCell Death - Drug Effectsen_US
dc.subject.meshDisease Resistance - Drug Effects - Immunologyen_US
dc.subject.meshGene Expression Regulation, Plant - Drug Effectsen_US
dc.subject.meshGene Knockout Techniquesen_US
dc.subject.meshGenes, Plant - Geneticsen_US
dc.subject.meshGreen Fluorescent Proteins - Metabolismen_US
dc.subject.meshHydrogen Peroxide - Metabolismen_US
dc.subject.meshIntracellular Space - Drug Effects - Metabolismen_US
dc.subject.meshOligonucleotide Array Sequence Analysisen_US
dc.subject.meshPhenotypeen_US
dc.subject.meshPlant Diseases - Immunology - Microbiologyen_US
dc.subject.meshPlant Growth Regulators - Pharmacologyen_US
dc.subject.meshPseudomonas Syringae - Drug Effects - Physiologyen_US
dc.subject.meshRna, Messenger - Genetics - Metabolismen_US
dc.subject.meshSalicylic Acid - Metabolismen_US
dc.titleOverexpression of arabidopsis acbp3 enhances npr1-dependent plant resistance to pseudomonas syringe pv tomato dc3000en_US
dc.typeArticleen_US
dc.identifier.emailXiao, S: xiaoshi@graduate.hku.hken_US
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_US
dc.identifier.authorityXiao, S=rp00817en_US
dc.identifier.authorityChye, ML=rp00687en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1104/pp.111.176933en_US
dc.identifier.pmid21670223-
dc.identifier.pmcidPMC3149925-
dc.identifier.scopuseid_2-s2.0-79961174645en_US
dc.identifier.hkuros198562-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79961174645&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume156en_US
dc.identifier.issue4en_US
dc.identifier.spage2069en_US
dc.identifier.epage2081en_US
dc.identifier.eissn1532-2548-
dc.identifier.isiWOS:000293568800032-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridXiao, S=7402022635en_US
dc.identifier.scopusauthoridChye, ML=7003905460en_US

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