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Article: Survival, Acid and Bile Tolerance, and Surface Hydrophobicity of Microencapsulated B. animalis ssp. lactis Bb12 during Storage at Room Temperature

TitleSurvival, Acid and Bile Tolerance, and Surface Hydrophobicity of Microencapsulated B. animalis ssp. lactis Bb12 during Storage at Room Temperature
Authors
Issue Date2011
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-1147
Citation
Journal of Food Science, 2011, v. 76 n. 9, p. M592-M599 How to Cite?
AbstractSurvival, acid and bile tolerance, and surface hydrophobicity of microencapsulated Bifidobacterium animalis ssp. lactis Bb12 were studied during storage at room temperature (25 °C) at low water activity (0.07, 0.1, and 0.2). Two types of alginate-based systems were prepared with and without mannitol as microencapsulant of B. animalis ssp. lactis Bb12. Formation of gel beads containing cells was achieved by dropping each emulsion into CaCl 2 solution; then, the beads were freeze dried. Survival, acid tolerance during 2-h exposure in de Man, Rogosa, Sharpe (MRS) broth at pH 2.0, bile tolerance during 8-h exposure in MRS broth containing taurocholic acid at pH 5.8, and retention of surface hydrophobicity were determined after freeze drying and during storage. The result showed that neither alginate nor alginate-mannitol formulation was effective in protecting B. animalis ssp. lactis Bb12 during freezing and freeze drying. The viability in alginate-mannitol and alginate formulations after freeze drying was 6.61 and 6.34 log CFU/g, respectively. Storage at low a w improved survival, acid tolerance, bile tolerance, and surface hydrophobicity retention of microencapsulated B. animalis ssp. lactis Bb12 when compared with controlled storage in an aluminum foil (with a w of 0.38 and 0.40 for alginate-mannitol and alginate formulations, respectively). Alginate mannitol was more effective than the alginate system during a short period of storage, but its effectiveness decreased during a long period of storage (80% survival at 10 wk). Nevertheless, storage of microencapsulated B. animalis ssp. lactis Bb12 in an aluminum foil without a w adjustment during 10 wk at room temperature was not effective (survival was 64% to 65%). © 2011 Institute of Food Technologists ®.
Persistent Identifierhttp://hdl.handle.net/10722/179247
ISSN
2015 Impact Factor: 1.649
2015 SCImago Journal Rankings: 0.839
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDianawati, Den_US
dc.contributor.authorShah, NPen_US
dc.date.accessioned2012-12-19T09:53:22Z-
dc.date.available2012-12-19T09:53:22Z-
dc.date.issued2011en_US
dc.identifier.citationJournal of Food Science, 2011, v. 76 n. 9, p. M592-M599en_US
dc.identifier.issn0022-1147en_US
dc.identifier.urihttp://hdl.handle.net/10722/179247-
dc.description.abstractSurvival, acid and bile tolerance, and surface hydrophobicity of microencapsulated Bifidobacterium animalis ssp. lactis Bb12 were studied during storage at room temperature (25 °C) at low water activity (0.07, 0.1, and 0.2). Two types of alginate-based systems were prepared with and without mannitol as microencapsulant of B. animalis ssp. lactis Bb12. Formation of gel beads containing cells was achieved by dropping each emulsion into CaCl 2 solution; then, the beads were freeze dried. Survival, acid tolerance during 2-h exposure in de Man, Rogosa, Sharpe (MRS) broth at pH 2.0, bile tolerance during 8-h exposure in MRS broth containing taurocholic acid at pH 5.8, and retention of surface hydrophobicity were determined after freeze drying and during storage. The result showed that neither alginate nor alginate-mannitol formulation was effective in protecting B. animalis ssp. lactis Bb12 during freezing and freeze drying. The viability in alginate-mannitol and alginate formulations after freeze drying was 6.61 and 6.34 log CFU/g, respectively. Storage at low a w improved survival, acid tolerance, bile tolerance, and surface hydrophobicity retention of microencapsulated B. animalis ssp. lactis Bb12 when compared with controlled storage in an aluminum foil (with a w of 0.38 and 0.40 for alginate-mannitol and alginate formulations, respectively). Alginate mannitol was more effective than the alginate system during a short period of storage, but its effectiveness decreased during a long period of storage (80% survival at 10 wk). Nevertheless, storage of microencapsulated B. animalis ssp. lactis Bb12 in an aluminum foil without a w adjustment during 10 wk at room temperature was not effective (survival was 64% to 65%). © 2011 Institute of Food Technologists ®.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-1147en_US
dc.relation.ispartofJournal of Food Scienceen_US
dc.subject.meshAlginates - Metabolismen_US
dc.subject.meshBifidobacterium - Growth & Development - Isolation & Purificationen_US
dc.subject.meshBile - Microbiologyen_US
dc.subject.meshColony Count, Microbialen_US
dc.subject.meshDrug Compoundingen_US
dc.subject.meshFood Microbiologyen_US
dc.subject.meshFood Preservation - Methodsen_US
dc.subject.meshFood Storage - Methodsen_US
dc.subject.meshFreeze Dryingen_US
dc.subject.meshGastric Acid - Metabolismen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshHydrophobic And Hydrophilic Interactionsen_US
dc.subject.meshMannitol - Metabolismen_US
dc.subject.meshMicrobial Viabilityen_US
dc.subject.meshProbioticsen_US
dc.subject.meshTemperatureen_US
dc.titleSurvival, Acid and Bile Tolerance, and Surface Hydrophobicity of Microencapsulated B. animalis ssp. lactis Bb12 during Storage at Room Temperatureen_US
dc.typeArticleen_US
dc.identifier.emailShah, NP: npshah@hku.hken_US
dc.identifier.authorityShah, NP=rp01571en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1750-3841.2011.02422.xen_US
dc.identifier.pmid22416710-
dc.identifier.scopuseid_2-s2.0-79961128260en_US
dc.identifier.hkuros205695-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79961128260&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume76en_US
dc.identifier.issue9en_US
dc.identifier.spageM592en_US
dc.identifier.epageM599en_US
dc.identifier.isiWOS:000297457000027-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridDianawati, D=40761270000en_US
dc.identifier.scopusauthoridShah, NP=7401823907en_US

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