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Article: Structures of clusters in sweetpotato amylopectin

TitleStructures of clusters in sweetpotato amylopectin
Authors
Issue Date2011
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/carres
Citation
Carbohydrate Research, 2011, v. 346 n. 9, p. 1112-1121 How to Cite?
AbstractSweetpotato amylopectin was subjected to partial hydrolysis by α-amylase from Bacillus amyloliquefaciens to release the clusters. Clusters were then fractionated and precipitated by methanol and structurally characterized by gel-permeation chromatography and high-performance anion-exchange chromatography. An initial stage of α-amylolysis on the amylopectin isolated mostly domains but also clusters. A second stage of α-amylolysis on the domains and clusters further isolated their respective clusters and sub-clusters. All the domains, clusters and sub-clusters were sequentially subjected to phosphorolysis and β-amylolysis to obtain their internal part. The degree of polymerization of the clusters in the form of φ,β-limit dextrins were from 58 to 86. Each domain contained 2-8 clusters. Two types of clusters were structurally identified. Type A clusters were larger and contained about 12 chains per cluster with higher degree of branching (DB), whereas those of type B were smaller and contained about eight chains per cluster with lower DB. © 2011 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/179233
ISSN
2015 Impact Factor: 1.817
2015 SCImago Journal Rankings: 0.612
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhu, Fen_US
dc.contributor.authorCorke, Hen_US
dc.contributor.authorÅman, Pen_US
dc.contributor.authorBertoft, Een_US
dc.date.accessioned2012-12-19T09:53:17Z-
dc.date.available2012-12-19T09:53:17Z-
dc.date.issued2011en_US
dc.identifier.citationCarbohydrate Research, 2011, v. 346 n. 9, p. 1112-1121en_US
dc.identifier.issn0008-6215en_US
dc.identifier.urihttp://hdl.handle.net/10722/179233-
dc.description.abstractSweetpotato amylopectin was subjected to partial hydrolysis by α-amylase from Bacillus amyloliquefaciens to release the clusters. Clusters were then fractionated and precipitated by methanol and structurally characterized by gel-permeation chromatography and high-performance anion-exchange chromatography. An initial stage of α-amylolysis on the amylopectin isolated mostly domains but also clusters. A second stage of α-amylolysis on the domains and clusters further isolated their respective clusters and sub-clusters. All the domains, clusters and sub-clusters were sequentially subjected to phosphorolysis and β-amylolysis to obtain their internal part. The degree of polymerization of the clusters in the form of φ,β-limit dextrins were from 58 to 86. Each domain contained 2-8 clusters. Two types of clusters were structurally identified. Type A clusters were larger and contained about 12 chains per cluster with higher degree of branching (DB), whereas those of type B were smaller and contained about eight chains per cluster with lower DB. © 2011 Elsevier Ltd. All rights reserved.en_US
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/carresen_US
dc.relation.ispartofCarbohydrate Researchen_US
dc.subject.meshAmylopectin - Chemistryen_US
dc.subject.meshCarbohydrate Conformationen_US
dc.subject.meshChemical Fractionationen_US
dc.subject.meshIpomoea Batatas - Chemistryen_US
dc.titleStructures of clusters in sweetpotato amylopectinen_US
dc.typeArticleen_US
dc.identifier.emailCorke, H: harold@hku.hken_US
dc.identifier.authorityCorke, H=rp00688en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.carres.2011.03.001en_US
dc.identifier.pmid21507388-
dc.identifier.scopuseid_2-s2.0-79955932541en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79955932541&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume346en_US
dc.identifier.issue9en_US
dc.identifier.spage1112en_US
dc.identifier.epage1121en_US
dc.identifier.isiWOS:000291417000005-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridZhu, F=35306203800en_US
dc.identifier.scopusauthoridCorke, H=7007102942en_US
dc.identifier.scopusauthoridÅman, P=7004451997en_US
dc.identifier.scopusauthoridBertoft, E=6604042426en_US
dc.identifier.citeulike8987785-

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