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Article: Optimization of nitrogen source for enhanced production of squalene from thraustochytrid Aurantiochytrium sp

TitleOptimization of nitrogen source for enhanced production of squalene from thraustochytrid Aurantiochytrium sp
Authors
Issue Date2010
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/713354/description#description
Citation
New Biotechnology, 2010, v. 27 n. 4, p. 382-389 How to Cite?
AbstractNitrogen (N) sources, the critical medium component, were optimized for squalene production by microalga Aurantiochytrium sp. in heterotrophic cultures. In screening experiments monosodium glutamate, yeast extract and tryptone were found to enhance cell growth and squalene production. The optimal levels of the three nitrogen sources were further determined through central composite experimental design. The squalene content and yield were both influenced not only by monosodium glutamate, tryptone and yeast extract, but also by their interactions. The squalene content and squalene yield were described by the second-order polynomial equations with high confidence levels (>99%). The optimal concentrations of monosodium glutamate, yeast extract and tryptone were predicted to be 6.61. g/L, 6.13. g/L and 4.50. g/L for squalene content and 6.94. g/L, 6.22. g/L and 4.40. g/L for squalene yield, respectively. In the verification experiment, the squalene content and squalene yield reached 0.72. mg/g and 5.90. mg/L, respectively, which were much higher than those obtained in previous studies. © 2010 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/179205
ISSN
2015 Impact Factor: 3.199
2015 SCImago Journal Rankings: 1.092
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Gen_US
dc.contributor.authorFan, KWen_US
dc.contributor.authorLu, FPen_US
dc.contributor.authorLi, Qen_US
dc.contributor.authorAki, Ten_US
dc.contributor.authorChen, Fen_US
dc.contributor.authorJiang, Yen_US
dc.date.accessioned2012-12-19T09:52:55Z-
dc.date.available2012-12-19T09:52:55Z-
dc.date.issued2010en_US
dc.identifier.citationNew Biotechnology, 2010, v. 27 n. 4, p. 382-389en_US
dc.identifier.issn1871-6784en_US
dc.identifier.urihttp://hdl.handle.net/10722/179205-
dc.description.abstractNitrogen (N) sources, the critical medium component, were optimized for squalene production by microalga Aurantiochytrium sp. in heterotrophic cultures. In screening experiments monosodium glutamate, yeast extract and tryptone were found to enhance cell growth and squalene production. The optimal levels of the three nitrogen sources were further determined through central composite experimental design. The squalene content and yield were both influenced not only by monosodium glutamate, tryptone and yeast extract, but also by their interactions. The squalene content and squalene yield were described by the second-order polynomial equations with high confidence levels (>99%). The optimal concentrations of monosodium glutamate, yeast extract and tryptone were predicted to be 6.61. g/L, 6.13. g/L and 4.50. g/L for squalene content and 6.94. g/L, 6.22. g/L and 4.40. g/L for squalene yield, respectively. In the verification experiment, the squalene content and squalene yield reached 0.72. mg/g and 5.90. mg/L, respectively, which were much higher than those obtained in previous studies. © 2010 Elsevier B.V.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/713354/description#descriptionen_US
dc.relation.ispartofNew Biotechnologyen_US
dc.subject.meshBiomassen_US
dc.subject.meshCulture Media - Pharmacologyen_US
dc.subject.meshModels, Biologicalen_US
dc.subject.meshNitrogen - Pharmacologyen_US
dc.subject.meshRegression Analysisen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.subject.meshSqualene - Metabolismen_US
dc.subject.meshSurface Properties - Drug Effectsen_US
dc.titleOptimization of nitrogen source for enhanced production of squalene from thraustochytrid Aurantiochytrium spen_US
dc.typeArticleen_US
dc.identifier.emailChen, F: sfchen@hku.hken_US
dc.identifier.authorityChen, F=rp00672en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.nbt.2010.04.005en_US
dc.identifier.pmid20412873-
dc.identifier.scopuseid_2-s2.0-77955095940en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77955095940&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume27en_US
dc.identifier.issue4en_US
dc.identifier.spage382en_US
dc.identifier.epage389en_US
dc.identifier.isiWOS:000281177000016-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridChen, G=13406780100en_US
dc.identifier.scopusauthoridFan, KW=7202978325en_US
dc.identifier.scopusauthoridLu, FP=8937315400en_US
dc.identifier.scopusauthoridLi, Q=27171864800en_US
dc.identifier.scopusauthoridAki, T=7006453167en_US
dc.identifier.scopusauthoridChen, F=7404907980en_US
dc.identifier.scopusauthoridJiang, Y=24605346600en_US

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